| Project/Area Number |
18K16628
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56020:Orthopedics-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Scleraxis / Sox9 / enthesis healing / rotator cuff / scleraxis / enthesis / healing |
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| Outline of Final Research Achievements |
Scx+/Sox9+ cells, a specific multipotent cell population, have been shown to contribute to the enthesis formation during mouse embryonic development. We investigated the involvement of Scx+/Sox9+ cells in fibrocartilaginous (FC) enthesis healing using a supraspinatus enthesis healing model established in juvenile (3-week-old) ScxGFP Tg mice. In juvenile mice, Scx+/Sox9+ cells were widely distributed in injury site in early phase, and they were located near the surface of the repaired FC at 4 week post-injury. The spatiotemporal localization pattern of Scx+/Sox9+ cells in juvenile mice was similar to that in postnatal FC enthesis formation. These findings indicate that Scx+/Sox9+ cells may be able to act as entheseal progenitors in postnatal healing. Moreover, their healing action may be related to the TGF-beta/pSmad3 pathway, as evidenced by the higher pSmad3-positive cell count during healing.
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| Academic Significance and Societal Importance of the Research Achievements |
Scx+/Sox9+前駆細胞は発生期に腱・靱帯の付着部の形成に寄与することが示されている。本研究では, 幼若マウスの腱板付着部損傷後の線維軟骨層の修復にScx+/Sox9+細胞が寄与ししている可能性を示唆させる結果を得た。これは, 内在性のScx+/Sox9+細胞の修復部への動員が線維軟骨修復に関連する可能性性を示唆する結果であり, 成体におけるScx+/Sox9+細胞を介した新たな修復促進治療につながる知見となることが期待される。
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