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Exploring common shifts in amino acid balance in periodontitis-associated microbial communities

Research Project

Project/Area Number 18K17281
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57080:Social dentistry-related
Research InstitutionOsaka University

Principal Investigator

Sakanaka Akito  大阪大学, 歯学研究科, 助教 (90815557)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords歯周病 / 微生物叢 / 栄養共生 / アミノ酸 / 歯垢微生物叢 / Dysbiosis / 代謝変動
Outline of Final Research Achievements

In this study, we aimed to elucidate the amino acid trophic webs in subgingibal microbial communities from both in vitro and clinical approaches. We showed that Fusobacterium nucleatum has the high production capability of polyamines, which is strengthend by coexistence with commensal microbes such as oral streptococci and Veillonella species.In clinical studies, we found several aromatic amino acid degradation products as metabolites that correlated with periodontal disease severity. We also found evidence suggesting that they were produced by Porphyromonas gingivalis and Prevotella spp. bacteria. These novel findings suggest that shifts in amino acid metabolism in subgingival communities underlie periodontal pathogenesis.

Academic Significance and Societal Importance of the Research Achievements

歯周病はバイオフィルムの量および質の変化に起因する慢性炎症性疾患である。近年、バイオフィルムの質の変化、特に、バイオフィルム内の菌種を超えた代謝活動が病原性を表す一つの尺度として注目されている。バイオフィルムにおける代謝活動の本体は、様々な微生物種間で行われる代謝物の受け渡し、すなわち栄養共生であり、これらのネットワークの総和として集団の代謝が形作られる。本研究の結果から、細菌集団内でのアミノ酸代謝変動が病態形成に関与する可能性を見出した。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Phosphoryl saccharides enhance ion availability and fluoroapatite formation.2019

    • Author(s)
      Tanaka, Kobayashi T, Tamenori Y, Sakanaka A, Kamasaka H, Kuriki T, Amano A
    • Journal Title

      Archives of Oral Biology

      Volume: 101 Pages: 135-141

    • DOI

      10.1016/j.archoralbio.2019.03.018

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細菌叢代謝ネットワークからみる歯周病の病因論(再掲載)2019

    • Author(s)
      坂中哲人
    • Journal Title

      Precision Medicine

      Volume: 2 Pages: 86-91

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 細菌叢代謝ネットワークからみる歯周病の病因論2018

    • Author(s)
      坂中哲人、久保庭雅恵、天野敦雄
    • Journal Title

      別冊BIO Clinica

      Volume: 7 Pages: 116-121

    • Related Report
      2018 Research-status Report
  • [Presentation] Role of pantothenate biosynthesis in starvation response and biofilm formation of Porphyromonas gingivalis2019

    • Author(s)
      Sakanaka A, Kuboniwa M, Ishikawa A, Mayumi S, Amano A
    • Organizer
      第68回日本口腔衛生学会・総会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Porphyromonas gingivalisのバイオフィルム形成におけるポリアミン類の影響の検討2019

    • Author(s)
      綿井大輔、坂中哲人、久保庭雅恵、福崎英一郎
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Pantothenate Metabolism Involves Long-term Survival and Virulence of Porphyromonas gingivalis2018

    • Author(s)
      Sakanaka A, Kuboniwa M, Ishikawa A, Mayumi S, Amano A
    • Organizer
      IADR 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Porphyromonas gingivalisの代謝産物シトルリンを介した栄養共生系の探索2018

    • Author(s)
      坂中哲人、久保庭雅恵、石川明日香、眞弓昌大、天野敦雄
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Nutritional Synergy Between Porphyromonas gingivalis and Filifactor alocis2018

    • Author(s)
      Kuboniwa M, Sakanaka A, Ishikawa A, Mayumi S, Lamont R, Amano A
    • Organizer
      IADR 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Dental biofilm microenvironment is potentially affected by nutritional synergy between Streptococcus gordonii and Porphyromonas gingivalis2018

    • Author(s)
      Kuboniwa M, Sakanaka A, Ishikawa A, Mayumi S, Amano A
    • Organizer
      Biofilm 8
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Thiamine plays a key role in the middle stage of oral biofilm formation2018

    • Author(s)
      Ishikawa A, Kuboniwa M, Sakanaka A, Mayumi S, Amano A
    • Organizer
      Asian Academy of Preventive Dentistry 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 口腔レンサ球菌Strptococcus oralisとStreptococcus gordoniiの抗酸化物質を介した共生関係2018

    • Author(s)
      眞弓昌大、久保庭雅恵、坂中哲人、石川明日香、天野敦雄
    • Organizer
      第29回近畿・中国・四国口腔衛生学会総会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2021-02-19  

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