The effects of ionizing radiation on early development stage of the fertilized egg
Project/Area Number |
18K18196
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 63020:Radiation influence-related
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Research Institution | Hiroshima University |
Principal Investigator |
Kanai Akinori 広島大学, 原爆放射線医科学研究所, 助教 (60549567)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 放射線 / 受精卵 / 次世代シーケンサー / バイオインフォマティクス |
Outline of Final Research Achievements |
In this study, we analyzed the effects of low-dose radiation on early implantation embryos using fertilized mouse eggs. In the case of mouse fertilized eggs, the greatest decrease in development was observed at 5 hours after fertilization, confirming the high sensitivity to radiation. When the fertilized eggs were irradiated with 0.1 Gy, only developmental arrest at the morula stage was observed, but when the eggs were irradiated with 1 Gy, two stages of developmental arrest were observed: one at the 1-4 cell stage and the other at the morula stage. Transcriptome analysis of the morula embryos suggested that the same mechanism of developmental arrest at the morula stage was observed at 0.1 and 1 Gy.
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Academic Significance and Societal Importance of the Research Achievements |
妊娠期における放射線被曝影響は、胎芽・胎児の発育期によって著しく異なることが明らかにされているが、妊娠期における低線量放射線が与える影響に関しては未だ不明な点が多い。本研究によって、マウス受精卵は媒精後5時間の段階で放射線の影響を最も受けることを確認し、線量の違いによって発生停止段階が異なることも確認した。これらのことはヒト受精卵においても同様に放射線の影響を受ける事が予想される。 また、受精卵への影響の分子機構を調べるために遺伝子解析も行うことで受精卵の発生停止に影響する候補のひとつとしてTrim43遺伝子群を見出し、今後詳細な解析を進めるつもりである。
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] UTX maintains the functional integrity of the murine hematopoietic system by globally regulating aging-associated genes2021
Author(s)
Sera Y, Nakata Y, Ueda T, Yamasaki N, Koide S, Kobayashi H, Ikeda KI, Kobatake K, Iwasaki M, Oda H, Wolff L, Kanai A, Nagamachi A, Inaba T, Sotomaru Y, Ichinohe T, Koizumi M, Miyakawa Y, Honda ZI, Iwama A, Suda T, Takubo K, Honda H
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Journal Title
Blood
Volume: 137
Pages: 908~922
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] HHEX promotes myeloid transformation in cooperation with mutant ASXL12020
Author(s)
Takeda R, Asada S, Park SJ, Yokoyama A, Becker HJ, Kanai A, Visconte V, Hershberger C, Hayashi Y, Yonezawa T, Tamura M, Fukushima T, Tanaka Y, Fukuyama T, Matsumoto A, Yamasaki S, Nakai K, Yamazaki S, Inaba T, Shibata T, Inoue D, Honda H, Goyama S, Maciejewski JP, Kitamura T
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Journal Title
Blood
Volume: 136
Pages: 1670~1684
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Kdm6a Deficiency Activates Inflammatory Pathways, Promotes M2 Macrophage Polarization, and Causes Bladder Cancer in Cooperation with p53 Dysfunction2020
Author(s)
Kobatake K, Ikeda KI, Nakata Y, Yamasaki N, Ueda T, Kanai A, Sentani K, Sera Y, Hayashi T, Koizumi M, Miyakawa Y, Inaba T, Sotomaru Y, Kaminuma O, Ichinohe T, Honda ZI, Yasui W, Horie S, Black PC, Matsubara A, Honda H
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Journal Title
Clinical Cancer Research
Volume: 26
Pages: 2065~2079
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A cell-based high-throughput screening method to directly examine transthyretin amyloid fibril formation at neutral pH2019
Author(s)
Ueda M, Okada M, Mizuguchi M, Kluve-Beckerman B, Kanenawa K, Isoguchi A, Misumi Y, Tasaki M, Ueda A, Kanai A, Sasaki R, Masuda T, Inoue Y, Nomura T, Shinriki S, Shuto T, Kai H, Yamashita T, Matsui H, Benson MD, Ando Y
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Journal Title
Journal of Biological Chemistry
Volume: 294
Pages: 11259~11275
DOI
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Peer Reviewed / Int'l Joint Research
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[Journal Article] A novel ASXL1-OGT axis plays roles in H3K4 methylation and tumor suppression in myeloid malignancies2018
Author(s)
Inoue D, Fujino T, Sheridan P, Zhang YZ, Nagase R, Horikawa S, Li Z, Matsui H, Kanai A, Saika M, Yamaguchi R, Kozuka-Hata H, Kawabata KC, Yokoyama A, Goyama S, Inaba T, Imoto S, Miyano S, Xu M, Yang FC, Oyama M, Kitamura T.
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Journal Title
Leukemia
Volume: 32
Pages: 1327~1337
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation2018
Author(s)
Nagase R, Inoue D, Pastore A, Fujino T, Hou HA, Yamasaki N, Goyama S, Saika M, Kanai A, Sera Y, Horikawa S, Ota Y, Asada S, Hayashi Y, Kawabata KC, Takeda R, Tien HF, Honda H, Abdel-Wahab O, Kitamura T.
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Journal Title
The Journal of Experimental Medicine
Volume: 215
Pages: 1729~1747
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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