Estimation of microbial nitrogen cycle based on triple oxygen isotopes of nitrate

• Project/Area Number: 18K19850
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 63:Environmental analyses and evaluation and related fields
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Hattori Shohei 東京工業大学, 物質理工学院, 研究員 (70700152)
• Co-Investigator(Kenkyū-buntansha): 竹内 望 千葉大学, 大学院理学研究院, 教授 (30353452)
• Project Period (FY): 2018-06-29 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 窒素循環 / 三酸素同位体組成 / 氷河 / 暗色化 / 硝酸 / 硝化 / 脱窒 / アルベド / クリオコナイト

Outline of Final Research Achievements

This study aimed to detect nitrogen cycling in glacier ecosystems using the triple oxygen isotopic composition of nitrate. The triple oxygen isotopic composition of nitrate is high (+26‰) for the atmospheric deposition but nitrate derived from microbial nitrification possesses 0‰. Therefore, it is possible to distinguish between air-derived nitric acid and nitrification-derived nitric acid. Based on this analysis applied to the Asian glaciers, microbial nitrification reactions occur inside of these glaciers.

Academic Significance and Societal Importance of the Research Achievements

氷河の暗色化は2000年以降になって現れ始めた新しい現象であり、近年その重要性の認知とともに研究が発展している。このため、その要因となる氷河微生物及び氷河生態系の維持に必要な制限要因の理解は今後の氷河暗色化の予測には必須である。その一つの有力な候補が窒素であり、本研究は、これまでほとんど研究のない氷河生態系の窒素循環を、独自の安定同位体分析手法によって明らかとした。

Research Products

• [Int'l Joint Research] Chinese Academy of Sciences/Guangzhou Institute of Geochemistry(中国)
• [Int'l Joint Research] USCD(米国)
• [Int'l Joint Research] Chinese Academy of Sciences(中国)
• [Journal Article] A Complete Isotope (δ 15 N, δ 18 O, Δ 17 O) Investigation of Atmospherically Deposited Nitrate in Glacial‐Hydrologic Systems Across the Third Pole Region (2020)
  • Author(s): Lin Mang、Hattori Shohei、Wang Kun、Kang Shichang、Thiemens Mark H.、Yoshida Naohiro
  • Journal Title: Journal of Geophysical Research: Atmospheres Volume: 125 Issue: 19
  • DOI: 10.1029/2019jd031878
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Isotopic constraints on the formation pathways and sources of atmospheric nitrate in the Mt. Everest region (2020)
  • Author(s): Wang Kun、Hattori Shohei、Kang Shichang、Lin Mang、Yoshida Naohiro
  • Journal Title: Environmental Pollution Volume: 267 Pages: 115274-115274
  • DOI: 10.1016/j.envpol.2020.115274
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isotopic evidence for seasonality of microbial internal nitrogen cycles in a temperate forested catchment with heavy snowfall (2019)
  • Author(s): Hattori Shohei、Nunez Palma Yoshio、Itoh Yuko、Kawasaki Moeko、Fujihara Yoichi、Takase Keiji、Yoshida Naohiro
  • Journal Title: Science of The Total Environment Volume: 690 Pages: 290-299
  • DOI: 10.1016/j.scitotenv.2019.06.507
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 硝酸の三酸素同位体組成による氷河内部における微生物窒素循環の検出 (2020)
  • Author(s): 服部祥平、竹内望、吉田尚弘
  • Organizer: 日本地球化学会第67回年会
• [Presentation] Isotopic constrains on sources and formation pathways of atmospheric nitrate in Mt. Everest (2019)
  • Author(s): Kun Wang、Shohei Hattori、Shichang Kang、Mang Lin、Naohiro Yoshida
  • Organizer: 日本地球惑星科学連合2019年大会
• [Presentation] 東南極における大気硫酸の硫黄安定同位体組成の季節変動と大気硫黄循環への示唆 (2019)
  • Author(s): 石野 咲子、服部 祥平、Joel Savarino、Michel Legrand、Susanne Preunkert、Bruno Jourdain、Francis Albarede、Emmanuelle Albalat、吉田 尚弘
  • Organizer: 日本地球惑星科学連合2019年大会
• [Presentation] 高緯度北極スバールバルの氷河後退域における土壌硝化能の遷移 (2019)
  • Author(s): 林 健太郎、田邊 優貴子、小野 圭介、浅野 眞希、服部 祥平、内田 雅己、早津 雅仁
  • Organizer: 日本地球惑星科学連合2019年大会
• [Presentation] アルタイ山脈ベルーハ氷河コアから復元する過去100 年の硝酸同位体組成変動 (2019)
  • Author(s): 服部 祥平, Mang Lin, 竹内 望, 藤田 耕史, Caillon Nicolas, Akers Pete, Aizen Vladimir, Nikitin Stanislav, 吉田 尚弘, Savarino Joel
  • Organizer: 2019年度地球化学会年会
• [Presentation] アルタイ山脈ベルーハ氷河コアから復元する過去100 年の硝酸同位体組成変動 (2019)
  • Author(s): 服部祥平, Lin Mang, 竹内望, 藤田耕史, Nicolas Caillon, Pete Akers, Vladimir Aizen, Stanislav Nikitin, 吉田尚弘, Joel Savarino
  • Organizer: 雪氷研究大会（2019・山形）
• [Presentation] 山形県月山の樹林帯の融雪期の積雪表面に含まれる硝酸の起源 (2019)
  • Author(s): 渡邉茜, 服部祥平, 吉田尚弘, 竹内望
  • Organizer: 雪氷研究大会（2019・山形）
• [Presentation] Do oxygen isotopic anomalies in atmospheric nitrates vary along nitrate formation altitudes? (2018)
  • Author(s): Mang Lin, Shohei Hattori, Nozomi Suzuki, Xiufeng Yin, Qianggong Zhang, Shichang Kang, Mark H Thiemens, Naohiro Yoshida
  • Organizer: AGU Fall Meeting
  • Int'l Joint Research
• [Presentation] Determination of δ18O, Δ17O, and δ15N in atmospheric nitrates: First steps towards a deeper understanding of the nitrogen cycle over the Tibetan Plateau. (2018)
  • Author(s): Mang Lin, Shohei Hattori, Nozomi Suzuki, Shichang Kang, Naohiro Yoshida
  • Organizer: 2018 joint 14th iCACGP QS/15th IGAC SC, in Takamatsu, Kagawa, Japan, 25th to 29th of September 2018
  • Int'l Joint Research