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Regulatory mechanism of cell differentiation in the endolymphatic sac

Research Project

Project/Area Number 18K16876
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56050:Otorhinolaryngology-related
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Keiji Honda  東京医科歯科大学, 医学部附属病院, 助教 (90621079)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords内リンパ嚢 / イオノサイト / 細胞分化 / 前庭水管拡大 / 先天性難聴 / 組織培養 / Notchシグナル伝達系
Outline of Final Research Achievements

The endolymphatic sac is essential for the normal development and function of the inner ear, and its abnormality causes inner ear diseases such as enlarged vestibular aqueduct. To elucidate the cell differentiation mechanism of mitochondria-rich cells and ribosome-rich cells, which are the two cell types constituting the endolymphatic sac, we attempted to observe the effects of knockdown of candidate genes for transcriptional regulation on cell differentiation in mouse endolymphatic sac tissue culture. Although we tried various protocols, contraction of epithelial tissues occurred during culture of the endolymphatic sac, leading to problems in maintaining a stable morphology that could be examined under a microscope. It is thought that ion transport and water absorption by MRC may have affected the morphology of the cells.

Academic Significance and Societal Importance of the Research Achievements

内リンパ嚢細胞の分化機構の解明は前庭水管拡大症の治療法の確立に極めて重要な意義を持つ。たとえば、イオン輸送関連遺伝子の変異によりタンパクレベルでイオン輸送機能低下を有している場合においても、前駆細胞からMRCへの分化を誘導させその細胞数を増加させることによって、機能低下を補完できる可能性がある。また別の可能性として、ES/iPS細胞からMRCに分化誘導する手法を構築し、治療へつなげることも考えられる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2021

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Patient-specific virtual and mixed reality for?immersive, experiential anatomy education and for surgical planning in?temporal bone surgery2021

    • Author(s)
      Yamazaki Ayame、Ito Taku、Sugimoto Maki、Yoshida Soichiro、Honda Keiji、Kawashima Yoshiyuki、Fujikawa Taro、Fujii Yasuhisa、Tsutsumi Takeshi
    • Journal Title

      Auris Nasus Larynx

      Volume: 48 Issue: 6 Pages: 1081-1091

    • DOI

      10.1016/j.anl.2021.03.009

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissection of the Endolymphatic Sac from Mice.2021

    • Author(s)
      Honda K, Lee HJ, Griffith AJ, Roux I
    • Journal Title

      Journal of visualized experiments : JoVE

      Volume: 169 Issue: 169

    • DOI

      10.3791/62375

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A case of omalizumab-associated eosinophilic granulomatosis with polyangiitis.2021

    • Author(s)
      Yamaki Y, Hayashi D, Honda K, Imai H, Takada H
    • Journal Title

      Pediatrics international : official journal of the Japan Pediatric Society

      Volume: 63 Issue: 9 Pages: 1119-1121

    • DOI

      10.1111/ped.14540

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic architecture and phenotypic landscape of SLC26A4-related hearing loss2021

    • Author(s)
      Honda Keiji, Griffith Andrew J.
    • Journal Title

      HUMAN GENETICS

      Volume: 141 Issue: 3-4 Pages: 455-464

    • DOI

      10.1007/s00439-021-02311-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cochlear Pathomorphogenesis of Incomplete Partition Type II in Slc26a4-Null Mice2021

    • Author(s)
      Ito Taku、Fujikawa Taro、Honda Keiji、Makabe Ayane、Watanabe Hiroki、Bai Jing、Kawashima Yoshiyuki、Miwa Toru、Griffith Andrew J.、Tsutsumi Takeshi
    • Journal Title

      Journal of the Association for Research in Otolaryngology

      Volume: 22 Issue: 6 Pages: 681

    • DOI

      10.1007/s10162-021-00812-4

    • URL

      https://pure.teikyo.jp/en/publications/efb6983c-e726-4c31-8488-ac44ee75d44d

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 【必読!メニエール病の新分類とその周辺疾患】メニエール病の周辺疾患の診断・治療 前庭性発作症2021

    • Author(s)
      本田 圭司
    • Journal Title

      耳鼻咽喉科・頭頸部外科

      Volume: 93 Pages: 1004-1007

    • Related Report
      2021 Annual Research Report
  • [Presentation] 1年の経過で著明な内リンパ水腫縮小を認めたメニエール病確定診断例の1例2021

    • Author(s)
      古賀 千佳子, 本田 圭司, 渡邊 浩基, 大岡 知樹, 山崎 あやめ, 鈴木 康弘, 堤 剛
    • Organizer
      Equilibrium Research
    • Related Report
      2021 Annual Research Report
  • [Presentation] メニエール病確定診断例の臨床像と内リンパ水腫の進展様式2021

    • Author(s)
      本田 圭司, 渡邊 浩基, 大岡 知樹, 山崎 あやめ, 鈴木 康弘, 堤 剛
    • Organizer
      Equilibrium Research
    • Related Report
      2021 Annual Research Report
  • [Presentation] 左椎骨動脈閉塞に合併した左メニエール病確定診断例の1例2021

    • Author(s)
      梶野 晃雅, 本田 圭司, 渡邊 浩基, 大岡 知樹, 山崎 あやめ, 鈴木 康弘, 堤 剛
    • Organizer
      Equilibrium Research
    • Related Report
      2021 Annual Research Report
  • [Book] Enlarged Vestibular Aqueduct In: 5 (Second Edition), edited by M. M. Lesperance2021

    • Author(s)
      Griffith AJ and Honda K
    • Total Pages
      577
    • Publisher
      Elsevier
    • ISBN
      9780323696180
    • Related Report
      2021 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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