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Elucidation of the nitrogen-fixing endophyte in yam and agricultural innovation

Research Project

Project/Area Number 18KT0046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Agricultural Resources for the Next Generation
Research InstitutionTokyo University of Agriculture

Principal Investigator

Shiwachi Hironobu  東京農業大学, 国際食料情報学部, 教授 (40385505)

Co-Investigator(Kenkyū-buntansha) 朴 炳宰  鹿児島大学, 農水産獣医学域農学系, 准教授 (00538879)
遠城 道雄  鹿児島大学, 農水産獣医学域農学系, 教授 (60194651)
Project Period (FY) 2018-07-18 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywords作物学 / 育種学 / ヤムイモ / 栽培技術 / 施肥技術 / 窒素固定細菌 / 植物生育促進細菌 / 微生物工学 / イネ / バイオインフォマティック
Outline of Final Research Achievements

We aimed to develop a crop cultivation technique using nitrogen-fixing bacteria (NFB) isolated from yams. In this study, 22 species of NFB of 18 genera were isolated from yams. The isolated NFB Rhizobium sp. was presumed to be a new species specialized for endogenous to yams. As a result of inoculating the isolated Agrobacterium sp. To a water yam (a kind of yam), the weight of the tuber and the nitrogen content of the leaves increased. As a result of the meta 16S analysis of the bacterium, the bacterium of the yam was mainly Proteobacteria of 5 genera. Proteobacteria is might related with yam growth. Furthermore, when upland rice was inoculated with each of the five types of bacteria, some bacteria had the effect of promoting growth.

Academic Significance and Societal Importance of the Research Achievements

ヤムイモに多くの種類の窒素固定細菌が共生することはこれまでに報告がなかった。本研究で得られた成果は細菌と共生する作物の特徴の解明や細菌を利用した新しい畑作の肥培管理技術の開発につながると期待された。特に、植物生育促進細菌や窒素固定エンドファイトを利用した作物栽培システムの開発は経済的に化学肥料投入が困難な開発途上国において重要になると考えられる。現在、これらの細菌の分離・同定並びに機能性の研究はアフリカのナイジェリアの国際熱帯研究所に技術移転しており、細菌利用の研究が開始されている。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (29 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 10 results) Presentation (11 results) (of which Int'l Joint Research: 4 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] 国際熱帯農業研究所(IITA)(ナイジェリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 国際熱帯農業研究所(IITA)(ナイジェリア)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] 国際熱帯農業研究所(IITA)(ナイジェリア)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Bacterial Community of Water Yam (<i>Dioscorea alata</i> L.) cv. A-192022

    • Author(s)
      1.Kihara, Shunta, Yamamoto Kosuke, Hisatomi Atsushi, Shiwa Yuh, Chu Chia-Cheng, Takada Kanako, Ouyabe Michel, Pachakkil Babil, Kikuno Hidehiko, Tanaka Naoto, Shiwachi Hironobu
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME21062

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Growth of Water Yam (Dioscorea alata L.) as Affected by Bacterial Inoculation under Growth Chamber Conditions.2021

    • Author(s)
      Liswadiratanakul Sumetee, Michel Ouyabe, Hideki Hinomizu, Kanako Takada, Hidehiko Kikuno, Babil Pachakkil and Hironobu Shiwachi
    • Journal Title

      Tropical Agriculture and Development

      Volume: 65 Pages: 41-48

    • NAID

      130008065605

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rhizobium dioscoreae sp. nov., a plant growth-promoting bacterium isolated from yam (Dioscorea species)2020

    • Author(s)
      Michel Ouyabe, Naoto Tanaka, Yuh Shiwa, Nobuyuki Fujita, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: ー Issue: 9 Pages: 5054-5062

    • DOI

      10.1099/ijsem.0.004381

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Response of upland rice (Oryza sativa L.) inoculated with non-native plant growth-promoting bacteria2020

    • Author(s)
      Michel Ouyabe, Irie Kenji, Tanaka Naoto, Hidehiko Kikuno, Babil Pachakkil and Hironobu Shiwachi
    • Journal Title

      Agronomy

      Volume: 10 Issue: 6 Pages: 903-903

    • DOI

      10.3390/agronomy10060903

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Infection Route of Endophytic Nitrogen-fixing Bacteria in Water Yam (<i>Dioscorea alata</i> L.)2020

