MicroRNA derived from myeloid-derived suppressor cell might contribute to the pathogenesis concerning immunosuppression in sepsis

• Project/Area Number: 18K16525
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Kansai Medical University
• Principal Investigator: NISHIMOTO Kota 関西医科大学, 医学部, 助教 (30758850)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: MicroRNA / 骨髄由来抑制細胞 / sepsis / immunosuppression / 遺伝子治療 / 敗血症

Outline of Final Research Achievements

In the first year, we evaluated the time course changes of comprehensive microRNA expression by the next-generation sequencing after extraction of blood and peritoneal macrophage of rat sepsis model using lipopolysaccharide injection. Subsequent next year, we have extracted rat myeloid-derived suppressor cell from blood and peritoneal cavity by positive selection of the cell surface marker. Thereafter, we evaluated the time course changes of comprehensive microRNA expression by the next-generation sequencing. Changes in microRNA expression were observed in rat macrophages and myeloid-derived suppressor cells in rats under sepsis compared with rats by saline infusion(control group)
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Academic Significance and Societal Importance of the Research Achievements

１つのmiRNAが複数の遺伝子の発現に関与していることから、今まで１つのタンパク質の発現変化で説明が出来なかったCommon Diseaseの病態解明、バイオマーカー、治療薬としてmiRNAの解析は期待できる。報告者らは、敗血症病態時の骨髄由来抑制細胞が産生するmiRNAが重要な役割を担うと考える。

Research Products

• [Journal Article] Effectiveness of combined antithrombin and thrombomodulin therapy on in-hospital mortality in mechanically ventilated septic patients with disseminated intravascular coagulation (2020)
  • Author(s): Umegaki Takeshi、Kunisawa Susumu、Nishimoto Kota、Kamibayashi Takahiko、Imanaka Yuichi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 4874-4874
  • DOI: 10.1038/s41598-020-61809-2
  • Peer Reviewed / Open Access
• [Presentation] 敗血症症例におけるガンマグロブリン製剤投与後IgG値に関する検討 (2019)
  • Author(s): 梅垣岳志, 中島友理奈, 添田岳宏, 西本浩太, 安藤亜希子, 穴田夏樹, 奥 佳菜子, 右馬猛生, 正司智洋, 楠 宗矩, 中嶋康文, 萩平 哲, 上林卓彦
  • Organizer: 日本集中治療医学会第3回関西支部学術集会
• [Presentation] 敗血症症例の初期IgG値と退院時転帰の関連についての検討 (2019)
  • Author(s): 角 千里, 梅垣岳志, 中島友理奈, 添田岳宏, 西本浩太, 博多紗綾, 右馬猛生, 中嶋康文, 萩平 哲, 上林卓彦
  • Organizer: 第46回日本集中治療医学会学術集会