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Construction of Artificial Phycobilisome assisted by DNA scaffold

Research Project

Project/Area Number 18K19145
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 37:Biomolecular chemistry and related fields
Research InstitutionKyoto University

Principal Investigator

NAKATA EIJI  京都大学, エネルギー理工学研究所, 准教授 (70467827)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsヘテロ多量体 / DNAナノ構造体 / DNA結合タンパク質 / 平衡制御 / フィコビリソーム
Outline of Final Research Achievements

DNA nanostructure was used as the scaffold to locate the functional molecules including proteins and enzymes. We recently developed the method "DNA binding adaptor" to locate protein and enzyme on the DNA scaffold. In this research, we would like to apply the method to construct the protein assembled state for appearing the new function. As a function, we would like to construct artificial phycobilisome by assembling the phycobiliprotein. Through this research, we developed the new adaptor series to assemble various proteins.

Academic Significance and Societal Importance of the Research Achievements

タンパク質は高機能な生体高分子であり、この機能を自在に制御して温和な条件下で様々なシステムを構築して利用することは持続可能な社会において必要とされる技術です。本研究では、タンパク質を自在に配置することが可能なDNAナノ構造体を足場として、我々が開発したタンパク質配置技術を利用して高機能なシステムを構築することを目指しています。そのひとつとして、タンパク質本来の光捕集能を操作できる可能性について検討をおこなうのがこの研究の目的でしたが、これに限らず様々な応用展開が期待される拡張性の高い研究手法です。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (27 results)

All 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (22 results) (of which Int'l Joint Research: 9 results,  Invited: 7 results)

  • [Journal Article] Rational design of a DNA sequence specific modular protein tag by tuning the alkylation kinetics2019

    • Author(s)
      T.M. Nguyen, E. Nakata, Z. Zhang, M. Saimura, H. Dinh, T. Morii
    • Journal Title

      Chemical Science

      Volume: 10 Issue: 40 Pages: 9315-9325

    • DOI

      10.1039/c9sc02990g

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reaction of ribulose biphosphate carboxylase/oxygenase assembled on a DNA scaffold2019

    • Author(s)
      H. Dinh, E. Nakata, P. Lin, M. Saimura, H. Ashida, T. Morii
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 27 Issue: 22 Pages: 115120-115120

    • DOI

      10.1016/j.bmc.2019.115120

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein adaptors assemble functional proteins on DNA scaffolds2019

    • Author(s)
      T.D. Ngo, Tien, N. Huyen, T.M. Nguyen, F.F. Liew, E. Nakata, T. Morii
    • Journal Title

      Chemical Communications

      Volume: 27 Issue: 83 Pages: 12428-12446

    • DOI

      10.1039/c9cc04661e

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA binding adaptors to assemble proteins of interest on DNA scaffold2019

    • Author(s)
      Nakata Eiji、Dinh Huyen、Nguyen Thang Minh、Morii Takashi
    • Journal Title

      Methods Enzymol.

      Volume: 617 Pages: 287-322

    • DOI

      10.1016/bs.mie.2018.12.014

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Covalent Bond Formation by Modular Adaptors to Locate Multiple Enzymes on a DNA Scaffold2019

    • Author(s)
      Nakata, E.; Nakano, S.; Rajendran, A.; Morii, T.
    • Journal Title

      Kinetic Control in Synthesis and Self-Assembly 2019, pp.163-183

      Volume: 1 Pages: 163-183

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Tuning the reactivity of crosslink formation for sequence-selective DNA-protein conjugation2020

    • Author(s)
      Z. ZHANG, E. NAKATA, N. THANG, M. SAIMURA, K. MATSUDA, T. MORII
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Enhanced enzymatic activity with a packed assembly on DNA scaffold2020

    • Author(s)
      H. DINH, E. NAKATA, K. MUTSUDAZAPATER, M. SAIMURA, M. KINOSHITA, T. MORII
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] A dynamic DNA scaffold for enzyme cascade reaction2020

