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Analysis of cellular hierarchy by transcribed DNA barcodes

Research Project

Project/Area Number 18K19471
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 50:Oncology and related fields
Research InstitutionChiba University (2019)
The University of Tokyo (2018)

Principal Investigator

Seki Motoaki  千葉大学, 医学部附属病院, 特任助教 (70714278)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsDNAバーコード / 癌オルガノイド / 細胞系譜 / 癌幹細胞
Outline of Final Research Achievements

In this study, we developed a technology in which each cell in a heterogeneous population is tagged with arbitrary DNA barcodes to link gene expression profiles and chromatin accessibility to the clone information. We established gastric mucosal epithelial cells with the DNA barcodes to be infected with Epstein-Barr virus. We are analyzing the clones enriched post-infection and their transcriptomic and epigenomic status.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した一細胞遺伝子発現解析およびエピゲノム解析において判読可能なDNAバーコードライブラリーおよび不均一な細胞集団における細胞系譜情報と細胞プロファイルを紐付ける技術は、発癌や組織の発生、分化過程など、多様な細胞から構成される細胞集団の階層性やその振る舞いを理解する上で有用である。従って、前述のような生命現象の理解に加え、抗癌剤耐性の獲得機序やiPS細胞からの分化誘導過程などの解析から、治療薬開発あるいは再生医療への応用へも期待できる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice2020

    • Author(s)
      Nishizono Hirofumi、Darwish Mohamed、Uosaki Hideki、Masuyama Nanami、Seki Motoaki、Abe Hiroyuki、Yachie Nozomu、Yasuda Ryohei
    • Journal Title

      Journal of Visualized Experiments

      Volume: - Issue: 158 Pages: 1-1

    • DOI

      10.3791/60808

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A genome-scale CRISPR/Cas9 knockout screening reveals SH3D21 as a sensitizer for gemcitabine2019

    • Author(s)
      Masoudi Mohammad、Seki Motoaki、Yazdanparast Razieh、Yachie Nozomu、Aburatani Hiroyuki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 19188-19188

    • DOI

      10.1038/s41598-019-55893-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Engineered CRISPR-Cas9 nuclease with expanded targeting space2018

    • Author(s)
      Nishimasu Hiroshi、Shi Xi、Ishiguro Soh、Gao Linyi、Hirano Seiichi、Okazaki Sae、Noda Taichi、Abudayyeh Omar O.、Gootenberg Jonathan S.、Mori Hideto、Oura Seiya、Holmes Benjamin、Tanaka Mamoru、Seki Motoaki、Hirano Hisato、Aburatani Hiroyuki、Ishitani Ryuichiro、Ikawa Masahito、Yachie Nozomu、Zhang Feng、Nureki Osamu
    • Journal Title

      Science

      Volume: 361 Issue: 6408 Pages: 1259-1262

    • DOI

      10.1126/science.aas9129

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 診療施設併設型バイオバンクとアカデミア解析・教育センターにおけるバイオバンク連携.2019

    • Author(s)
      3.関 元昭、加野将之、飯田慶子、小林嵩平、鬼澤 歩、小松亜樹、村上健太郎、糸賀 栄、衣鳩 恵、西村 基、吉富秀幸、野島広之、大岡美彦、大和田千佳子、坂本信一、丸山陽佳、中島崇裕、島井健一郎、荒屋敷亮子、田村 裕、鈴木隆弘、花岡英紀、松下一之、太田 聡、大塚将之、市川智彦、松原久裕、金田篤志、滝口裕一.
    • Organizer
      第5回クリニカルバイオバンク学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] In-human assembly: efficient DNA assembly in cultured mammalian cells2019

    • Author(s)
      Motoaki Seki, Soh Ishiguro, Kentaro Kakoi & Nozomu Yachie
    • Organizer
      LSBM Symposium 2019
    • Related Report
      2019 Annual Research Report

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Published: 2018-07-25   Modified: 2021-02-19  

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