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Intelligent Artificial Cells that Respond to Histamine

Research Project

Project/Area Number 18K19944
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

Yokobayashi Yohei  沖縄科学技術大学院大学, 核酸化学・工学ユニット, 教授 (70769752)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords人工細胞 / リボスイッチ / 合成生物学 / RNA工学 / バイオセンサー / アプタマー / リポソーム
Outline of Final Research Achievements

In this project, we constructed a genetic switch called riboswitch that detect histamine (involved in allergy, etc.) and respond by synthesizing arbitrary protein. We then inserted the riboswitch in artificial membrane compartments to build artificial cells. These artificial cells can respond to externally added histamine by synthesizing fluorescent proteins or by making nanopores on the membrane to release chemical compounds inside the cells.

Academic Significance and Societal Importance of the Research Achievements

細胞の機能を、細胞を使わずに再現することを目指す、「人工細胞」の研究が進んできている。このような「人工細胞」は、我々が細胞の機能を理解するために役立つだけでなく、将来医療や環境分野での応用も期待される。本研究では、このような「人工細胞」に、化学物質に応答して、別の化合物を放出したり、自己破壊する機能を持たせた。このような研究の積み重ねにより「人工細胞」がより高度で複雑な機能を装備し、本物の細胞に近づき、さらには超えていくことを目指す。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (14 results)

All 2020 2019 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] セルフリーシステムにおける人工リボスイッチの構築2020

    • Author(s)
      横林 洋平
    • Journal Title

      生物工学

      Volume: 98 Pages: 655-658

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch2019

    • Author(s)
      Dwidar Mohammed、Seike Yusuke、Kobori Shungo、Whitaker Charles、Matsuura Tomoaki、Yokobayashi Yohei
    • Journal Title

      Journal of the American Chemical Society

      Volume: 141 Issue: 28 Pages: 11103-11114

    • DOI

      10.1021/jacs.9b03300

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Self-powered RNA nanomachine driven by metastable structure2019

    • Author(s)
      Kobori Shungo、Nomura Yoko、Yokobayashi Yohei
    • Journal Title

      Nucleic Acids Research

      Volume: 47 Issue: 11 Pages: 6007-6014

    • DOI

      10.1093/nar/gkz364

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles2019

    • Author(s)
      Nomura Yoko、Yokobayashi Yohei
    • Journal Title

      Nucleic Acids Research

      Volume: 47 Issue: 17 Pages: 8950-8960

    • DOI

      10.1093/nar/gkz729

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a histamine aptasensor for food safety monitoring2019

    • Author(s)
      Dwidar Mohammed、Yokobayashi Yohei
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 16659-16659

    • DOI

      10.1038/s41598-019-52876-1

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] セルフリーシステムにおける人工リボスイッチの構築2020

    • Author(s)
      横林 洋平
    • Organizer
      生物工学Webシンポジウム2020
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Designing RNA Devices in Test Tubes and Cells2019

    • Author(s)
      Yokobayashi Y
    • Organizer
      46th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 人工細胞でも使える(?)RNAデバイスの作成2019

    • Author(s)
      横林 洋平
    • Organizer
      「細胞を創る」研究会12.0
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Expanding Chemical Interface of Artificial Cells by Synthetic Riboswitch2019

    • Author(s)
      Yokobayashi Y, Dwidar M, Seike Y, Kobori S, Whitaker C, Matsuura T
    • Organizer
      Cell Free Systems Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] High-Throughput Screening of Cell-Free Riboswitches by Droplet Sorting2019

    • Author(s)
      Tabuchi T, Yokobayashi Y.
    • Organizer
      Cell Free Systems Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Systematic Minimization of RNA Ligase Ribozyme Through Large-Scale Design-Synthesis-Sequence Cycles2019

    • Author(s)
      Nomura Y, Yokobayashi Y.
    • Organizer
      46th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Remarks] ヒスタミンに応答する人工細胞を初めて合成

    • URL

      https://www.oist.jp/ja/news-center/press-releases/34046

    • Related Report
      2019 Research-status Report
  • [Remarks] Synthesizing Chemical-sensing Cells from Scratch

    • URL

      https://www.oist.jp/news-center/press-releases/synthesizing-chemical-sensing-cells-scratch

    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] AN RNA APTAMER THAT SPECIFICALLY BINDS HISTAMINE2020

    • Inventor(s)
      Yokobayashi Y, Dwidar M
    • Industrial Property Rights Holder
      Yokobayashi Y, Dwidar M
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Research-status Report
    • Overseas

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Published: 2018-07-25   Modified: 2022-01-27  

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