| Project/Area Number |
18KK0192
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 42:Veterinary medical science, animal science, and related fields
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| Research Institution | Gifu University |
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Principal Investigator |
Ito Naoto 岐阜大学, 応用生物科学部, 教授 (20334922)
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| Co-Investigator(Kenkyū-buntansha) |
山田 健太郎 宮崎大学, 農学部, 准教授 (70458280)
佐々木 道仁 北海道大学, 人獣共通感染症国際共同研究所, 講師 (70609403)
西山 祥子 岐阜大学, 応用生物科学部, 助教 (90817058)
西園 晃 大分大学, 医学部, 教授 (70218155)
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| Project Period (FY) |
2018-10-09 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2021: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 狂犬病ウイルス / 野外株 / 末梢感染 / P蛋白質 / 末梢感染機序 / インターフェロン / 筋肉細胞 |
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| Outline of Final Research Achievements |
In this study, with the goal of developing a prophylactic agent, which can supplement or replace rabies vaccine, we aimed to elucidate the importance and roles of rabies virus P protein isoforms P2-5 in the peripheral infection of the field strains We generated P2-5-deficient strains of the 1088 and Komatsugawa strains, respectively, by viral gene manipulation and examined their propagation and virulence (peripheral infectivity); the 1088-deficient strain showed decreased propagation and virulence, whereas the Komatsugawa-deficient strain showed no significant changes in these properties. Generation of the 1088 strain for in vivo imaging was attempted, but a significant decrease in its growth was observed due to an unexplained problem. Detailed examinations to optimize conditions for 3D tissue imaging using the uDISCO method also will be needed.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた成績は、今後、より詳細な検討が必要ではあるものの、狂犬病ウイルス野外株の病原性（末梢感染性）におけるP2-5アイソフォーム重要性はウイルス株ごとに異なることが示唆された。このことより、P2-5アイソフォームを標的として、どんなウイルス株にも対応可能な狂犬病予防薬を開発することは困難である可能性が高い。今後は、別のウイルス蛋白質を標的とした予防薬の開発にリソースを注ぐことが望ましいと判断できた。
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