Profiling of multidrug-resistant E. coli in urban sewage in Asia and Africa by using whole-genome sequencing
| Project/Area Number |
18K18881
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 22:Civil engineering and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
Harada Hidenori 京都大学, アジア・アフリカ地域研究研究科, 准教授 (40512835)
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| Co-Investigator(Kenkyū-buntansha) |
五味 良太 京都大学, 工学研究科, 助教 (30794284)
松田 知成 京都大学, 工学研究科, 准教授 (50273488)
松村 康史 京都大学, 医学研究科, 准教授 (80726828)
山本 正樹 京都大学, 医学研究科, 講師 (90726837)
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| Project Period (FY) |
2018-06-29 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 多剤耐性大腸菌 / 都市下水 / アジア・アフリカ / 全ゲノム解析 |
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| Outline of Final Research Achievements |
The global increase of antimicrobial resistance (AMR) is a major public health concern. Our study in Kampala, Uganda revealed the prevalence and transmission of AMR E. coli including high-risk clones, demonstrating that a combination of sewage (or fecal sludge) surveillance and whole-genome sequencing can be a powerful tool for monitoring AMR carriage in the underlying population. Also, our study in Bangkok, Thailand showed that large amounts of AMR E. coli were released into the environment even after passing through sewage treatment plants, and that the concentration of AMR E. coli increased in correlation with population density. These results indicated that substantial release and transmission of AMR E. coli in the environment in the target area.
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| Academic Significance and Societal Importance of the Research Achievements |
途上国由来の薬剤耐性菌の広がりが世界的に懸念されている。本研究では東南アジアではタイのバンコク，アフリカではウガンダのカンパラを対象に，都市レベルでの薬剤耐性大腸菌の広がりを下水調査により明らかにした。タイでは使用する抗菌薬に応じた耐性大腸菌がもっとも多くみられるとともに，人口密度に応じてその濃度も高まることがわかった。ウガンダでは，薬剤耐性大腸菌の全ゲノム解析から，世界的な高リスククローンが検出されたとともに，その耐性を決定する多様な因子が確認された。また，下水調査と全ゲノム解析の組み合わせが都市レベルの薬剤耐性の広がりを把握する有効なツールとなることを示した。
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Report
(6 results)
Research Products
(30 results)
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[Journal Article] Fecal contamination and the proportion of human-associated E.coli along Nhue river, Viet Nam2018
Author(s)
Mai Tanaka, Hidenori Harada, Shigeo Fujii, Min Li Chua, Nguyen Duy, Hung, Nguyen Pham Hong Lien, Nghiem Trung Dung, Ryota Gomi
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Journal Title
Vietnam Journal of Science and Technology,
Volume: 56(2C)
Pages: 23-29
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Cross-country performance of a human associated E. coli source tracking marker, H8, in Asia and Africa2019
Author(s)
Hidenori Harada, Chua Min Li, Mai Tanaka, Nguyen Pham Hong Lien, Allan J Komakech, Nazmul Ahsan, Meki Chirwa, Imasiku Nyambe, Ryota Gomi, Shigeo Fujii
Organizer
20th Symposium on Health-Related Water Microbiology
Related Report
Int'l Joint Research
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[Presentation] Proportions of human-associated Escherichia coli isolates along a heavily contaminated river in Vietnam2018
Author(s)
Harada, H., Tanaka, M., Fujii, S., Chua, M.L., Nguyen, D.H., Nguyen, P.H.L., Nghiem T.D., Gomi, R.
Organizer
Water Microbiology 2018
Related Report
Int'l Joint Research
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[Book] サニテーションと健康2023
Author(s)
原田 英典、山内 太郎
Total Pages
230
Publisher
北海道大学出版会
ISBN
9784832929548
Related Report
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