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Synergistic activation of ion channel by intracellular temperature and mechanical stimuli

Research Project

Project/Area Number 18K19418
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 48:Biomedical structure and function and related fields
Research InstitutionUniversity of Nagasaki (2020)
Gunma University (2018-2019)

Principal Investigator

Shibasaki Koji  長崎県立大学, 看護栄養学部, 教授 (20399554)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsTRPV2 / メカノセンサー / 温度 / 網膜剥離 / 機械刺激 / TRPV4 / 神経 / リハビリ / 発生 / TRPチャネル / 軸索伸長 / 発熱
Outline of Final Research Achievements

The passive stretching-dependent axonal outgrowth occurs in our body. It has
never been identified for a long time which molecules are the mechanosensors for it. We previously reported that TRPV2 was a mechanosensor channel which contributed axonal outgrowth in membrane stretch dependent manner. These results indicate that TRPV2 might be an important component for passive stretching under specific intracellular temperature, if TRPV2 can detect very weak mechanical stimulus at higher intracellular temperature. In this study, we examined whether TRPV2 can detect such very weak mechanical stimulus under specific intracellular temperature by a Ca2+-imaging method and a whole-cell patch clamp recording. We also examined whether the activation of TRPV2 by weak mechanical stimulus lead to the enhancement of axonal outgrowth. Finally, we identified that TRPV2 had a potential to detect very weak mechanical stimulus only under specific intracellular temperature.

Academic Significance and Societal Importance of the Research Achievements

本知見を応用して、病態原因解明の研究にも取り組んだ。TRPV2の類縁分子であり、TRPV2同様にメカノセンサー、温度センサーとして機能するTRPV4に着目し、網膜剥離の病態悪化機構を解析した。網膜剥離は強度の近視や糖尿病、ボクサーのように眼に強い刺激を受けることにより発症する。年間1万人に1人が発症すると考えられており、誰もが患者になる可能性がある失明を伴う重篤な疾患である。しかしながら、どのような分子メカニズムで病態悪化が進行するのかよく分かっていなかった。今回、研究実施者の研究からそのメカニズムの全貌が明らかとなり、新たな治療法を見出した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (32 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 5 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] ユタ大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ルーベン大学(ベルギー)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ルーベン大学(ベルギー)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Transient Receptor Potential Melastatin 3 Is Functionally Expressed in Oligodendrocyte Precursor Cells and Is Upregulated in Ischemic Demyelinated Lesions2021

    • Author(s)
      Ohashi Kana、Shibasaki Koji、Nakazawa Hayaki、Kunimasa Ryotaro、Nagayasu Kazuki、Shirakawa Hisashi、Kaneko Shuji
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 44 Issue: 2 Pages: 181-187

    • DOI

      10.1248/bpb.b20-00510

    • NAID

      130007979613

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2021-02-01
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Choroidal congestion mouse model: Could it serve as a pachychoroid model?2021

    • Author(s)
      Matsumoto H, Mukai R, Hoshino J, Ishizaki Y, Shibasaki K, Akiyama H.
    • Journal Title

      PLoS ONE

      Volume: 16(1) Issue: 1 Pages: 590-595

    • DOI

      10.1371/journal.pone.0246115

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transient receptor potential vanilloid 4 agonist GSK1016790A improves neurological outcomes after intracerebral hemorrhage in mice2020

    • Author(s)
      Asao Yasunori、Tobori Shota、Kakae Masashi、Nagayasu Kazuki、Shibasaki Koji、Shirakawa Hisashi、Kaneko Shuji
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 529 Issue: 3 Pages: 590-595

    • DOI

      10.1016/j.bbrc.2020.06.103

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRPV4 activation by thermal and mechanical stimuli in disease progression.2020

    • Author(s)
      Shibasaki K
    • Journal Title

      Lab. Invest.

      Volume: 100 Issue: 2 Pages: 218-223

    • DOI

      10.1038/s41374-019-0362-2

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] TRPC5 regulates axonal outgrowth in developing retinal ganglion cells.2020

    • Author(s)
      Oda M, Yamamoto H, Matsumoto H, Ishizaki Y, *Shibasaki K
    • Journal Title

      Lab. Invest.

      Volume: 100 Issue: 2 Pages: 297-310

    • DOI

      10.1038/s41374-019-0347-1

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments.2019

    • Author(s)
      Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, Sadakata T
    • Journal Title

      J Neurosci

      Volume: 39(32) Issue: 32 Pages: 6339-6353

    • DOI

      10.1523/jneurosci.2002-18.2019

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temperature elevation in epileptogenic foci exacerbates epileptic discharge through TRPV4 activation2019

    • Author(s)
      Shibasaki Koji、Yamada Katsuya、Miwa Hideki、Yanagawa Yuchio、Suzuki Michiyasu、Tominaga Makoto、Ishizaki Yasuki
    • Journal Title

      Laboratory Investigation

      Volume: 100 Issue: 2 Pages: 274-284

    • DOI

      10.1038/s41374-019-0335-5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] The dynamics of revascularization after white matter infarction monitored in Flt1-tdsRed and Flk1-GFP mice2019

    • Author(s)
      Shimauchi-Ohtaki Hiroya、Kurachi Masashi、Naruse Masae、Shibasaki Koji、Sugio Shouta、Matsumoto Ken、Ema Masatsugu、Yoshimoto Yuhei、Ishizaki Yasuki
    • Journal Title

      Neuroscience Letters

      Volume: 692 Pages: 70-76

    • DOI

      10.1016/j.neulet.2018.10.057

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Retinal detachment-induced Müller glial cell swelling activates TRPV4 ion channels and triggers photoreceptor death at body temperature.2018

    • Author(s)
      Matsumoto H, Sugio S, Krizaj D, Akiyama H, Ishizaki Y, Gailly P, Shibasaki K
    • Journal Title

      J Neurosci.

