| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The purpose of this study is to develop a method to prepare supported lipid bilayers with simultaneous control of membrane protein orientation and lipid membrane asymmetry, and to observe the dynamics of membrane proteins whose function is related to lipid membrane asymmetry by high-speed AFM. By FY 2019, planar membranes were prepared on AFM substrates by the droplet contact method. Using a micromanipulator and a microinjector on the sample stage of a tip-scanning high-speed AFM, we were able to prepare a uniform lipid film on the AFM substrate. However, there was a problem that the optical system of the tip-scanning high-speed AFM was unstable. Therefore, in FY2021, we replaced the tip-scanning high-speed AFM with a more stable sample-scanning high-speed AFM and adjusted it to a level where it could operate stably.
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