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Synthesis of artificial cell-based incubators (microorganisms-containing artificial cells)

Research Project

Project/Area Number 18K18157
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 62010:Life, health and medical informatics-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

MORITA Masamune  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (90708504)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsリポソーム / 人工細胞 / 人工細胞型インキュベータ / 生細胞-人工細胞ハイブリッド / 微生物培養 / 人工細胞型インキュベーター / 人工生体システム / 人工細胞型リアクタ
Outline of Final Research Achievements

In this study, we present a new microorganism culture systems “artificial cell-based incubators”. The method is the first step toward culturing living cells inside artificial cell-like systems. By encapsulating microorganisms at the single-cell level inside liposomes (artificial cells) and observing the single liposomes (artificial cells) under an optical microscope, we succeeded in real-time monitoring of microbial growth. Furthermore, we succeeded in demonstrating its usefulness by conducting application experiments using liposomes (artificial cells) encapsulating microorganisms.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、リポソーム(人工細胞)内での微生物培養という、材料科学分野におけるリポソームのさらなる可能性や、生物工学・バイオ産業に貢献できる可能性を示すことができた。また、この成果は、微生物がホスト細胞に共生する「細胞内共生」という真核細胞の起源モデルにボトムアップ的手法によって迫り、新たな人工微生物(生命)の創生など生物学的テーマに貢献できる点で学術・社会的意義がある。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (15 results)

All 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Session 1SCA?Utilizing soft compartments/interfaces for the creation of artificial biosystems2020

    • Author(s)
      Sato Yusuke、Morita Masamune、Suzuki Yuki
    • Journal Title

      Biophysical Reviews

      Volume: 12 Issue: 2 Pages: 257-259

    • DOI

      10.1007/s12551-020-00647-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bacterial cell growth dynamics inside giant lipid vesicles for bottom-up synthetic biology2019

    • Author(s)
      Masamune Morita, Kaoru Katoh, Naohiro Noda
    • Journal Title

      Eur. Biophys. J. Biophy.

      Volume: 48 Pages: 208-208

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial Cell Culture at the Single-Cell Level Inside Giant Vesicles2019

    • Author(s)
      Masamune Morita, Yuri Ota, Kaoru Katoh, Naohiro Noda
    • Journal Title

      Journal of Visualized Experiments

      Volume: 146:e59555 Issue: 146 Pages: 1-7

    • DOI

      10.3791/59555

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a highthroughput screening method for environmental bacteria with various growth rates2019

    • Author(s)
      Yuri Ota, Kanako Saito, Taeko Takagi, Satoko Matsukura, Masamune Morita, Satoshi Tsuneda, Naohiro Noda
    • Journal Title

      Plos One

      Volume: 14(4):e0214533 Issue: 4 Pages: 1-8

    • DOI

      10.1371/journal.pone.0214533

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures2018

    • Author(s)
      Masamune Morita, Kaoru Katoh, Naohiro Noda
    • Journal Title

      ChemistryOpen

      Volume: 7 Issue: 11 Pages: 845-849

    • DOI

      10.1002/open.201800126

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Bacterial cell growth dynamics inside giant lipid vesicles for bottom-up synthetic biology2019

    • Author(s)
      Masamune Morita, Kaoru Katoh, Naohiro Noda
    • Organizer
      12th EBSA and 10th ICBP-IUPAP Biophysics congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Synthesis of artificial/living cell hybrid biosystems2019

    • Author(s)
      Masamune Morita, Kaoru Katoh, Naohiro Noda
    • Organizer
      第57回日本生物物理学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] リポソームを用いたシングルセルレベル培養技術の構築2019

    • Author(s)
      森田雅宗,加藤薫,野田尚宏
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Water-in-oilドロップレット内での環境微生物の培養とシングルドロップレットアイソレーション技術の開発2019

    • Author(s)
      斉藤加奈子,大田悠里, 松倉智子,髙木妙子,森田雅宗,常田聡,野田尚宏
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] RNase活性を指標とした微生物増殖water-in-oilドロップレットの検出法 “FNAP-sort” の確立2019

    • Author(s)
      大田悠里, 斉藤加奈子,松倉智子,髙木妙子,森田雅宗,常田聡,野田尚宏
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Water-in-oil ドロップレットを用いた腸内細菌培養手法の開発2019

    • Author(s)
      村山周平,斉藤加奈子,大田悠里, 松倉智子,森田雅宗,関口勇地,常田聡,野田尚宏
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] W/Oドロップレットを用いた環境微生物培養の基盤技術 “FNAP-sort” の開発2019

    • Author(s)
      大田悠里, 斉藤加奈子,松倉智子,髙木妙子,森田雅宗,常田聡,野田尚宏
    • Organizer
      環境バイオテクノロジー学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞内小器官型人工細胞を指向したジャイアントベシクル内微生物培養法の構築2019

    • Author(s)
      森田雅宗,加藤薫,野田尚宏
    • Organizer
      第2回分子ロボティクス年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞サイズリポソーム(人工細胞)内での微生物培養2018

    • Author(s)
      森田雅宗,加藤薫,野田尚宏
    • Organizer
      「細胞を創る」研究会11.0
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 微生物を封入したベシクルを用いた微生物の培養方法2018

    • Inventor(s)
      森田雅宗,野田尚宏,髙木妙子,大田悠里, 斉藤加奈子
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-089417
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2021-02-19  

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