• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new technology for taste cell omics: exploring molecular logics of high calorie sensing and taste signal transduction

Research Project

Project/Area Number 18K19653
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 57:Oral science and related fields
Research InstitutionKyushu University

Principal Investigator

Ninomiya Yuzo  九州大学, 五感応用デバイス研究開発センター, 特任教授 (50076048)

Co-Investigator(Kenkyū-buntansha) 吉田 竜介  岡山大学, 医歯薬学総合研究科, 教授 (60380705)
安松 啓子  東京歯科大学, 歯学部, 准教授 (50380704)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords脂質味 / 味受容伝達関連分子 / RNA-seq法 / カロリー受容経路 / 高嗜好形成 / RNA-Seq法
Outline of Final Research Achievements

The present study predicted fat taste- and calorie sensing-related molecules based on comprehensive gene analysis of mouse single taste cells, and made functional analyses on their expression in taste cells and potential relations to taste nerve and behavioral responses to various taste stimuli. The results showed that, in addition to known taste information pathways for each of 5 basic tastes (sweet, sour, salt, bitter and umami), there exist two different taste neural pathways for fat perception: one is a fat taste-specific pathway derived from cells expressing fat receptors such as GPR120 etc, and the other is a calorie sensing pathway derived from cells co-expressing multiple transporters and receptors for lipids and sugars, and metabolic sensor channels (KATP). Thus, the new taste cell omics technology, which is a fusion of taste cell comprehensive gene analysis and taste response analysis, has led to the new findings and its effectiveness was suggested.

Academic Significance and Societal Importance of the Research Achievements

本研究は、近年の肥満者増加の背景にある脂質の味と高嗜好性形成の原理を解明するため、味細胞における遺伝子・分子発現と味細胞・神経・行動応答を解析した。その結果、味細胞から脳に伝えられる脂質味情報は、味の弁別に必要な脂質特異的な経路とは別に、食欲や代謝センサーによる調節を受け、脂質・糖などのカロリー受容高嗜好性に関わる味経路を介しても伝えられることを見出した。この新たな脂質・糖カロリー受容高嗜好味情報経路の発見は、脂質の高嗜好性形成原理の一端を明らかにし、食の健全化への今後の新たな研究展開への起点となるものであり、学際的意義のみならず社会的意義も高いものと思われる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (46 results)

All 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (35 results) (of which Int'l Joint Research: 15 results,  Invited: 14 results) Book (1 results) Remarks (4 results)

  • [Int'l Joint Research] モネル化学感覚研究所(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] モネル化学感覚研究所(米国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues2020

    • Author(s)
      Ohki Junko、Sakashita Akihiko、Aihara Eitaro、Inaba Akihiko、Uchiyama Hironobu、Matsumoto Masahito、Ninomiya Yuzo、Yamane Takumi、Oishi Yuichi、Iwatsuki Ken
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 84(5) Issue: 5 Pages: 936-942

    • DOI

      10.1080/09168451.2020.1713043

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of Renin-Angiotensin System Components in the Taste Organ of Mice2019

    • Author(s)
      Shigemura Noriatsu、Takai Shingo、Hirose Fumie、Yoshida Ryusuke、Sanematsu Keisuke、Ninomiya Yuzo
    • Journal Title

      Nutrients

      Volume: 11 Issue: 9 Pages: 2251-2251

    • DOI

      10.3390/nu11092251

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of insulin signaling on mouse taste cell proliferation2019

    • Author(s)
      Takai Shingo、Watanabe Yu、Sanematsu Keisuke、Yoshida Ryusuke、Margolskee Robert F.、Jiang Peihua、Atsuta Ikiru、Koyano Kiyoshi、Ninomiya Yuzo、Shigemura Noriatsu
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 11 Pages: 1-16

    • DOI

      10.1371/journal.pone.0225190

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fatty acid taste quality information via GPR120 in the anterior tongue of mice2018

    • Author(s)
      Yasumatsu Keiko、Iwata Shusuke、Inoue Mayuko、Ninomiya Yuzo
    • Journal Title

