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Development of a microchip for extracellular vesicle naked-eye detection and easy analysis toward point-of-care diagnosis

Research Project

Project/Area Number 18K18404
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 90130:Medical systems-related
Research InstitutionJuntendo University

Principal Investigator

ISHIHARA Ryo  順天堂大学, 医学部, 助教 (30633507)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords表面機能化自律駆動マイクロチップ / その場診断 / バイオマーカー / 胞外ベシクル / 乳がん / UVグラフト重合 / 血清 / UVグラフト重合法 / 細胞外ベシクル / 複数同時検出 / 裸眼検出 / 簡易解析
Outline of Final Research Achievements

This study aims to develop a microchip that can diagnose cancer by point-of-care like a pregnancy test. Extracellular vesicles (EVs) have been attracted as a biomarker for cancer detection at early stage, but EV-based cancer point-of-care diagnosis has not been realized because the detection technique requires complicated operations, long detection time, and high cost. We have developed the surface-functionalized power-free microchip for EV detection and succeeded in EV detection of several hundred times higher concentration than in vivo. In this grant period, the microchip was further developed, and the following accomplishments were obtained. (1) Various knowledge in the microchip preparation was obtained. (2) We succeeded in detecting EV with high sensitivity. The grant period was extended, and (3) not only in physiological saline but also in serum EV was successfully detected on the microchip.

Academic Significance and Societal Importance of the Research Achievements

本申請では,より実用化へ近づけるために,申請者の開発した表面機能化自律駆動マイクロチップを用いた細胞外ベシクル(EV)の検出を“裸眼”検出へと発展させることをめざした。また,細胞外ベシクルの解析手法は現在未確立であり,ラボレベルで提案されている手法は煩雑であることから,簡易解析も同時にめざした。これらは実現すれば,新規の手法・材料ということで分析化学・材料工学的な価値,疾患の早期発見という医学的な価値に加え,EVを誰もが容易に扱えるようになるためEV研究を前進させることが出来るという観点から生物学的な価値もあると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (19 results)

All 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (15 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Design of a surface-functionalized power-free microchip for extracellular vesicle detection utilizing UV grafting2019

    • Author(s)
      Ishihara R.、Katagiri A.、Nakajima T.、Matsui R.、Komatsu S.、Hosokawa K.、Maeda M.、Tomooka Y.、Kikuchi A.
    • Journal Title

      Reactive and Functional Polymers

      Volume: 142 Pages: 183-188

    • DOI

      10.1016/j.reactfunctpolym.2019.06.017

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Preparation of Pt/WO3-Coated Polydimethlsiloxane Membrane for Transparent/Flexible Hydrogen Gas Sensors2019

    • Author(s)
      R. Ishihara, Y. Yamaguchi, K. Tanabe, Y. Makino, K. Nishio
    • Journal Title

      Materials Chemistry and Physics

      Volume: 226 Pages: 226-229

    • DOI

      10.1016/j.matchemphys.2018.12.052

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Design of Capture Materials Utilizing Radiation-Induced Graft Polymerization2018

    • Author(s)
      石原 量
    • Journal Title

      KOBUNSHI RONBUNSHU

      Volume: 75 Issue: 5 Pages: 456-467

    • DOI

      10.1295/koron.2018-0015

    • NAID

      130007490328

    • ISSN
      0386-2186, 1881-5685
    • Year and Date
      2018-09-25
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Fabrication of Pt/WO3-Polydimethylsiloxane Hybrid Membrane for Hydrogen Gas Sensor2020

    • Author(s)
      R. Ishihara,Y. Yamaguchi, K. Tanabe, Y. Makino, K. Nishio
    • Organizer
      第69回高分子学会年次大会 (予稿集での発表)
    • Related Report
      2020 Annual Research Report
  • [Presentation] バイオマーカーの高感度簡易検出を可能とする表面機能化自律駆動マイクロチップの開発2020

