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Cell fate determination mechanism by acquisition of transcription factor Runx2 during fracture healing process

Research Project

Project/Area Number 18K17217
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57060:Surgical dentistry-related
Research InstitutionThe University of Tokyo

Principal Investigator

Miki Kashiwagi  東京大学, 医学部附属病院, 助教 (70803360)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
KeywordsRunx2 / 骨折治癒 / 骨疾患 / シングルセル解析 / 一細胞RNA-seq解析 / 骨修復 / 骨折 / ヘッジホッグ / Single Cell解析
Outline of Final Research Achievements

The purpose of this study was to elucidate the mechanism of the transcription factor Runt-related transcription factor 2 (Runx2), which is essential for embryonic bone development, and the function of hedgehog signaling in the fracture healing process. We created CAGCre-ERTM; Runx2fl / fl; R26RTomato mice and aimed to elucidate the factors involved in bone repair during the fracture healing. We also created a fracture model of Runx2 +/- mice and investigated the role of Runx2 in fracture healing. Furthermore, single cell analysis confirmed that the Runx2-positive cell population was continuously expressed from skeletal progenitor cells to mature osteoblasts.

Academic Significance and Societal Importance of the Research Achievements

超高齢社会を迎えた日本にとって、骨折などを含む骨疾患は社会的に取り組むべき大きな課題と考えられる。しかしながら、骨折治癒のメカニズムや治療法に関しては明らかとなっていない点も多数存在する。そこで、本研究による、骨折治癒のメカニズム(HhシグナルやRunx2の役割)が明らかとなれば、将来的な骨折を含む骨疾患の治療法に大きな進歩をもたらすと考えられる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results)

  • [Journal Article] Effects of Helioxanthin Derivative-Treated Human Dental Pulp Stem Cells on Fracture Healing2020

    • Author(s)
      D. Yamakawa, Y. Kawase-Koga, Y. Fujii, Y. Kanno, M. Sato, S. Ohba, Y. Kitaura, M. Kashiwagi, D. Chikazu
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 23 Pages: 9158-9171

    • DOI

      10.3390/ijms21239158

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficacy of Etanercept with Jaw-Training for Rheumatoid Arthritis with Firstly Temporomandibular Joint Symptoms: A Case Report2019

    • Author(s)
      Miki Kashiwagi , Takahiro Abe, Yuske Komiyama , Noriko Komatsu , Shoko Tateishi, Kazuto Hoshi
    • Journal Title

      Rheumatic Diseases and Treatment Journal

      Volume: 1 Pages: 6-9

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 成人Still病に併発した顎関節症状に対する治療経験2018

    • Author(s)
      柏木美樹、安部貴大、藤原夕子、小笠原徹、西條英人、星和人
    • Journal Title

      日本顎関節学会雑誌

      Volume: 30 Pages: 202-207

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 当科における13トリソミー・18トリソミーを有する口唇口蓋裂患者への取り組み2021

    • Author(s)
      柏木美樹
    • Organizer
      日本口蓋裂学会総会・学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 顔面動静脈血管奇形術後瘢痕に起因する開口障害に対し下顎骨区域切除術および人工下顎頭置換術を施行した1例2020

    • Author(s)
      柏木美樹,瀬田修平,藤原夕子,阿部雅修,安部貴大, 星和人
    • Organizer
      第210回(公社) 日本口腔外科学会関東支部学術集会
    • Related Report
      2020 Research-status Report
  • [Presentation] 両側口唇口蓋裂児における口腔内異物の1例2018

    • Author(s)
      柏木美樹、杉山円、菅家康介、西條英人、星和人
    • Organizer
      小児口腔外科学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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