Higher-order genomic structure and subcellular organization

• Project/Area Number: 19207001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyoto University
• Principal Investigator: TAKEYASU Kunio Kyoto University, 大学院・生命科学研究科, 教授 (40135695)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Shigehiro 京都大学, 大学院・生命科学研究科, 准教授 (90346106) ; HIDUME Kouji 京都大学, 大学院・生命科学研究科, 助教 (10378846) ; ATOMI Haruyuki 京都大学, 大学院・工学科学研究科, 准教授 (90243047) ; 小堀 俊郎 食品総合研究所, 食品工学研究領域, 研究員 (10353971)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥51,480,000 (Direct Cost: ¥39,600,000、Indirect Cost: ¥11,880,000); Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2008: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2007: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
• Keywords: ゲノム構築 / 機能 / 再編 / 発現 / 維持

Research Abstract

(1) In Archaea, TK0471 is a structural protein as well as a transcription factor. (2) Nucleolar proteins (MAk16, WD46, MAGEFF, FAM27E1, etc.) with unknown function move very slowly compared to the well-known nucleolar proteins. They behave also very differently in the presence of transcription inhibitor, actinomycin D. (3) A cytoskeletal protein, actinin-4, shuttles between cytoplasm and nuclelar plasm, dependent of cell cycle and independent on importin-β.

Research Products

• [Journal Article] Motion of Ca-pump captured. (2011)
  • Author(s): M.Yokokawa, K.Takeyasu
  • Journal Title: FEBS J. In Press
• [Journal Article] Unusual structures are present in DNA fragments containing super-long Huntingtin CAG repeats. (2011)
  • Author(s): Daniel Duzdevich, Jinliang Li, Jhoon Whang, H.Takahashi, K.Takeyasu, David T.F. Dryden, A.Jennifer Morton, J.Michael Edwardson
  • Journal Title: PloS ONE 6
• [Journal Article] Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesise. (2011)
  • Author(s): Muehiro Asally, Y.Yasuda, M.Oka, S.Otsuka S.H.Y oshimura, Ktakeyasu, Y.Yoneda
  • Journal Title: The FASEB J. 278 Pages: 610-621
• [Journal Article] RecO-mediated DNA homology search and annealing is facilitated by SsbA. (2011)
  • Author(s): K.Takeyasu, 他3名
  • Journal Title: Nucleic Acids Res. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Single-molecule Anatomy by Atomic Force Microscopy and Recognition Imaging. (2011)
  • Author(s): K.Takeyasu, 他4名
  • Journal Title: Arch.Histology and Cytology Volume: (印刷中)
  • NAID: 10028159728
  • Peer Reviewed
• [Journal Article] Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis. (2010)
  • Author(s): H.Maruyama, Minsang Shin , T.Oda , Rie Matsumi , R.L.Ohniwa , T.Itoh , K.Shirahige , T.Imanaka , H.Atomi , Shige H.Yoshimura , K.Takeyasu
  • Journal Title: Mol.Biol.Cell 22 Pages: 386-398
  • NAID: 120002871160
• [Journal Article] Formation of equilateral-triangular shaped RNA-protein complex with kink-turn-L7Ae interacting motifs. (2010)
  • Author(s): H.Ohno, T.Kobayashi, R.Kabata, T.Iwasa, S.H.Yoshimura, K.Takeyasu, T.Inoue, H.Saito
  • Journal Title: Nature Nanotechnology
• [Journal Article] Molecular Dynamics of DNA and Nucleosomes in Solution Studied by Fast-scanning Atomic Force Microscopy. (2010)
  • Author(s): Y.Suzuki, Y.Higuchi, K.Hizume, M.Yokokawa, S.H.Yoshimura, K.Yoshikawa, K.Takeyasu
  • Journal Title: Ultramicroscopy 110 Pages: 682-688
• [Journal Article] RecO-mediated DNA homology search and annealing is facilitated by SsbA. (2010)
  • Author(s): C.Manfredi, Y.Suzuki, T.Yadav, K.Takeyasu, J.C.Alonso.
