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Elucidation of osteoblast fate determination by single cell analysis on mRNA-ncRNA

Research Project

Project/Area Number 18K19647
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 57:Oral science and related fields
Research InstitutionHiroshima University

Principal Investigator

Yoshiko Yuji  広島大学, 医系科学研究科(歯), 教授 (20263709)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨芽細胞 / 多様性 / 運命決定 / シングルセルRNA-Seq / 非翻訳RNA / 最終分化 / マウス / Cd34 / ncRNA / 骨細胞 / lining cell / シングルセル RNA-Seq / シングルセル 解析 / 細胞運命決定
Outline of Final Research Achievements

The aim of this study is to elucidate epigenetic regulation of the diversity and fate determination of osteoblasts. Single-cell RNA-Seq analysis of mouse osteoblasts isolated from reporter mice expressing Venus in osteoblasts was conducted to determine non-coding RNA (ncRNA) expression profiling. Clustering analysis of these RNAs identified a unique population of osteoblasts that was in good agreement with a cell cluster exhibiting unusual traits in our previous report. Three ncRNAs specifically expressed in this cluster were analyzed in silico, suggesting that osteoblasts in this cluster may maintain multipetency.

Academic Significance and Societal Importance of the Research Achievements

骨形成後の骨芽細胞は大部分死滅するが、一部は骨細胞へと最終分化し、長寿命を獲得して骨代謝を制御する。また、少数はlining cellとして骨表面に留まり、必要に応じて再び骨芽細胞となると考えられている。このように骨芽細胞はその運命決定において多様性を持つと考えれれるが、その制御機構は不明である。骨芽細胞の解析は、これまではバルクが主流で、個々の骨芽細胞が着目されることはほとんどなかった。シングルセルレベルでの解析はこの問題解決のブレークスルーとなる可能性があり、多様性の分子基盤が明らかになれば、学術的意義のみならず、骨の再生治療や様々な骨疾患の治療に新たな見識を提供することになる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2021 2019 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Institut de Cancerologie de l’Ouest(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Single‐cell RNA‐sequencing reveals the breadth of osteoblast heterogeneity2021

    • Author(s)
      Yoshioka H, Okita S, Nakano M, Minamizaki T, Nubukiyo A, Sotomaru Y, Bonnelye E, Kozai K, Tanimoto K, Aubin JE, Yoshiko Y
    • Journal Title

      JBMR Plus

      Volume: - Issue: 6

    • DOI

      10.1002/jbm4.10496

    • NAID

      120007183697

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シングルセル解析から見た骨芽細胞の多様性と脂肪細胞分化転換能2019

    • Author(s)
      中野将志,吉岡広陽,南崎朋子,香西克之,吉子裕二
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] A new paradigm for bone turnover and its potential clinical implications2019

    • Author(s)
      Yuji Yoshiko
    • Organizer
      International Dental Scientific Conference and Expo, Dies Forum 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-07-25   Modified: 2022-01-27  

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