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From toads to fireflies: Exploring the evolutionary transitional process of the prey-toxin resource in snakes

Research Project

Project/Area Number 18KK0205
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionKyoto University

Principal Investigator

Mori Akira  京都大学, 理学研究科, 教授 (80271005)

Co-Investigator(Kenkyū-buntansha) 森 直樹  京都大学, 農学研究科, 教授 (30293913)
城野 哲平  広島修道大学, 人間環境学部, 准教授 (70711951)
竹内 寛彦  日本大学, 生物資源科学部, 講師 (40726444)
江頭 幸士郎  北九州市立自然史・歴史博物館, 自然史課, 学芸員 (10738826)
Project Period (FY) 2018-10-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2021: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords防御行動 / ホタル毒 / 頸腺 / 進化 / 爬虫類 / 防御機構 / 毒 / 防御器官 / ヘビ / 餌毒利用 / ホタル
Outline of Final Research Achievements

Our molecular phylogenetic analysis revealed the presence of seven clades in the Rhabdophis nuchalis species group, and we described a new species in them. Based on the chemical characteristics of the nuchal gland fluids of these species, we found that some species mostly depend on toads as toxin source, others mostly depend on fireflies, and the others depend on both toads and fireflies. However, trophic skull morphology of them does not necessarily show features adapted to swallow the animals of the main toxin sources, indicating that dependency of toxin source on specific prey animals has not necessarily evolved along with the adaptive trophic morphology for them.

Academic Significance and Societal Importance of the Research Achievements

毒を持つ餌を食べて、その毒を自分の防御に再利用することが知られている動物は多くの分類群で知られている。ヤマカガシ属のヘビ類は頸腺という特殊な器官に餌から取り入れた毒を蓄積するが、進化の過程で毒源をヒキガエルからホタルへと大きく変化させた。このように餌源を分類学的にも生態学的にも大きくかけ離れた動物群に移行させた例は他の生物では知られていない。本研究は、そのような突飛な移行がどのような過程を経て進行してきたかを理解する手がかりを提供する。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (36 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (6 results) Journal Article (8 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (22 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Int'l Joint Research] 成都生物研究所/楽山師範大学(中国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] ユタ州立大学(米国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] 成都生物研究所/楽山師範大学(中国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] ユタ州立大学(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] 成都生物研究所/楽山師範大学(中国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] 成都生物研究所/楽山師範大学(中国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Cryptic diversity and phylogeography of the Rhabdophis nuchalis group (Squamata: Colubridae)2022

    • Author(s)
      Zhu Guangxiang、Yang Shijun、Savitzky Alan H.、Cheng Yuqi、Mori Akira、Ding Li、Rao Dingqi、Wang Qin
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 166 Pages: 107325-107325

    • DOI

      10.1016/j.ympev.2021.107325

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Do predators prefer toxic animals? A case of chemical discrimination by an Asian snake that sequesters firefly toxins2022

    • Author(s)
      Fukuda Masaya、Ujiie Rinako、Inoue Takato、Chen Qin、Cao Chengquan、Ding Li、Mori Naoki、Mori Akira
    • Journal Title

      Current Zoology

      Volume: 未定 Issue: 6 Pages: 1-8

    • DOI

      10.1093/cz/zoab102

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] New Insights Into Dietary Toxin Metabolism: Diversity in the Ability of the Natricine Snake Rhabdophis tigrinus to Convert Toad-Derived Bufadienolides2021

    • Author(s)
      Inoue Takato、Nakata Ryu、Savitzky Alan H.、Yoshinaga Naoko、Mori Akira、Mori Naoki
    • Journal Title

      Journal of Chemical Ecology

      Volume: 47 Issue: 10-11 Pages: 915-925

    • DOI

      10.1007/s10886-021-01287-6

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Does an Asian Natricine Snake, <i>Rhabdophis tigrinus</i>, Have Chemical Preference for a Skin Toxin of Toads?2021