      Acta Physiologica

      Volume: 226 Issue: 1

    • DOI

      10.1111/apha.13215

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 甘味受容体感受性における低pHによる抑制効果の分子メカニズム2020

    • Author(s)
      實松 敬介,二ノ宮 裕三,重村 憲徳
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 低濃度塩添加によるヒト甘味認知閾値の低下2020

    • Author(s)
      吉田 竜介,實松 敬介,高井 信吾,岩田 周介,重村 憲徳
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Signal detection and transmission pathways for sugars and fatty acids in the peripheral taste systems.2019

    • Author(s)
      Ninomiya Yuzo
    • Organizer
      39th Blankrnese Conferences.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 味細胞オルガノイドを用いた味細胞分化/増殖に影響を及ぼす因子の探索2019

    • Author(s)
      髙井 信吾,Robert F. Margolskee,Peihua Jiang,二ノ宮裕三,重村 憲徳
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 脂肪酸受容体の舌における部位的・時間的機能分担2019

    • Author(s)
      安松啓子,二ノ宮裕三
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] アドレノメデュリンによる T1Rs 非依存性経路を介するマウス鼓索神経糖 応答への影響2019

    • Author(s)
      岩田 周介,井上真由子,吉田 竜介,二ノ宮裕三
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マウス味細胞におけるmechanistic target of rapamycin (mTOR)の働き2019

    • Author(s)
      高井信吾,Margolskee Robert. F,Jiang Peihua ,二ノ宮裕三,重村 憲徳
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] T1R3-KOマウスの鼓索神経単一神経記録および行動応答測定によるグルコースの味覚情報解析2019

    • Author(s)
      安松啓子,岩田周介,二ノ宮裕三
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アドレノメデュリンによるT1Rs非依存性経路を介したマウス鼓索神経応答への影響2019

    • Author(s)
      岩田周介,井上真由子,吉田竜介,二ノ宮裕三
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Taste Session:Overview2019

    • Author(s)
      Ninomiya Yuzo
    • Organizer
      The 48th Naito Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Contribution of SGLTs to sugar sensing as T1R-independent mechanisms in mouse gustatory tissues.2019

    • Author(s)
      Yasumatsu Keiko, Ohkuri Tadahiro, Iwata Shusuke, Margolskee Robert F, Ninomiya Y.uzo
    • Organizer
      The 18th ISMNTOP
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Signaling cascade for the sweet suppressive effect of leptin.2019

    • Author(s)
      Yoshida Ryusuke, Margolskee Robert F, Ninomiya Yuzo.
    • Organizer
      The 18th ISMNTOP
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The effect of Adrenomedullin on chorda tympani nerve responses to sugars via T1rs-independent pathway in mice.2019

    • Author(s)
      Iwata Shusuke, Inoue Mayuko, Yoshida Ryusuke, Ninomiya Yuzo.
    • Organizer
      The 18th ISMNTOP
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Insulin - mTOR signaling regulate taste bud organoid growth.2019

    • Author(s)
      Takai Shingo, Margolskee Robert F, Jiang Peihua, Ninomiya Yuzo, Shigemura Noriatsu.
    • Organizer
      The 18th ISMNTOP
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 未知の味・カロリーシグナル伝達経路の探索と食調節2019

    • Author(s)
      二ノ宮裕三
    • Organizer
      千里ライフサイエンスセミナーN4(感覚のサイエンス~豊かな社会の実現に向けて~)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Novel taste sensory pathways for sugars and fatty acids in the mouse periphery.2019

    • Author(s)
      Ninomiya Y, Yasumatsu K, Iwata S, Yoshida R.
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fatty acid-responding neurons in mouse glossopharyngeal nerve.2019

    • Author(s)
      Yasumatsu K, Iwata S, Inoue M, Ninomiya Y.
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Involvement of phosphoinositide 3-kinase in leptin signaling in sweet sensitive taste cells.2019

    • Author(s)
      Yoshida R, Margolskee. RF, Ninomiya Y.
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effects of insulin signaling on mouse taste bud organoid.2019

