| Project/Area Number |
18K19054
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 32:Physical chemistry, functional solid state chemistry, and related fields
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 蛍光イメージ / １分子観察 / 一分子観察 |
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| Outline of Final Research Achievements |
In 2017, we developed cryogenic fluorescence microscope equipped with off-focusing imaging system. The microscope have localized individual fluorophores with angstrom spatial precision. However, the off-focusing imaging is not suitable for a cellular imaging because of a strong autofluorescence of cells. In the present project, we have developed a new cryogenic fluorescence microscope equipped with mutifocus imaging system. We demonstrated the mutifocus imaging of an individual fluorophore. The three dimensional position was localized with 1 nm precision. The experimental precision is 1.5 times of the theoretical limit.
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| Academic Significance and Societal Importance of the Research Achievements |
顕微鏡が開発されてから４００年あまり、様々な顕微鏡が提案されてきた。しかし、細胞内部を分子レベルで見ることができる顕微鏡はいまだない。現在、医学薬学の分野でも分子レベルの研究が進んでいるが、実は最も大事な可視化技術が抜け落ちている。このままでは、生命現象の理解や疾患の克服などを実現することはとても困難である。本課題は、この人類の課題であった「細胞内分子レベルイメージング」を実現するための鍵となる技術であり、学術的にも社会的意義が高いと考えている。
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