    • Author(s)
      Fukuda, M. and Mori, A.
    • Journal Title

      Current Herpetology

      Volume: 40 Issue: 1 Pages: 1-9

    • DOI

      10.5358/hsj.40.1

    • NAID

      130007992258

    • ISSN
      1345-5834, 1881-1019
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] A New Species of the Genus Rhabdophis Fitzinger, 1843 (Squamata: Colubridae) in Southwestern Sichuan, China2020

    • Author(s)
      Piao, Y., Chen, Z., Wu, Y., Shi, S., Takeuchi, H., Jono, T., Fukuda, M., Mori, A., Tang, Y., Chen, Q., and Ding, L.
    • Journal Title

      Asian Herpetological Research

      Volume: 11 Pages: 95-107

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Variation in Bufadienolide Composition of Parotoid Gland Secretion From Three Taxa of Japanese Toads2020

    • Author(s)
      Inoue Takato、Nakata Ryu、Savitzky Alan H.、Yoshinaga Naoko、Mori Akira、Mori Naoki
    • Journal Title

      Journal of Chemical Ecology

      Volume: 46 Issue: 10 Pages: 997-1009

    • DOI

      10.1007/s10886-020-01217-y

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dramatic dietary shift maintains sequestered toxins in chemically defended snakes2020

    • Author(s)
      Tatsuya Yoshida, Rinako Ujiie, Alan H. Savitzky, Teppei Jono, Takato Inoue, Naoko Yoshinaga, Shunsuke Aburaya, Wataru Aoki, Hirohiko Takeuchi, Li Ding, Qin Chen, Chengquan Cao, Tein-Shun Tsai, Anslem de Silva, Dharshani Mahaulpatha, Tao Thien Nguyen, Yezhong Tang, Naoki Mori, and Akira Mori
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 11 Pages: 5964-5969

    • DOI

      10.1073/pnas.1919065117

    • NAID

      120006800720

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 生物が作り出す毒:どくどくしくない毒のはなし2020

    • Author(s)
      山下まり・此木敬一・稲垣英利・森 直樹・森 哲
    • Journal Title

      化学と生物

      Volume: 58 Pages: 111-119

    • NAID

      130007979405

    • Related Report
      2019 Research-status Report
  • [Presentation] ヤマカガシ属ヘビの保有する強心性ステロイド毒 (Bufadienolides) 組成の種間差2022

    • Author(s)
      井上綾、井上貴斗、森哲、森直樹
    • Organizer
      日本爬虫両生類学会第 61 回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ヤマカガシ属ヘビが保有する強心性ステロイドの種関差異2022

    • Author(s)
      井上 綾、井上 貴斗、吉永 直子、森 哲、森 直樹
    • Organizer
      日本農芸化学会 京都大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 毒源が変われば形態も変わる?ー食性の異なるヤマカガシ属における頭部形態の変化2022

    • Author(s)
      福田将矢、Jing-Song Shi、Alan H. Savitzky、Qin Chen、森哲
    • Organizer
      第69回日本生態学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 毒源が変われば形態も変わる?ー食性の異なるヤマカガシ属における頭部形態の変化2022

    • Author(s)
      井上貴斗, 中田隆, Alan H. Savitzky, 吉永直子, 森哲, 森直樹
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヤマカガシ属ヘビが保有する強心性ステロイドの種関差異2022

    • Author(s)
      井上綾、井上貴斗、吉永直子、森哲、森直樹
    • Organizer
      日本農芸化学会2022年大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヤマカガシにおけるエサ由来毒素変換能力の多様性2022

    • Author(s)
      井上貴斗、吉永直子、森哲、森直樹
    • Organizer
      日本農芸化学会2022年大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヤマカガシにおける「毒源生物」の化学認知に関する研究2021

    • Author(s)
      福田将矢、井上貴斗、森直樹、森哲
    • Organizer
      第40回日本動物行動学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヤマカガシにおける「毒源生物」の化学認知に関する研究2021