    • Author(s)
      Takai S, Jiang P, Margolskee RF, Ninomiya Y, Shigemura N.
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Adrenomedullin enhances chorda tympani nerve responses to sugars in mice.2019

    • Author(s)
      Iwata S, Inoue M, Yasumatsu K, Yoshida R, Ninomiya Y.
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Altered expression of taste signaling elements in jejunal tissue of obese patients.2019

    • Author(s)
      Yasuo T, Jiang P, Wood C, Chu Z, Benotti P, Still C, Rolston DD, Margolskee RF, Ninomiya Y.
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Roles of sugar transporters and calorie sensors in taste cells.2018

    • Author(s)
      Ninomiya Y, Yasumatsu K, Yoshida R, Iwata S, Shigemura N.
    • Organizer
      ECRO2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス舌咽神経における脂肪酸に対する単一神経応答の解析2018

    • Author(s)
      安松啓子,岩田周介,二ノ宮裕三
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] アドレノメデュリンによるマウス鼓索神経応答への影響2018

    • Author(s)
      岩田周介,安松啓子,吉田竜介,二ノ宮裕三
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 末梢における甘味の受容と調節メカニズム2018

    • Author(s)
      吉田竜介
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] グルカゴン様ペプチド-1 受容体遺伝子多型と甘味認知閾値との関連について2018

    • Author(s)
      實松敬介 井上真由子,髙井信吾,二ノ宮裕三
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] インスリンシグナルは味細胞の分化・増殖に関与する2018

    • Author(s)
      髙井信吾,Robert F. Margolskee,Peihua Jiang,二ノ宮裕三, 重村憲徳
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 味覚障害誘発薬剤がマウス味蕾オルガノイドへ与える影響2018

    • Author(s)
      川端由子,高井信吾,吉田竜介,實松敬介,重村憲徳
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 味細胞におけるレプチンシグナリング2018

    • Author(s)
      吉田竜介
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] マウス舌咽神経単一神経記録による脂肪酸受容機構の解析2018

    • Author(s)
      安松啓子,岩田周介,井上真由子,二ノ宮裕三
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 低pH条件下における甘味受容体感受性の解析2018

    • Author(s)
      實松敬介,尾崎礼奈,中林開人,二ノ宮裕三,重村憲徳
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] マウス味細胞の分化/増殖におけるインスリンの効果2018

    • Author(s)
      高井信吾,Margolskee Robert. F,Jiang Peihua ,二ノ宮裕三,重村 憲徳
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] アドレノメデュリンのマウス鼓索神経応答への影響2018

    • Author(s)
      岩田周介,安松啓子,井上真由子,吉田竜介,二ノ宮裕三
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Leptin signaling pathway linking from activation of leptin receptor to KATP channel activation in sweet sensitive taste cells in mice.2018

    • Author(s)
      Yoshida R, Margolskee RF ,Ninomiya Y.
    • Organizer
      17th ISMNTOP
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The potential effects of insulin signaling on mouse taste bud organoid.2018

    • Author(s)
      Takai S, Margolskee RF , Jiang P, Ninomiya Y, Shigemura N.
    • Organizer
      17th ISMNTOP
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 日本歯科評論 No.915 79/1 2019年1月号2019

    • Author(s)
      二ノ宮裕三
    • Total Pages
      5
    • Publisher
      ヒョーロン・パブリッシャーズ
    • Related Report
      2018 Research-status Report
  • [Remarks] 九州大学 五感応用デバイス研究開発センター

    • URL

      http://www.rdctos.kyushu-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 岡山大学大学院医歯薬学総合研究科口腔生理学分野

    • URL

      http://www.cc.okayama-u.ac.jp/~oralphys/OralPhysiology.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 九州大学 五感応用デバイス研究開発センター

    • URL

      http://www.rdctos.kyushu-u.ac.jp/

    • Related Report
      2018 Research-status Report
  • [Remarks] 九州大学大学院 歯学研究院 口腔機能解析学分野

    • URL

      http://www.dent.kyushu-u.ac.jp/sosiki/a06/

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-07-25   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi