| Project/Area Number |
18K19740
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 筋線維 / 遅筋 / 速筋 / モノクローナル抗体 / 骨格筋 / 発生 |
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| Outline of Final Research Achievements |
The antigen recognized by the 3A11-3 antibody is probably a 150-160 kDa membrane protein localized in the cell membrane of neonatal muscle tissue. 3A11-3 antibody is IgM and does not bind to the antigen as strongly as IgG. Therefore, it is thought that the antigen could not be efficiently recovered and identified by LC-MS/MS. As mentioned above, IgM is thought to have a weak binding force, so it is possible that the antigen is dissociated by washing the resin during pull-down. In the future, we will improve the efficiency of protein extraction from the electrophoresis bands detected by silver staining and continue to identify the proteins by LC-MS/MS.
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| Academic Significance and Societal Importance of the Research Achievements |
これまでの筋線維タイプの決定している因子は，筋原繊維を構成するミオシンやミトコンドリアタンパクなど，その殆ど全てが細胞内タンパクである。そのため，細胞表面タンパク質が，筋線維タイプにより異なる成果は，細胞外のシグナルを介して筋線維タイプが決定される可能性を示しており，学術的には非常に新規性の高い成果である。さらに，本抗原は，筋線維タイプが決定されると，その発現が低下することから，筋線維タイプの維持ではなく，その決定に関与している可能性が高い。今回は抗原の同定には至らなかったが，その同定により様々な病態で変化する筋線維タイプの変動機能回目につながる可能性が期待される。
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