    • Author(s)
      Hironobu Shiwachi, Natsumi Miwa, Kanako Takada, Michel Ouyabe, Babil Pachakkil, Hidehiko Kikuno, and Naoto Tanaka
    • Journal Title

      Tropical Agriculture and Development

      Volume: 64 Issue: 1 Pages: 1-6

    • DOI

      10.11248/jsta.64.1

    • NAID

      130007869005

    • ISSN
      1882-8450, 1882-8469
    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrogen-Fixing Bacteria Associated with Yams (Dioscorea spp.) Growth2019

    • Author(s)
      Hironobu SHIWACHI, Hidehiko KIKUNO Pachakkil BABIL, Michel OUYABE, Kanako TAKADA, Natsumi MIWA and Naoto TANAKA
    • Journal Title

      熱帯農業研究

      Volume: 12 Pages: 121-122

    • NAID

      130007868952

    • Related Report
      2019 Research-status Report
  • [Journal Article] Isolation of Nitrogen-Fixing Bacteria from Water Yam (Dioscorea alata L.)2019

    • Author(s)
      Kanako TAKADA, Naoto TANAKA, Hidehiko KIKUNO, Pachakkil BABIL, Michio ONJO, Byoung-Jae PARK, and Hironobu SHIWACHI
    • Journal Title

      Tropical Agriculture and Development

      Volume: 63 Pages: 198-203

    • NAID

      130007898141

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contribution of Biological Nitrogen Fixation in Lesser Yam (<i>Dioscorea esculenta</i> L.) Associated with Endophytic Diazotrophic Bacteria2019

    • Author(s)
      Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, and Hironobu SHIWACHI
    • Journal Title

      Tropical Agriculture and Development

      Volume: 63 Issue: 3 Pages: 131-139

    • DOI

      10.11248/jsta.63.131

    • ISSN
      1882-8450, 1882-8469
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endophytic Nitrogen-Fixing Bacteria of Water Yam (<i>Dioscorea alata</i> L.) in Relation with Fertilization Practices2019

    • Author(s)
      Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL, and Hironobu SHIWACHI
    • Journal Title

      Tropical Agriculture and Development

      Volume: 63 Issue: 3 Pages: 122-130

    • DOI

      10.11248/jsta.63.122

    • ISSN
      1882-8450, 1882-8469
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation and identification of nitrogen-fixing bacteria associated with Dioscorea alata L. and Dioscorea esculenta L.2019

    • Author(s)
      Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL and Hironobu SHIWACHI
    • Journal Title

      Microbial Resources and Systematics

      Volume: 35 Pages: 3-11

    • NAID

      40022001014

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of the Source of Nitrogen During Water Yam (<i>Dioscorea alata</i> L.) Growth Using δ<sup>15</sup>N Observations2018

    • Author(s)
      Kanako Takada, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
    • Journal Title

      Tropical Agriculture and Development

      Volume: 62 Issue: 3 Pages: 124-131

    • DOI

      10.11248/jsta.62.124

    • NAID

      130007497597

    • ISSN
      1882-8450, 1882-8469
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ヤムイモ(Dioscorea spp.)から分離された細菌の植物生育促進特性2021

    • Author(s)
      生川友亮、Ouyabe Michel、高田花奈子、Liswadiratanakul Sumetee、田中尚人、松本亮、志和地弘信
    • Organizer
      日本熱帯農業学会第130回講演会(オンライン)2021年11月13日
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ダイジョ(Dioscorea alata L.)から分離された窒素固定細菌Agrobacterium sp.の作物への感染機構2021

    • Author(s)
      森巧大、山本絋輔、Ouyabe Michel、田中尚人、志和地弘信
    • Organizer
      日本熱帯農業学会第130回講演会(オンライン).2021年11月13日
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metagenomic Analysis of Nitrogen-Fixing Bacteria in Six Accessions of White Guinea Yam (Dioscorea rotundata Poir.)2020

    • Author(s)
      Ayodeji Peter Idowu, Kosuke Yamamoto, Yuh Shiwa, Minenosuke Matsutani, Kanako Takada, Michel Ouyabe, Babil Pachakkil, Hidehiko Kikuno, Asrat Asfaw, Ryo Matsumoto and Hironobu Shiwachi
    • Organizer
      日本熱帯農業学会第128回講演会(オンライン)
    • Related Report
      2020 Research-status Report
  • [Presentation] ダイジョ(Dioscorea alata L.)における共生細菌叢のメタ16S 解析2020

