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Studies on the missing link between stay green and adaptation to environment

Research Project

Project/Area Number 18K19343
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionOkayama University

Principal Investigator

Sakamoto Wataru  岡山大学, 資源植物科学研究所, 教授 (20222002)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords形質評価 / QTL / ソルガム / 葉の老化 / 遺伝育種 / 遺伝育種学 / 環境ストレス
Outline of Final Research Achievements

Stay green is a physiological trait that is associated with nutrient redistribution during plant growth, but its precise role in adaptation to environmental stresses remain unclear. This study aims in genetically dissecting the missing link between stay green and its contribution to environments such as drought, in a large biomass crop sorghum. In this study, we employed Japanese landrace Takakibi, showing non-stay-green phenotype. A recombinant inbred population generated from the cross between Takakibi and stay-green BTx623 was used to characterize QTL associated with leaf greenness under natural growing condition. The linkage analysis allowed us to identify one QTL in chromosome 5. This gene appeared to be deleted in Takakibi, and we further investigated this gene as to whether it is responsible for the stay green trait.

Academic Significance and Societal Importance of the Research Achievements

秋になるとポプラの葉は落葉し、水田は一斉に黄金色となり実りの季節を迎える。「葉が枯れる」という現象は我々に最も身近な風景である一方、植物が自然環境に適応するための重要な生存戦略を具現している。植物にとって、葉がシンクとなり光合成を続けて栄養生長を続けるか、ソースとなって養分を提供して枯れていくかは、生存や繁栄を決定するトレードオフであり、この「葉の老化」制御を遺伝素因として明らかにすることは、バイオマスなど植物の利活用、あるいは資源保全などへ貢献する研究基盤となる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results)

  • [Int'l Joint Research] ジョモケニアッタ農工大学(ケニア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] RAD-seq-Based High-Density Linkage Map Construction and QTL Mapping of Biomass-Related Traits in Sorghum Using a Japanese Landrace Takakibi NOG2020

    • Author(s)
      Hiromi Kajiya-Kanegae1, Hideki Takanashi, Masaru Fujimoto, Motoyuki Ishimori, Norikazu Ohnishi, Fiona Wacera W., Everlyne A. Omollo, Masaaki Kobayashi, Kentaro Yano, Michiharu Nakano, Toshiaki Kozuka, Makoto Kusaba, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Wataru Sakamoto
    • Journal Title

      Plant and Cell Physiology

      Volume: 61

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photosynthetic Responses to High Temperature and Strong Light Suggest Potential Post-flowering Drought Tolerance of Sorghum Japanese Landrace Takakibi2019

    • Author(s)
      Ohnishi Norikazu、Wacera W. Fiona、Sakamoto Wataru
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 9 Pages: 2086-2099

    • DOI

      10.1093/pcp/pcz107

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Genetic dissection of aphid resistance in a sorghum cultivar2020

    • Author(s)
      Everlyne Omollo, Norikazu Ohnishi, Murage Hunja, Hiromi Kanegae, Ivan Galis, Wataru Sakamoto
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] QTL analysis of stay green in sorghum using a RIL population derived from Takakibi NOG2020

    • Author(s)
      荊 子桓、坂本 亘
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] たかきびRILのイオノームを用いたソルガム種子の元素含量に関するQTL解析2020

    • Author(s)
      ワセラ フィオナ ,藤原 徹,山崎 清志,高梨 秀樹,堤 伸浩,鐘ヶ江 弘美,坂本 亘
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Genetic Dissection of Apid Resistance in a Sorghum Cultivar2019

    • Author(s)
      Everlyne Omollo, Ivan Galis, 坂本 亘
    • Organizer
      第10回ソルガムワークショップ
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification of a stay-green gene in sorghum2019

    • Author(s)
      荊 子桓、坂本 亘
    • Organizer
      第10回ソルガムワークショップ
    • Related Report
      2019 Annual Research Report
  • [Presentation] 葉緑体膜機能維持に関わるVIPP1のGTPase活性の解析2019

    • Author(s)
      塩屋健一,大西 紀和,坂本 亘
    • Organizer
      第60回 日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Phenomics に適した QTL 解析手法の提案: ソルガ ム RIL 集団の葉形態を例に2018

    • Author(s)
      坂本 莉沙, 藤本 優, 高梨 秀樹, 鐘ケ江 弘美, 野下 浩司, 小林 正明, 矢野 健太郎, 小童谷 利恵, 大西 紀和, 堤 伸浩, 坂本 亘, 岩田 洋 佳
    • Organizer
      日本育種学会 第134回講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] ソルガムの在来種たかきびが示す高温・強光応答の出穂前後における変化の光合成活性測定による解析2018

    • Author(s)
      大西 紀和,坂本 亘
    • Organizer
      第9回 ソルガムワークショップ
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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