Research on microdomain visualization probes to elucidate plant immunity
| Project/Area Number |
18K19164
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
長野 稔 立命館大学, 生命科学部, 助教 (80598251)
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| Project Period (FY) |
2018-06-29 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マイクロドメイン / ステロール / 可視化プローブ / 脂質ラフト / スフィンゴ脂質 / グリコシルイノシトールホスホセラミド / 植物免疫 |
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| Outline of Final Research Achievements |
In order to examine the functions of microdomains scattered on the plasma membrane, which play an important role in plant disease response, we have developed plant microdomain visualization probes targeting their major constituent lipids. We developed a visualization probe, GFP-D4L, which binds to phytosterols, and succeeded in visualizing microdomain structures on the plasma membrane. We also generated an Arabidopsis line that constitutively expresses GFP-D4L and showed that the microdomain exhibits specific dynamics during disease response.
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| Academic Significance and Societal Importance of the Research Achievements |
毎年、世界の作物の約15%が病害によって失われている。原因となる病原体と植物細胞の接点は細胞膜であるため、細胞膜には多数の免疫タンパク質が存在する。免疫タンパク質の多くは細胞膜マイクロドメインに局在するため、植物のマイクロドメインの機能解明は重要である。本研究では、植物マイクロドメインの可視化プローブを開発し、病害応答時におけるマイクロドメインの動態を解明した。マイクロドメイン機能の理解は、将来的にマイクロドメインの改変による耐病性を強化した作物の作出に繋がると期待できる。
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Report
(5 results)
Research Products
(35 results)
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[Journal Article] Suppressing Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan II2018
Author(s)
Sechet J, Htwe S, Urbanowicz B, Agyeman A, Feng W, Ishikawa T, Dinneny J, Colomes M, Satish KK, Kawai-Yamada M, O'Neill M, Mortimer J
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Journal Title
The Plant Journal
Volume: 96
Issue: 5
Pages: 1036-1050
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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