  • Journal Title: Nucleic Acids Res. 38 Pages: 6920-6929
• [Journal Article] Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. (2010)
  • Author(s): M.Kumeta, S.H.Yoshimura, M.Harata, K.Takeyasu
  • Journal Title: J.Cell Sci. 123 Pages: 1020-1030
  • NAID: 120002561115
• [Journal Article] Interaction of synaptotagmin with lipid bilayers, analyzed by single-molecule force spectroscopy. (2010)
  • Author(s): H.Takahashi, Victor Shahin, Robert M.Henderson, K.Takeyasu, J.Michael Edwardson
  • Journal Title: Biophysical J. 99 Pages: 2550-2558
• [Journal Article] Acid-sensing ion channel (ASIC) 1a undergoes a height transition in response to acidification. (2010)
  • Author(s): M.Yokokawa, K.Takeyasu, J.M.Edwardson
  • Journal Title: FEBS Lett. 584 Pages: 3107-3110
• [Journal Article] Adaptation beyond the Stress Response : Cell Structure Dynamics and Population Heterogeneity in Staphylococcus aureus (2010)
  • Author(s): K.Morikawa, R.L.Ohniwa, T.Ohta, Y.Tanaka, K.Takeyasu, T.Msadek
  • Journal Title: Microbes and Environments 25 Pages: 75-82
  • NAID: 10030688947
• [Journal Article] Molecular mechanisms underlying nucleocytoplasmic shuttling of actin in-4. (2010)
  • Author(s): K.Takeyasu, 他2名
  • Journal Title: J.Cell Sci. Volume: 123 Pages: 1020-1030
  • Peer Reviewed
• [Journal Article] A Novel Nucleoid Protein of Escherichia coli Induced Under Anaerobiotic Growth Conditions. (2010)
  • Author(s): K.Takeyasu, 他2名
  • Journal Title: Nucleic Acid Res. Volume: 38 Pages: 3605-3618
  • Peer Reviewed
• [Journal Article] Molecular Dynamics of DNA and Nucleosomes in Solution Studied by Fast-scanning Atomic Force Microscopy. (2010)
  • Author(s): K.Takeyasu, 他6名
  • Journal Title: Ultramicroscopy Volume: 110 Pages: 682-688
  • Peer Reviewed
• [Journal Article] Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis. (2010)
  • Author(s): K.Takeyasu, 他10名
  • Journal Title: Mol.Biol.Cell Volume: 22 Pages: 386-398
  • NAID: 120002871160
  • Peer Reviewed
• [Journal Article] Interaction of synaptotagmin with lipid bilayers, analyzed by single-molecule force spectroscopy. (2010)
  • Author(s): K.Takeyasu, 他3名
  • Journal Title: Biophysical J. Volume: 99 Pages: 2550-2558
  • Peer Reviewed
• [Journal Article] Molecular Dynamics of DNA and Nucleosomes in Solution Studied by Fast-scanning Atomic Force Microscopy (2010)
  • Author(s): Y. Suzuki, Y Higuchi, K. Hizume, M. Yokokawa, S. H. Yoshimura, K. Yoshikawa, K. Takeyuasu
  • Journal Title: Ultramicroscopy (In Press)
  • Peer Reviewed
• [Journal Article] Single-molecule Anatomy by Atomic Force Microscopy and Recognition Imaging (2010)
  • Author(s): H. Takahashi, K. Hizume, M. Kumeta, S. H. Yoshimura, K. Takeyasu
  • Journal Title: Arch. Histology and Cytology (In Press)
  • NAID: 10028159728
  • Peer Reviewed
• [Journal Article] Single-molecule Anatomy by Atomic Force Microscopy and Recognition Imaging. (2009)
  • Author(s): H.Takahashi, K.Hizume, M.Kumeta, S.H.Yoshimura, K.Takeyasu
  • Journal Title: Arch.Histology and Cytology 72 Pages: 17-225
  • NAID: 10028159728
• [Journal Article] Cell Cycle-Dependent Phosphorylation of MAN-1. (2009)
  • Author(s): Y.Hirano, Y.Iwase, K.Ishii, M.Kumeta, T.Horigome, K.Takeyasu
  • Journal Title: Biochemistry 48 Pages: 1636-1643
• [Journal Article] Removal of histone tails from nucleosome dissects the physical mechanisms of salt-induced aggregation, linker histone H1-induced compaction and 30-nm fiber formation of the nucleosome array. (2009)
  • Author(s): K.Hizume, T.Nakai, S.Araki, E.Prieto, K.Yoshikawa, K.Takeyasu
  • Journal Title: Ultramicroscopy 109 Pages: 868-873
  • NAID: 120001468043
• [Journal Article] Single-Molecule Dynamics of the DNA-EcoRII Protein Complexes Revealed with High-Speed Atomic Force Microscopy. (2009)
  • Author(s): J.L.Gilmore, Y.Suzuki, G.Tamulaitis, V.Siksnys, K.Takeyasu, Y.L.Lyubchenko
  • Journal Title: Biochemistry. 48(44) Pages: 10492-10498
• [Journal Article] Nucleosomal arrays reconstituted from ring and linear DNA. (2009)
  • Author(s): S.Araki, K.Hizume, T.Iwaki, Y.Suzuki, K.Takeyasu, K.Yoshikawa
  • Journal Title: Chem.Phys.Lett. 479 Pages: 284-289
• [Journal Article] Single-molecule analysis of protein-DNA complexes formed during partition of newly replicated plasmid molecules in Streptococcus pyogenes. (2009)