    • Author(s)
      木原駿太、山本絋輔、志波優、朱 家成、髙田花奈子、Ouyabe Michel、パチャキル バビル、菊野日出彦、田中尚人、志和地弘信
    • Organizer
      日本熱帯農業学会第128回講演会(オンライン)
    • Related Report
      2020 Research-status Report
  • [Presentation] 15N希釈法によるホワイトギニアヤムの窒素固定量の推定2020

    • Author(s)
      髙田 花奈子・松本 亮・石川 春樹・パチャキル バビル・菊野 日出彦・志和地 弘信
    • Organizer
      日本作物学会第249回講演会 筑波産学連携支援センター(つくば市)
    • Related Report
      2019 Research-status Report
  • [Presentation] Effects of Agrobacterium sp. Strain 343 Inoculation on the Growth of Water Yam (Dioscorea alata L.) under Greenhouse Conditions2020

    • Author(s)
      Sumetee Liswadiratanakul, Michel Ouyabe, Kodai Mori, Kanako Takada, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
    • Organizer
      日本熱帯農業学会第127回講演会(東京農工大学府中キャンパス)
    • Related Report
      2019 Research-status Report
  • [Presentation] Possibility of nitrogen fixing to white yam (Dioscorea rotundata) using Natural abundance of 15N2019

    • Author(s)
      Kanako Takada, Ryo Matsumoto, Haruki Ishikawa, Asrat AMELE, Babil PACHAKKIL, Hidehiko Kikuno and Hironobu Shiwachi
    • Organizer
      Tropentag 2019 (Universities of Kassel and Goettingen、ドイツ)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of Isolated Bacterial Inoculation on Water Yam (Dioscorea alata L.) as a Plant Growth Promoting Bacteria2019

    • Author(s)
      Sumetee Liswadiratanakul, Michel Ouyabe, Hideki Hinomisu, Kanako Takada, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
    • Organizer
      日本熱帯農業学会第126回講演会(アトールエメラルド宮古島)
    • Related Report
      2019 Research-status Report
  • [Presentation] メタ16S解析法によるダイジョ共生細菌叢の解明2019

    • Author(s)
      朱 家成、山本絋輔、志波優、Michel Ouyabe、高田花奈子、パチャキル バビル、菊野日出彦、田中尚人、志和地弘信
    • Organizer
      日本熱帯農業学会第126回講演会(アトールエメラルド宮古島)
    • Related Report
      2019 Research-status Report
  • [Presentation] Biological Nitrogen Fixation in Water Yam (Dioscorea alata L.) and Lesser Yam (Dioscorea esculenta L.) Associated with Endophytic Diazotrophic Bacteria2019

    • Author(s)
      Michel OUYABE, Hidehiko KIKUNO, Naoto TANAKA, Pachakkil BABIL and Hironobu SHIWACHI
    • Organizer
      日本熱帯農業学会第125回講演会 (千葉大学)
    • Related Report
      2018 Research-status Report
  • [Presentation] Diversity of Nitrogen-fixing bacteria associated with yams (Dioscorea spp.)2018

    • Author(s)
      Michel Ouyabe, Hidehiko Kikuno, Naoto Tanaka, Pachakkil Babil, Hironobu Shiwachi
    • Organizer
      Tropentag 2018 (ゲント大学、ベルギー)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] ヤムイモの窒素固定エンドファイトの解明と農業革新

    • URL

      https://www.nodai.ac.jp/academics/int/int/sdgs/03/

    • Related Report
      2021 Annual Research Report 2020 Research-status Report 2019 Research-status Report 2018 Research-status Report
  • [Remarks] 植物成長促進・窒素固定細菌で農業革新

    • URL

      https://www.youtube.com/watch?v=itbyy2ST8Dw

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
  • [Patent(Industrial Property Rights)] 新規窒素固定細菌、植物成長促進剤及び植物の栽培方法2019

    • Inventor(s)
      志和地弘信、田中尚 人、菊野日出彦、パ チャキルバビル
    • Industrial Property Rights Holder
      東京農業大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-209500
    • Filing Date
      2019
    • Related Report
      2020 Research-status Report
  • [Patent(Industrial Property Rights)] 新規窒素固定細菌、植物成長促進剤及び植物の栽培方法2019

    • Inventor(s)
      志和地弘信、田中尚人、菊野日出彦、パチャキルバビル
    • Industrial Property Rights Holder
      東京農業大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-209500
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2018-07-20   Modified: 2023-01-30  

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