All-round peroxidase contribute the auxin catabolism and regulates plant growth and morphology

• Project/Area Number: 18K19234
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
• Research Institution: Kyushu University
• Principal Investigator: Tsutsumi Yuji 九州大学, 農学研究院, 教授 (30236921)
• Co-Investigator(Kenkyū-buntansha): 雉子谷 佳男 宮崎大学, 農学部, 教授 (10295199)
• Project Period (FY): 2018-06-29 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ペルオキシダーゼ / オーキシン / 成長・分化 / リグニン / CWPO-C / 植物ホルモン / 木化 / 基質万能性 / 形態形成 / 植物ホルモン代謝 / 分化・成長

Outline of Final Research Achievements

The over-expression of CWPO-C (OE), a unique all-round peroxidase, in Arabidopsis were analyzed. The OE mutants showed delay of growth and gravitropism. These phenotype changes are similar to the IAA deficient phenotype. IAA contents in OE mutants were largely decreased compared to wild type. The results strongly suggest that CWPO-C catabolizes and inactivates IAA in vivo. This hypothesis was further supported by the in vitro conversion of IAA by recombinant CWPO-C. IAA was converted into mainly into two unknown compounds but not OxIAA, which are catabolite by IAA oxidase. These finding may give a new insight of IAA catabolism in plant.

Academic Significance and Societal Importance of the Research Achievements

IAAの代謝不活化機構については、未解明の部分が残されておりIAAオキシダーゼによるOxIAAへの代謝変換やアミノ酸抱合体やグルコース抱合体への変換などが提案されている。一方本研究において、万能ペルオキシダーゼCWPO-C過剰発現体の表現型は、IAA欠乏状態の表現型とよく一致し、内生IAA量が著しく低下していたこと、組換体CWPO-CによるIAA代謝が確認されたことからCWPO-Cによる新たなIAA代謝経路が示唆された。IAAは植物の成長や分岐を制御する重要な因子であり、生産性と価値に大きな影響を持つため、その作用メカニズム解明は植物生産において非常に重要な知見になりうる。

Research Products

• [Presentation] 万能ペルオキシダーゼ、 CWPO-Cによる植物の成長制御とオーキシン代謝 (2020)
  • Author(s): 横山 裕亮、富家 梓、YOSHIKAY Diego、雉子谷 佳男、堤 祐司
  • Organizer: 第70回日本木材学会大会
• [Presentation] Regulation of differentiation and growth by plant peroxidase CWPO-C (2019)
  • Author(s): Diego A. Yoshikay, Yusuke Yokoyama , Jun Shigeto, Yuji Tsutsumi
  • Organizer: 1st International Lignin Symposium
  • Int'l Joint Research
• [Presentation] 植物ペルオキシダーゼ、CWPO-C による成長制御作用機序の解明 (2019)
  • Author(s): 横山 裕亮、富家 梓、YOSHIKAY Diego、雉子谷 佳男、堤 祐司
  • Organizer: 第 26 回日本木材学会九州支部大会
• [Presentation] 植物ペルオキシダーゼ、CWPO-Cによる分化成長の制御 (2019)
  • Author(s): 横山 裕亮、重藤 潤、大平 香織、堤 祐司
  • Organizer: 第69回日本木材学会大会
• [Presentation] The contribution of CWPO-C to primary stage of plant growth and organogenesis (2018)
  • Author(s): Diego Yoshikay, Jun Shigeto, Yusuke Yokoyama, Yuji Tsutsumi
  • Organizer: 5th Symposium of Biotechnology Applied to Lignocelluloses
  • Int'l Joint Research
• [Presentation] Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and sinapyl alcohol via plant peroxydase involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis (2018)
  • Author(s): Yuji Tsutsumi, Jun Shigeto, Hiroki Honjo
  • Organizer: 29th International Conference of Polyphenols,
  • Int'l Joint Research