    • Author(s)
      P. LIN, H. DINH, Y. MORITA, E. NAKATA, T. MORII
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 酵素間距離を制御する分子コンビナートを用いた非天然化合物合成システムの創製2019

    • Author(s)
      中田栄司,森井孝
    • Organizer
      新学術領域「生合成リデザイン」公開シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 反応速度論に基づいたDNA配列選択的なモジュール型アダプターの設計2019

    • Author(s)
      中田栄司,張正宵,Nguyen Minh Thang,才村正幸,松田一成,森井孝
    • Organizer
      第13回バイオ関連化学シンポジウム2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA scaffold2019

    • Author(s)
      E. Nakata, T. Morii
    • Organizer
      第10回日英シンポジウム
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design of sequence specific modular adaptors by tuning the reactivity of protein-tag substrate2019

    • Author(s)
      Zhengxiao Zhang, Eiji Nakata, Thang Minh Nguyen, Takashi Morii
    • Organizer
      ISNAC2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on a DNA scaffold2019

    • Author(s)
      E. Nakata, T. Morii
    • Organizer
      The 2nd International Symposium of Biofunctional Chemistry
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on a DNA scaffold2019

    • Author(s)
      E. Nakata, T. Morii
    • Organizer
      曁南大学 講義
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on a DNA scaffold2019

    • Author(s)
      E. Nakata, T. Morii
    • Organizer
      武漢大学 講義
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 酵素を“1分子ずつ”狙った場所に配置する―生物から学んだ分子コンビナートへの挑戦―2019

    • Author(s)
      中田栄司・森井 孝
    • Organizer
      東京農業大学
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Tuning the reactivity of crosslink formation for sequence-selective DNA-protein conjugation2019

    • Author(s)
      ZHANG, Zhengxiao; NAKATA, Eiji; THANG, Nguyen Minh; MORII, Takashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Enhanced enzymatic activity with a packed assembling of enzyme2019

    • Author(s)
      DINH, Huyen; NAKATA, Eiji; KINOSHITA, Masahiro; MORII, Takashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Reaction of enzyme cascades assembled in 3D DNA nanostructure2019

    • Author(s)
      LIN, Peng; DINH, Huyen; THANG, Minh Nguyen; NAKATA, Eiji; MORII, Takashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA scaffold2018

    • Author(s)
      Eiji Nakata, Takashi Morii
    • Organizer
      理研・CSRS融合研究セミナー
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] DNAナノ構造体上に複数種類の酵素を配置した分子コンビナート2018

    • Author(s)
      中田栄司・森井 孝
    • Organizer
      東京大学薬学研究科天然物化学教室 講演会#121
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Construction of a 3D DNA nanostructure for assembling enzyme cascade reaction2018

    • Author(s)
      LIN Peng・Dinh Huyen・ Thang Minh Nguyen・Nakata Eiji・Morii Takashi
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA scaffold2018

    • Author(s)
      Eiji Nakata, Takashi Morii
    • Organizer
      ACeND 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] DNA Binding Adaptors for Locating Multiple Enzymes on DNA scaffold2018

    • Author(s)
      Eiji Nakata, Thang Minh Nguyen, Zhengxiao Zhang, Huyen Dinh, Peng Lin, Masayuki Saimura, Takashi Morii
    • Organizer
      ISNAC2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Assembling cascade enzymes on a 3D DNA nanostructure2018

    • Author(s)
      Peng LIN, Huyen Dinh, Nguyen Minh Thang, Eiji Nakata, Takashi Morii
    • Organizer
      ISNAC2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Design of sequence specific modular adaptors by tuning the reactivity of protein-tag substrates2018

    • Author(s)
      Zhengxiao Zhang, Eiji Nakata, Thang Minh Nguyen, Takashi Morii
    • Organizer
      ISNAC2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] DNA Binding Adaptors to Locate Multiple Enzymes on DNA scaffold2018

    • Author(s)
      Eiji Nakata, Takashi Morii
    • Organizer
      ISNM2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-07-25   Modified: 2023-01-30  

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