    • Author(s)
      福田将矢、井上貴斗、森直樹、森哲
    • Organizer
      日本爬虫両棲類学会第60回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] How predators recognize toxic animals as a toxin source: A case of chemical discrimination by an Asian snake that sequesters firefly toxins2021

    • Author(s)
      Masaya Fukuda, Qin Chen, Akira Mori
    • Organizer
      International Conference on Herpetological Biodiversity and Conservation in Eurasian Countries
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヤマカガシにおけるヒキガエル由来毒素変換能力の多様性2021

    • Author(s)
      井上貴斗, 中田隆, Alan H. Savitzky, 吉永直子, 森哲, 森直樹
    • Organizer
      日本農芸化学会 西日本・中四国・関西支部合同大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 日本産ヒキガエルが有する耳腺毒成分, bufadienolide の多様性2021

    • Author(s)
      井上貴斗、中田隆、サヴィツキイ・アラン、吉永直子、森哲、森直樹
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ヤマカガシ属ヘビの食性変化に伴う毒源のシフト2020

    • Author(s)
      吉田達哉、氏家里奈子、井上貴斗、城野哲平、福田将矢、森哲、森直樹
    • Organizer
      第62回天然有機化合物討論会
    • Related Report
      2020 Research-status Report
  • [Presentation] Toads or fireflies? Which do Asian natricine snakes prefer as a source of steroidal toxins?2020

    • Author(s)
      Fukuda M., Mori A., and Chen Q.
    • Organizer
      The 9th World Congress of Herpetology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regional characteristics of toxic components in Japanese toads2020

    • Author(s)
      Inoue Takato, Nakata Ryu, Ujiie Rinako, Yoshinaga Naoko, Savitzky Alan H., Mori Akira, and Mori Naoki
    • Organizer
      The 9th World Congress of Herpetology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヤマカガシ(Rhabdophis tigrinus) が有する頸腺毒成分の地域的特徴2020

    • Author(s)
      井上貴斗、中田隆、吉永直子、Alan H. Savitzky、森哲、森直樹
    • Organizer
      2020年度日本農芸化学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 中国産ヤマカガシ類の頸腺毒の由来および頭部の形態変化に関する考察2020

    • Author(s)
      福田将矢・井上貴斗・Alan Savitsky・Piao Yige・城野哲平・Chen Qin・森直樹・森哲
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Does an Asian natricine snake recognize fireflies that have cardiac steroidal toxins?2019

    • Author(s)
      Fukuda M., Mori A., and Chen Q.
    • Organizer
      The 36th International Ethological Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Unexpected origins of defensive compounds in animals2019

    • Author(s)
      Naoki MORI and Akira MORI
    • Organizer
      The 10th Conference of Asia-Pacific Association of Chemical Ecologists
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒキガエル属 (Bufo) が有する耳腺毒成分の地域的特徴2019

    • Author(s)
      井上貴斗、氏家里奈子、中田隆、吉永直子、Alan H. Savitzky、森哲、森直樹
    • Organizer
      2019 年度日本農芸化学会関西中部支部合同大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒキガエルかホタルか?中国産ヤマカガシ類の頸腺毒の由来に関する考察2019

    • Author(s)
      福田将矢・井上貴斗・Piao Yige・Chen Qin・森直樹・森哲
    • Organizer
      日本爬虫類両棲類学会第58回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヤマカガシ属ヘビ Rhabdophis pentasupralabialis が蓄積する毒性ステロイド bufadienolide の由来2019

    • Author(s)
      氏家 里奈子、竹内 寛彦、城野 哲平、チェン チン、ディン リー、タン イェチョン、ツァイ テインシュン、ツァオ チェンチャン、アラン サビツキー、油屋 駿介、青木 航、吉永 直子、森 哲、森 直樹
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒキガエルが持つステロイド毒 bufadienolide 類の化学分析2019

    • Author(s)
      井上 貴斗、氏家 里奈子、吉永 直子、森 哲、森 直樹
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report

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Published: 2018-10-12   Modified: 2024-01-30  

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