    • Author(s)
      石原 量
    • Organizer
      千葉大学工学部講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] がんのその場診断をめざした表面機能化自律駆動マイクロチップの開発2020

    • Author(s)
      石原 量
    • Organizer
      順天堂大学浦安病院 TVカンファレンス
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Design of a Microchip for Cancer Cell-Derived Extracellular Vesicle Detection toward Cancer Point-of-Care Diagnosis2019

    • Author(s)
      R. Matsui, R. Ishihara, A. Katagiri, T. Nakajima, K. Hosokawa, M. Maeda, A. Kikuchi
    • Organizer
      2nd Glowing Polymer Symposium in KANTO (GPS-K2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a Surface-Functionalized Power-Free Microchip for microRNAs Multiplex Detection2019

    • Author(s)
      R. Kitane, R. Ishihara, Y. Akiyama, K. Hosokawa, M. Maeda, A. Kikuchi
    • Organizer
      2nd Glowing Polymer Symposium in KANTO (GPS-K2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Pt/WO3-Coated Polydimethylsiloxane Membrane for Transparent/Flexible Hydrogen Gas Monitoring2019

    • Author(s)
      R. Ishihara, Y. Yamaguchi, K. Tanabe, Y. Makino, K. Nishio
    • Organizer
      2019 MRS Fall Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] がん細胞由来細胞外ベイクルの検出をめざしたマイクロチップの作製2019

    • Author(s)
      松井崚,石原量,中島忠章,片桐明日香, 細川和生, 前田瑞夫,友岡康弘, 菊池明彦
    • Organizer
      第36回高分子学会千葉地域活動若手セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] がんのその場検査のためのmicroRNA複数同時検出用マイクロチップの作製2019

    • Author(s)
      喜種椋一,石原量,秋山好嗣,猪股祥子, 細川和生, 前田瑞夫, 菊池明彦
    • Organizer
      第36回高分子学会千葉地域活動若手セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞外ベシクル高感度検出のための表面機能化自律駆動マイクロチップの設計2018

    • Author(s)
      石原 量, 片桐明日香,中島忠章,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第67回高分子学会年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Design of a Microchip for Extracellular Vesicle Detection toward Point-of-Care Testing2018

    • Author(s)
      R. Ishihara, T. Nakajima, R. Matsui, A. Katagiri, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
    • Organizer
      1st Glowing Polymer Symposium in KANTO
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] がん特異的な細胞外ベシクルの検出をめざしたマイクロチップの作製2018

    • Author(s)
      松井崚,石原 量, 片桐明日香,中島忠章,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第67回高分子学会年次大会,愛知・名古屋国際会議場
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞外ベシクルの簡易・高感度検出のためのマイクロチップおよび検出プロトコルの検討2018

    • Author(s)
      片桐明日香, 石原量, 中島忠章,細川和生, 前田瑞夫, 友岡康弘, 菊池明彦
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 表面機能化自律駆動マイクロチップを用いたがん細胞由来細胞外ベシクルの検出2018

    • Author(s)
      松井崚,石原 量, 中島忠章,片桐明日香,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 表面機能化自律駆動マイクロチップを用いたmicroRNAの複数同時検出2018

    • Author(s)
      喜種椋一,石原 量,秋山好嗣,猪股祥子,細川和生, 前田瑞夫,菊池明彦
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Effect of MicroRNA Supply to Functionalized Surface of Power-free Microchip on Detection Signal Intensity2018

    • Author(s)
      S. INOMATA, R. ISHIHARA, K. HOSOKAWA, M. MAEDA, and A. KIKUCHI
    • Organizer
      第28回日本MRS年次大会
    • Related Report
      2018 Research-status Report
  • [Book] 「早期発見・予防に向けた次世代がん検査技術の最前線」 Frontier of Next-generation Cancer Testing Technology for Detection and Response2019

    • Author(s)
      石原量,中島忠章 他31名
    • Total Pages
      216
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314051
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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