      Volume: 38 Issue: 41 Pages: 8745-8758

    • DOI

      10.1523/jneurosci.0897-18.2018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microglial activation induces generation of oligodendrocyte progenitor cells from the sub ventricular zone after focal demyelination in the corpus callosum.2018

    • Author(s)
      Naruse M, Shibasaki K, Shimauchi-Ohotaki H, Ishizaki Y
    • Journal Title

      Dev Neurosci

      Volume: 40 Issue: 1 Pages: 54-63

    • DOI

      10.1159/000486332

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] TRPV4 is functionally expressed in oligodendrocyte precursor cells and increases their proliferation2018

    • Author(s)
      Ohashi Kana、Deyashiki Ayane、Miyake Takahito、Nagayasu Kazuki、Shibasaki Koji、Shirakawa Hisashi、Kaneko Shuji
    • Journal Title

      Pflugers Archiv - European Journal of Physiology

      Volume: 470 Issue: 5 Pages: 705-716

    • DOI

      10.1007/s00424-018-2130-3

    • NAID

      120007006244

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of TRPV3 and TRPM8 ion channel proteins in induction of mammalian cold-inducible proteins2018

    • Author(s)
      Fujita Takanori、Liu Yu、Higashitsuji Hiroaki、Itoh Katsuhiko、Shibasaki Koji、Fujita Jun、Nishiyama Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 495 Issue: 1 Pages: 935-940

    • DOI

      10.1016/j.bbrc.2017.11.136

    • NAID

      120006460063

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] High temperature region heats up TRPV2-mechanosensor function and axonal outgrowth2021

    • Author(s)
      柴崎貢志
    • Organizer
      日本生理学会・日本解剖学会合同大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Hippocampal TRPV4 regulates neuronal excitability by brain temperature2020

    • Author(s)
      Shibasaki K
    • Organizer
      Forum of European Neuroscience
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Glial cells inhibit neuronal excitation through TRPV4 activation by body temperature.2020

    • Author(s)
      柴崎貢志
    • Organizer
      日本神経科学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Retinal detachment-induced Muller glial cell swelling activates TRPV4 ion channels and triggers photoreceptor death at body temperature.2019

    • Author(s)
      Shibasaki K, Matsumoto H, Sugio S, Krizaj D, Akiyama H, Ishizaki Y, Gailly P
    • Organizer
      GLIA2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Glial swelling evokes neuronal cell death through a temperature and mechano-sensor ion channel, TRPV4.2019

    • Author(s)
      柴崎貢志、杉尾翔太、Krizaj D、石崎泰樹、Gailly P.、松本英孝
    • Organizer
      Neuro2019
    • Related Report
      2019 Research-status Report
  • [Presentation] High temperature regions in growth cone enhance TRPV2-mechanosensor activity and axonal outgrowth2019

    • Author(s)
      織田麻衣、杉尾翔太、岡部弘基、岩田裕子、小野勝彦、石崎泰樹、柴崎貢志
    • Organizer
      Neuro2019
    • Related Report
      2019 Research-status Report
  • [Presentation] 脳内温度と神経活動の機能相関; TRPV4による調節2019

    • Author(s)
      柴崎貢志
    • Organizer
      Thermal Biology Workshop
    • Related Report
      2019 Research-status Report
  • [Presentation] TRPV4 ion channels and triggers photoreceptor death at body temperature.2019

    • Author(s)
      Shibasaki K, Matsumoto H, Sugio S, Krizaj D, Akiyama H, Ishizaki Y, Gailly P
    • Organizer
      FAOPS
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] TRPV4 activation triggers ATP release from Müller glial cells.2019

    • Author(s)
      Sugio S, Oda M, Ishizaki Y, , Shibasaki K
    • Organizer
      FAOPS
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Retinal detachment-induced Müller glial cell swelling activates TRPV4 ion channels and triggers photoreceptor death at body temperature.2018

    • Author(s)
      Shibasaki K, Matsumoto H, Sugio S, Krizaj D, Akiyama H, Ishizaki Y, Gailly P
    • Organizer
      ARVO annual meeting (Hawaii)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] メカノセンサーTRPV2が軸索伸長を促す分子基盤2018

    • Author(s)
      柴崎貢志、杉尾翔太、岡部弘基、岩田裕子、小野勝彦、石崎泰樹
    • Organizer
      第41回日本神経科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] グリア細胞においてはTRPV4活性化によりATP放出が惹起する2018

    • Author(s)
      杉尾翔太、織田麻衣、石崎泰樹、柴崎貢志
    • Organizer
      第41回日本神経科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Mechanosensor function of TRPV2 is sensitized by high temperature region in growth cones, and promotes axonal outgrowth during development.2018

    • Author(s)
      織田麻衣、杉尾翔太、岡部弘基、岩田裕子、小野勝彦、石崎泰樹、柴崎貢志
    • Organizer
      第61回日本神経化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 成長円錐内の高温部位がTRPV2活性化と軸索伸長を促進する2018

    • Author(s)
      柴崎貢志
    • Organizer
      第91回日本生化学学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks]

    • URL

      https://sun.ac.jp/researchinfo/kshibasaki/

    • Related Report
      2020 Annual Research Report
  • [Remarks]

    • URL

      https://www.researchgate.net/profile/Koji_Shibasaki

    • Related Report
      2019 Research-status Report
  • [Remarks] 柴崎貢志の研究内容

    • URL

      http://www.nips.ac.jp/cs/sibaHP/shibasaki.html

    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2022-01-27  

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