Expression profiles of lncRNA in keloid fibroblasts
| Project/Area Number |
18K17005
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56070:Plastic and reconstructive surgery-related
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| Research Institution | Nagoya University (2020)
Nippon Medical School (2018-2019) |
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Principal Investigator |
Aoki Masayo 名古屋大学, 医学系研究科, 特任講師 (40465282)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | lncRNA / ケロイド / 細胞外マトリックス / 線維芽細胞 / マトリックス分解酵素 / 創傷治癒 |
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| Outline of Final Research Achievements |
Long non-coding RNA was comprehensively analyzed in keloid fibroblasts, which are intractable cutaneous fibrotic lesions. The gene expression of matrix metalloproteinase (MMP)-1,3, which is an enzyme that degrades extracellular matrix, is decreased in keloids. lncRNA encoded near chromosome 11q22.2, where these enzymes are located, was analyzed. The increased expression of LINC00900 (5.9-fold) was confirmed. It was suggested that LINC00900 has some function in fibrotic diseases.
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| Academic Significance and Societal Importance of the Research Achievements |
lncRNAは、タンパクをコードしないものの、生体の様々なプロセスに関与する。しかし、大部分のlncRNAの機能は未だ不明である。今回の研究課題で、これまで少なかったケロイド線維芽細胞（継代0代）における遺伝子発現プロファイルを構築することができた。これにより、ケロイドに関与する機能性lncRNA研究の発展が期待される。さらに、他の臓器の線維性疾患における機能性lncRNA研究への応用や、未だ機能が不明であるlncRNAの機能特定に貢献すると考えられる。
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Report
(4 results)
Research Products
(14 results)
