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Challenges for cultivation and isolation of anaerobic fastidious microorganism using conductive microbial growth medium and its application to environmental engineering

Research Project

Project/Area Number 18K19873
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 64:Environmental conservation measure and related fields
Research InstitutionNagaoka University of Technology

Principal Investigator

HATAMOTO MASASHI  長岡技術科学大学, 工学研究科, 准教授 (20524185)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords廃水処理 / メタン / 微生物 / 嫌気共生細菌 / 導電性物質 / アーキア / 担体 / 嫌気性微生物 / 脱窒 / 電子伝達 / 嫌気共生
Outline of Final Research Achievements

Effective anaerobic microbial carrier, which was mixing the conductive materials into polyvinyl alcohol (PVA), was developed in this study. Using the newly developed microbial carrier the ratio of methanogens and anaerobic syntrophic microorganisms were largely increased compared to conventional PVA microbial carriers. This showed novel microbial carrier holds key microorganism of anaerobic digestion effectively. It was also possible to culture only the microorganisms retained in this carrier as a source of inoculation. For enrichment of denitrifying anaerobic methane oxidation microorganisms, the way of methane supplying is key parameters and direct supply of methane from atmosphere is more appropriate than membrane permeation.

Academic Significance and Societal Importance of the Research Achievements

微生物増殖担体に導電性物質を混合するだけという非常に簡単な方法でメタン発酵の鍵となるメタン生成古細菌や嫌気共生細菌を効率的に担体に保持できるため、メタン発酵法の安定化に貢献できる技術である。また嫌気性微生物の新しい培養方法としても活用できる成果であり、学術的意義も非常に高い。また嫌気性メタン酸化脱窒微生物の培養では基質であるメタンの供給方法が鍵であることがわかり、今後の研究開発の方向性の一つとなりうる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2019 Other

All Int'l Joint Research (1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] BIOTECH/KMUTT(タイ)

    • Related Report
      2019 Annual Research Report
  • [Presentation] Enhancing an anaerobic syntrophic propionate degradation using modified PVA gel beads2019

    • Author(s)
      Sitthakarn SITTHI, Masashi HATAMOTO, Takahiro WATARI, Takashi YAMAGUCHI
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microbial Promoting of DIET via modified PVA beads in syntrophic propionate degradation2019

    • Author(s)
      Sitthakarn SITTHI, Masashi HATAMOTO, Takahiro WATARI, Takashi YAMAGUCHI
    • Organizer
      12th International Conference on Challenges in Environmental Science & Engineering
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 導電性材料を介したDIETによるメタン生成の保進2019

    • Author(s)
      Nur Aina Afiqah Binti Abdul Rahman, Sitthakarn Sitthi, 幡本将史,渡利高大,山口隆司
    • Organizer
      第37回 土木学会関東支部新潟会研究調査発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 天然鉱物「十和田石」を用いたメタン生成に関与する微生物群の解析2019

    • Author(s)
      黒田恭平、延優、成廣隆、幡本将史、山口隆司、山内正仁、山田真義
    • Organizer
      第53回 日本水環境学会年会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 微生物担体及び廃液処理方法2019

    • Inventor(s)
      幡本 将史、シッタカーン シッティ、山口 隆司、渡利 高大
    • Industrial Property Rights Holder
      幡本 将史、シッタカーン シッティ、山口 隆司、渡利 高大
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-199827
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2018-07-25   Modified: 2021-02-19  

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