Analysis of immune cells to delayed tooth movement in craniocerebral dysplasia

• Project/Area Number: 18K17240
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Tohoku University
• Principal Investigator: Bando Kanan 東北大学, 大学病院, 医員 (20772198)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 鎖骨頭蓋異形成症 / 免疫細胞

Outline of Final Research Achievements

In this study, we investigated the expression of Runx2 on immune cells in the oral cavity. Runx2 is known to be an essential transcription factor for osteoblasts, but its expression has also been confirmed in immune cells, although the expression of Runx2 in immune cells in the oral cavity has not been investigated. The expression of Runx2 in periodontal tissues was confirmed in dendritic cells and basophils. Next, we examined whether Runx2 expression was affected by mechanical stress. The expression of Runx2 in dendritic cells was increased by mechanical stress.

Academic Significance and Societal Importance of the Research Achievements

鎖骨頭蓋異形成症は骨牙細胞分化の必須転写因子であるRunx2の遺伝子変異による遺伝性疾患であり、顕著な歯の移動遅延が認められるため、矯正歯科治療が困難である。本研究は、新しい視点から鎖骨頭蓋異形成症の易感染性、歯の移動遅延の原因となる免疫細胞を同定、機能解析することにより、鎖骨頭蓋異形成症の顎顔面、口腔内病態を明確にし、新たな矯正歯科治療法の開発の基盤を提供することを目的とする。

Research Products

• [Journal Article] Basic Studies on the Mechanism, Prevention, and Treatment of Osteonecrosis of the Jaw Induced by Bisphosphonates (2020)
  • Author(s): Endo Yasuo、Funayama Hiromi、Yamaguchi Kouji、Monma Yuko、Yu Zhiqian、Deng Xue、Oizumi Takefumi、Shikama Yosuke、Tanaka Yukinori、Okada Satoshi、Kim Siyoung、Kiyama Tomomi、Bando Kanan、Shima Kazuhiro、Suzuki Hikari、Takahashi Tetsu
  • Journal Title: YAKUGAKU ZASSHI Volume: 140 Issue: 1 Pages: 63-79
  • DOI: 10.1248/yakushi.19-00125
  • NAID: 130007778763
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2020-01-01
  • Peer Reviewed / Open Access
• [Journal Article] Migratory dendritic cells in skin-draining lymph nodes have nickel-binding capabilities. (2020)
  • Author(s): 1.Toshinobu KUROISHI, Kanan BANDO, Reiska Kumala BAKTI, Gaku OUCHI, Yukinori TANAKA, Shunji SUGAWARA.
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 5050-5050
  • DOI: 10.1038/s41598-020-61875-6
  • Peer Reviewed / Open Access
• [Journal Article] Augmentation of Lipopolysaccharide-Induced Production of IL-1α and IL-1β in Mice Given Intravenous Zoledronate (a Nitrogen-Containing Bisphosphonate) and Its Prevention by Clodronate (a Non-nitrogen-containing Bisphosphonate) (2019)
  • Author(s): Suzuki H, Bando K, Tada H, Kiyama T, Oizumi T, Funayama H, Sugawara S, Takahashi T, Endo Y.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 42 Issue: 2 Pages: 164-172
  • DOI: 10.1248/bpb.b18-00408
  • NAID: 130007588866
  • ISSN: 0918-6158, 1347-5215
  • Year and Date: 2019-02-01
  • Peer Reviewed / Open Access
• [Journal Article] nterleukin-1 and histamine are essential for inducing nickel-allergy in mice. (2019)
  • Author(s): Bando Kanan, Kuroishi Toshinobu, Sugawara Shunji, Endo Yasuo.
  • Journal Title: Clinical & Experimental Allergy Volume: 49 Issue: 10 Pages: 1362-1373
  • DOI: 10.1111/cea.13467
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis induces the production of interleukin-31 by human mast cells, resulting in dysfunction of the gingival epithelial barrier. (2018)
  • Author(s): Tada Hiroyuki, Nishioka Takashi, Takase Aya, Numazaki Kento, Bando Kanan, Matsushita Kenji.
  • Journal Title: Cell Microbiology Volume: 21 Issue: 3 Pages: e12972-e12972
  • DOI: 10.1111/cmi.12972
  • Peer Reviewed