  • Author(s): F.Pratto, Y.Suzuki, K.Takeyasu, J.C.Alonso
  • Journal Title: J Biol Chem. 284(44) Pages: 30298-30306
• [Journal Article] Cell Cycle-Dependent Phosphorylation of MAN-1 (2009)
  • Author(s): Y. Hirano, Y. Iwase, K. Ishii, M. Kumeta, T. Horigome, K. Takeyasu
  • Journal Title: Biochemistry 48 Pages: 1636-1643
  • Peer Reviewed
• [Journal Article] Removal of histone tails from nucleosome dissects the physical mechanisms of salt-induced aggregation, linker histone H1-induced compaction and 30-nm fiber formation of the nucleosome array (2009)
  • Author(s): K. Hizume, T. Nakai, S. Araki, E. Prieto, K. Yoshikawa, K. Takeyasu
  • Journal Title: Ultramicroscopy 109 Pages: 868-873
  • NAID: 120001468043
  • Peer Reviewed
• [Journal Article] Single-Molecule Dynamics of the DNA-EcoRII Protein Complexes Revealed with High-Speed Atomic Force Microscopy (2009)
  • Author(s): J. L. Gilmore, Y. Suzuki, G. Tamulaitis, V. Siksnys, K. Takes, Y. L. Lyubchenko
  • Journal Title: Biochemistry 48 (44) Pages: 10492-8
  • Peer Reviewed
• [Journal Article] Single-molecule analysis of protein-DNA complexes formed during partition of newly replicated plasmid molecules in Streptococcus pyogenes (2009)
  • Author(s): F. Pratto, Y. Suzuki, K. Takeyasu, J. C. Alonso
  • Journal Title: J Biol Chem. 284 (44) Pages: 30298-306
  • Peer Reviewed
• [Journal Article] Nucleosomal arrays reconstituted from ring and linear DNA (2009)
  • Author(s): K.Takeyasu, 他5名
  • Journal Title: Chem.Phys.Letter Volume: 479 Pages: 284-289
  • Peer Reviewed
• [Journal Article] Single molecure dynamics of the EcoRII protein revealed with high-speed Atomic Force Microscopy (2009)
  • Author(s): K.Takeyasu, 他5名
  • Journal Title: Biochemisitry Volume: 48 Pages: 10492-10498
  • Peer Reviewed
• [Journal Article] Cell Cycle-Dependent Phosphorylation of MAN-1 (2009)
  • Author(s): K.Takeyasu, 他5名
  • Journal Title: Biochemistry Volume: 48 Pages: 1636-1643
  • Peer Reviewed
• [Journal Article] Proteomic and targeted analytical identification of BXDC1 and EBNA1BP2 as dynamic scaffold proteins in the nucleous. (2008)
  • Author(s): Y.Hirano, K.Ishii, M.Kumeta, K.Furukawa, K.Takeyasu, T.Horigome.
  • Journal Title: Genes Cell 14 Pages: 155-166
• [Journal Article] Nuclear matrix contains novel WD-repeat and disordered-regin-rich proteins. (2008)
  • Author(s): K.Ishii, Y.Hirano, N.Araki, T.Oda, M.Kumeta, K.Takeyasu, K.Furukawa, T.Horigome
  • Journal Title: FEBS Lett. 582 Pages: 3515-3519
• [Journal Article] Individual binding pockets of importin beta for FG-nucleoporins have different binding properties and different sensitivities to RanGTP. (2008)
  • Author(s): S.Otsuka, S.Iwasaka, Y.Yoneda, H.Takahashi, K.Takeyasu, S.H.Yoshimura
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 16101-16106
• [Journal Article] Scanning probe microscope revealed mechanical properties of plasma membrane and nuclear envelope in living cells. (2008)
  • Author(s): M.Yokokawa, K.Takeyasu, S.H.Yoshimura
  • Journal Title: J.Microscopy 232 Pages: 82-90
• [Journal Article] Nuclear Architectures and Chromatin Dynamics Revealed by Atomic Force Microscopy in Combination with Biochemistry and Cell Biology. (2008)
  • Author(s): Y.Hirano, H.Takahashi, M.Kumeta, K,Hizume, Y.Hirai, S.Otsuka, S.H.Yoshimura, K.Takeyasu
  • Journal Title: Eur.J.Physiol. 456 Pages: 139-153
• [Journal Article] Nuclear Architectures and Chromatin Dynamics Revealed by Atomic Force Microscopy in Combination with Biochemisitry and Cell Buology (2008)
  • Author(s): K. Hizume, S. H. Yoshimura, K. takeyasu
  • Journal Title: Eur. J. Physiology 456 Pages: 139-153
  • Peer Reviewed
• [Journal Article] In situ analysisi of the higher-order genome strucuturein a single Escherichis coli cell. (2008)
  • Author(s): K. Takeyasu
  • Journal Title: J. Biotechnology 133 Pages: 172-176
  • Peer Reviewed
• [Journal Article] Scanning probe microscope revealed mechanical properties of plasma membrance and nuclear in living cells (2008)
  • Author(s): K. Takeyasu, S. H. Yoashimura
  • Journal Title: J. Microscopy 232 Pages: 82-90
  • Peer Reviewed
• [Journal Article] In situ analysis of the higher-order genome structure in a single Escherichia coli cell. (2008)
  • Author(s): E. Shindo, K. Kubo, R.L. Ohniwa, K. Takeyasu & K. Yoshikawa.
