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The influence of cervine piroplasma on the onset of bovine piroplasmosis

Research Project

Project/Area Number 18K19257
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 42:Veterinary medical science, animal science, and related fields
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

Yokoyama Naoaki  帯広畜産大学, 原虫病研究センター, 教授 (80301802)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsシカ / タイレリア / バベシア / マダニ / 牛 / 感染 / リスク / シカ小型ピロプラズマ / シカ大型ピロプラズマ / 牛小型ピロプラズマ / LAMP法 / 制御法 / 貧血 / 放牧 / 小型ピロプラズマ / 牛小型ピロプラズマ病
Outline of Final Research Achievements

We demonstrated for the first time that wild deer in Hokkaido are infected with a Theileria species designated Theileria sp. (sika 1) and two Babesia species designated Babesia sp. (sika 1) and Babesia sp. (sika 2). Our findings also suggested that Ixodes persulcatus and Haemaphysalis longicornis, which are widespread in Hokkaido, are potential transmission vectors of these cervine Theileria and Babesia species. Interestingly, these parasite species were not detected in cattle from the same regions where the infected deer were found. In conclusion, our findings suggest that the risk of being infected with cervine Babesia and Theileria species is very low in cattle.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、野生動物に寄生する新たなピロプラズマ原虫の存在と生活環の一部が学術的に明らかとなった。また、それら原虫を迅速に検出できるPCR法やLAMP法など新たな診断技術の開発にも成功した。一方で、“シカ小型ピロプラズマを活用した牛小型ピロプラズマ病に対する新たな制御法”を考案するには至らなかった。しかし、マダニへの人工感染系が確立できたことから、牛小型ピロプラズマのライフサイクルの詳細な検証が可能となり、今後牛小型ピロプラズマ病に対するワクチンの開発研究が加速するものと期待する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (17 results)

All 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (1 results) Remarks (3 results)

  • [Int'l Joint Research] University of Joseph Ki-Zerbo(ブルキナファソ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Joseph Ki-Zerb(ブルキナファソ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] 国立獣医学研究所(スリランカ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of Rajshahi,(バングラデシュ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] North West University(南アフリカ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] スリランカ獣医学研究所(スリランカ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Development and evaluation of a novel loop-mediated isothermal amplification (LAMP) method targeting Theileria parasites infecting Yezo sika deer2020

    • Author(s)
      Shirozu Takahiro、Badolo Athanase、Soga Akira、Yoshimura Aya、Morishita Yu-ki、Koketsu Mami、Shirafuji Rika-Umemiya、Inokuma Hisashi、Yokoyama Naoaki、Fukumoto Shinya
    • Journal Title

      Parasitology International

      Volume: 77 Pages: 102130-102130

    • DOI

      10.1016/j.parint.2020.102130

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Discovery of a new Theileria sp. closely related to Theileria annulata in cattle from Sri Lanka2019

    • Author(s)
      Sivakumar Thillaiampalam、Fujita Shiori、Tuvshintulga Bumduuren、Kothalawala Hemal、Silva Seekkuge Susil Priyantha、Yokoyama Naoaki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41598-019-52512-y

    • NAID

      120006769784

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First Molecular detection of <i>Theileria annulata</i> in Bangladesh2019

    • Author(s)
      MONI Mst. Ishrat Zerin、HAYASHI Kei、SIVAKUMAR Thillaiapalam、RAHMAN Moizur、NAHAR Lovely、ISLAM Md. Zakirul、YOKOYAMA Naoaki、KITOH Katsuya、APPIAH-KWARTENG Cornelia、TAKASHIMA Yasuhiro
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 8 Pages: 1197-1200

    • DOI

      10.1292/jvms.19-0220

    • NAID

      130007695235

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Review on Equine Piroplasmosis: Epidemiology, Vector Ecology, Risk Factors, Host Immunity, Diagnosis and Control2019

    • Author(s)
      Onyiche ThankGod E.、Suganuma Keisuke、Igarashi Ikuo、Yokoyama Naoaki、Xuan Xuenan、Thekisoe Oriel
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 16 Issue: 10 Pages: 1736-1736

    • DOI

      10.3390/ijerph16101736

    • NAID

      120006708986

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epidemiological survey of hemoprotozoan parasites in cattle from low-country wet zone in Sri Lanka2019

    • Author(s)
      Gunasekara Erandi、Sivakumar Thillaiampalam、Kothalawala Hemal、Abeysekera Thuduwege Sanath、Weerasingha Amitha Sampath、Vimalakumar Singarayar Caniciyas、Kanagaratnam Ratnam、Yapa Palitha Rohana、Zhyldyz Atambekova、Igarashi Ikuo、Silva Seekkuge Susil Priyantha、Yokoyama Naoaki
    • Journal Title

      Parasitology International

      Volume: 71 Pages: 5-10

    • DOI

      10.1016/j.parint.2019.03.004

    • NAID

      120006603077

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Epidemiological survey of a cervine Theileria in wild deer, questing ticks, and cattle in Hokkaido, Japan2018

    • Author(s)
      Shibata Shunya、Sivakumar Thillaiampalam、Igarashi Ikuo、Umemiya-Shirafuji Rika、Inokuma Hisashi、Fukumoto Shinya、Yokoyama Naoaki
    • Journal Title

      Ticks and Tick-borne Diseases

      Volume: 9 Issue: 5 Pages: 1235-1240

    • DOI

      10.1016/j.ttbdis.2018.05.006

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of a mouse-tick infection model for Theileria orientalis and analysis of its transcriptome2018

    • Author(s)
      Hayashida Kyoko、Umemiya-Shirafuji Rika、Sivakumar Thillaiampalam、Yamagishi Junya、Suzuki Yutaka、Sugimoto Chihiro、Yokoyama Naoaki
    • Journal Title

      International Journal for Parasitology

      Volume: 48 Issue: 12 Pages: 915-924

    • DOI

      10.1016/j.ijpara.2018.05.012

    • NAID

      120006533722

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 北海道の野生シカ、生息マダニ、及び放牧牛におけるシカタイレリアの感染疫学調査2018

    • Author(s)
      横山直明,柴田峻也,Thillaiampalam Sivakumar,五十嵐郁男,白藤梨可,猪熊 壽,福本晋也
    • Organizer
      第70回 日本衛生動物学会大会
    • Related Report
      2018 Research-status Report
  • [Remarks] 帯広畜産大学・原虫用研究センター

    • URL

      https://www.obihiro.ac.jp/facility/protozoa/

    • Related Report
      2020 Annual Research Report
  • [Remarks] OIE Reference Centres

    • URL

      https://www.obihiro.ac.jp/facility/protozoa/en/oie-reference-centres

    • Related Report
      2020 Annual Research Report
  • [Remarks] 帯広畜産大学・原虫病研究センター

    • URL

      https://www.obihiro.ac.jp/facility/protozoa/

    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-07-25   Modified: 2022-01-27  

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