• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of virulent factor interactions of intestinal protozoa and assessment of lectin activity of Entamoeba histolytica lectin using a cell-based glycan array

Research Project

Project/Area Number 18KK0451
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))

Allocation TypeMulti-year Fund
Research Field Parasitology (including sanitary zoology)
Research InstitutionNagasaki University

Principal Investigator

Kato Kentaro  長崎大学, 熱帯医学研究所, 助教 (50508885)

Project Period (FY) 2019 – 2023
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Keywords赤痢アメーバ / Entamoeba histolytica / レクチン / Iglサブユニット / Hglサブユニット / エクソソーム / 寄生虫学 / 糖鎖生物学 / 糖鎖アレイ
Outline of Final Research Achievements

In this project, I tried to identify virulence factors that interact with Entamoeba histolytica Igl lectin subunits. The virulence factors that interacted with Igl could not be identified due to the amount even though an interaction between Igl and another lectin subunit, Hgl, was confirmed by western blotting. In the process, I found that Igl fragments were in exosome fractions from E. histolytica culture supernatant and Igl had three regions for its hemolytic and cytotoxic activities.
Igl is known to have a very weak affinities toward glycans. Therefore, I tried to identify the glycans recognized by Igl by using "Cell-based glycan array". As the results, Igl showed non-specific binding to the cells that had different glycans on the cell surfaces and I could not clarify the glycans that specifically recognized by Igl.
I found that rare sugars could inhibit the growth of E. histolytica trophozoites in vitro.

Academic Significance and Societal Importance of the Research Achievements

赤痢アメーバIglレクチンサブユニットと相互作用する病原性因子の存在がわかり、この病原性因子を明らかにすることで、赤痢アメーバが病原性を発現する機構が明らかとなる。Iglの断片がエクソソーム中に存在することが示唆され、赤痢アメーバの病原性発現へのIglの関与が考えられる。Iglには溶血および細胞傷害性を有する領域が複数あり、Iglの活性を抑制するためには、すべての領域を阻害する必要があることを示せた。
Iglは細胞への非特異的吸着が強く、Iglの糖鎖親和性を明らかにするには別の方法が必要であることが示された。
赤痢アメーバの増殖を希少糖が抑制し、このことは赤痢アメーバ症への新薬開発に繋がる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (13 results)

All 2023 2022 2021 2019

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] コペンハーゲン大学(デンマーク)2019

    • Year and Date
      2019-05-15
    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] バージニア大学(米国)2019

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Effects of monosaccharides including rare sugars on proliferation of Entamoeba histolytica trophozoites in vitro2023

    • Author(s)
      Kato Kentaro、Miura Mitsumasa、Tachibana Hiroshi、Tsukamoto Ikuko
    • Journal Title

      Frontiers in Molecular Biosciences

      Volume: 10 Pages: 1288470-1288470

    • DOI

      10.3389/fmolb.2023.1288470

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Multiple Domains of Entamoeba histolytica Intermediate Subunit Lectin-1 with Hemolytic and Cytotoxic Activities2022

    • Author(s)
      Kato Kentaro、Tachibana Hiroshi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 Issue: 14 Pages: 7700-7700

    • DOI

      10.3390/ijms23147700

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 赤痢アメーバレクチンのIglサブユニットは界面活性を有している2023

    • Author(s)
      加藤健太郎, 新地浩之, 隅田泰生, 橘裕司
    • Organizer
      第42回日本糖質学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Function and localization of intermediate subunit of Entamoeba histolytica lectin2023

    • Author(s)
      Kentaro Kato, Takashi Makiuchi, Hiroyuki Shinchi, Audrey C. Brown, Yasuo Suda, Hiroshi Tachibana, William A. Petri Jr.
    • Organizer
      XX International Seminar on Amebiasis 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 寄生虫感染における糖鎖関連分子の役割2023

    • Author(s)
      加藤 健太郎
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 赤痢アメーバIgl1レクチンは溶血および細胞傷害活性領域を複数有する2023

    • Author(s)
      加藤 健太郎、橘 裕司
    • Organizer
      第4回卓越大学院プログラム日英グローバルヘルスシンポジウム
    • Related Report
      2022 Research-status Report
  • [Presentation] 赤痢アメーバIgl1レクチンは溶血および細胞傷害活性領域を複数有する2022

    • Author(s)
      加藤 健太郎、橘、裕司
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] Entamoeba histolytica Igl1レクチンの分子サイズは培養条件により異なる2021

    • Author(s)
      加藤 健太郎、花松 久寿、古川 潤一、畠山 智充、橘 裕司
    • Organizer
      第40回日本糖質学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 腸管寄生原虫Entamoeba histolytica のIgl1 レクチンの分子サイズは培養条件により異なる2021

    • Author(s)
      加藤 健太郎、花松 久寿、古川 潤一、畠山 智充、橘 裕司
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 赤痢アメーバIglレクチンの溶血活性領域の同定2019

    • Author(s)
      加藤健太郎、牧内貴志、橘裕司
    • Organizer
      第38回日本糖質学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Beyond antibiotics--赤痢アメーバ感染制御に向けた生物学2019

    • Author(s)
      加藤健太郎
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
    • Invited

URL: 

Published: 2019-02-06   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi