| Project/Area Number |
18K16718
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56030:Urology-related
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| Research Institution | Hirosaki University |
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Principal Investigator |
Noro Daisuke 弘前大学, 医学研究科, 客員研究員 (60793635)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | オステオポンチン / 糖鎖 / レクチン / 尿路結石 |
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| Outline of Final Research Achievements |
Osteopontin (OPN) is a matrix glycoprotein of urinary calculi. This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis. We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 157 urolithiasis patients and 110 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment. This study showed that OPN concentration in urine was significantly lower in stone forming patients than in HVs. However, Gal3C-S-OPN was significantly increased in stone forming patients, which suggested its potential as a biomarker of urolithiasis formation. Therefore, Gal3C-S-OPN/uFL-OPN levels might be a biomarker of stone formation. Urolithiasis is a disease with a high rate of recurrence. Gal3C-S-OPN might be useful as a predictor of recurrence, and elucidation of the mechanism of urinary stone recurrence may lead to prevention.
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| Academic Significance and Societal Importance of the Research Achievements |
我が国では100人中6人、欧米では20人にも達する国もみられ、生産年齢の男性に多く、その成因の究明と再発予防法の確立は急務である。本研究で得られた成果として、尿路結石患者では健常者と比較して、尿中OPN濃度は有意に減少したにも関わらず糖鎖変異OPN(Gal3C-S-OPN)が有意に増加していた。尿路結石の有無とGal3C-S-OPN量が関連していることから、OPN糖鎖変異が尿路結石診断、再発予測に関する糖鎖性バイオマーカーに成り得る可能性が示唆された。尿路結石は、再発が多く結石形成の再発予測ができれば、患者のQOLを改善するバイオマーカーとなり、社会的意義は、非常に大きい。
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