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Functional evaluation of transplanted neural stem cells by optgenetics

Research Project

Project/Area Number 18K16594
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56010:Neurosurgery-related
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Umebayashi Daisuke  京都府立医科大学, 医学(系)研究科(研究院), 助教 (90635575)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsチャネルロドプシン / ハロロドプシン / プラスミド / ウイルスベクター / 神経幹細胞 / トランスフェクション / 脊髄損傷 / 脳損傷 / オプトジェネティクス / 脊髄損傷モデル作成 / 環境整備 / 神経機能
Outline of Final Research Achievements

We established the mouse spinal cord injury model, following to regulate electrophysiological and motor score systems.
Neural stem cells were cultured and estimated their multipotency. Lentivirus of CS2-CAG-ChR2-mVenus-ST, CS2-CAG-eNpHR3.0-mRuby2-ST, and CS2-CAG-eNpHR3.0-FLAG-mRuby2-ST using HEK293FT cells were produced. These optgenetics are ready to transfect the neural stem cells. We are starting to evaluate these optogenetics in vivo.

Academic Significance and Societal Importance of the Research Achievements

光感受性遺伝子導入神経幹細胞を作成し、神経幹細胞の機能をリアルタイムに興奮・抑制させることにより、その神経再生への機序を明らかにする実験系への確立に貢献した。
これにより、神経幹細胞の再生効果が細胞自体の増殖によるものか液性因子によるものかの探索が今後期待でき得る。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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