  • Journal Title: J. Biotechnology. 133(印刷中)
  • Peer Reviewed
• [Journal Article] Transcription-coupled uncleoid architecture in bacteria. (2007)
  • Author(s): R.L.Ohniwa, K.Morikawa, S.L.Takeshita, J.Kim, T.Ohta, C.Wada, K.Takeyasu
  • Journal Title: Gene to Cells 12 Pages: 1141-1152
• [Journal Article] Fast-scan atomic force microscopy reveals that the type III restriction enzyme EcoP15I is capable of DNA translocation and looping. (2007)
  • Author(s): N.Crampton, M.Yokokawa, D.T.Dryden, J.M.Edwardson, D.N.Rao, K.Takeyasu, S.H.Yoshimura, R.M.Henderson
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 12755-12760
• [Journal Article] Topoisomerase II, a scaffold component, promotes chromatin-compaction in vitro in a linker-histone H1-dependent manner. (2007)
  • Author(s): K.Hizume, S.Araki, K.Yoshikawa, K.Takeyasu
  • Journal Title: Nucleic Asid Res. 35 Pages: 2787-2799
  • NAID: 120000896374
• [Journal Article] Chromatin dynamics of unfolding and refolding controlled by the nucleosome repeat length and the linker and core histones. (2007)
  • Author(s): T.Kobori, S.Iwamoto, K.Takeyasu, T.Ohtani
  • Journal Title: Biopolymers 85 Pages: 295-307
• [Journal Article] Dynamic structures of Bacillus subtillis RecN-DNA complex. (2007)
  • Author(s): H. Sanchez, P.P. Cardenas, S.H. Yoshimura, K.Takeyasu & J.C. Alonso.
  • Journal Title: Nucleic Acid Res. 36 Pages: 110-120
  • Peer Reviewed
• [Journal Article] Transcription-coupled Nucleoid Architecture in Bacteria. (2007)
  • Author(s): R.L. Ohniwa, K. Morikawa, J. Kim, S.L. Takeshita, T. Ohta, C. Wada & K. Takeyasu.
  • Journal Title: Gene to Cells. 12 Pages: 1141-1152
  • Peer Reviewed
• [Presentation] Karyopherin-independent nuclear Transport of spectrin for superfamily proteins (2010)
  • Author(s): M.Kumeta, S.Otsuka, S.H.Yoshimura, K.Takeyasu
  • Organizer: The American Society for Cell Biology-50^<th> annual meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2010-12-14
• [Presentation] Karyopherin-independent nuclear transport of spectrin superfamily proteins (2010)
  • Author(s): K.Takeyasu, 他3名
  • Organizer: 50^<th> ASCB Annual Meeting
  • Place of Presentation: Philadelphia(アメリカ)
  • Year and Date: 2010-12-14
• [Presentation] Nucleosome and Chromatin Dynamics Revealed by Atomic Force Microscopy (2009)
  • Author(s): K.Takeyasu
  • Organizer: The 7th European Biophysical Society Association meeting
  • Place of Presentation: Genoa, Italy
  • Year and Date: 2009-07-15
• [Presentation] Nucleosome and Chromatin Dynamaics Revealed by Atomic Force Microscopy. (2009)
  • Author(s): K.Takeyasu
  • Organizer: The 7^<th> European Biophysical society Association meeting
  • Place of Presentation: Genoa(イタリア)
  • Year and Date: 2009-07-12
• [Presentation] Genome/Chromatin Dynamics Revealed by Atomic force Microscopy (2008)
  • Author(s): K.Takeyasu
  • Organizer: International Scanning Probe Microscopy conference
  • Place of Presentation: Seattle, USA
  • Year and Date: 2008-06-22
• [Presentation] Genome/Chromatin Dynamics Revealed by Atomic force Microscopy (2008)
  • Author(s): Kunio Takeyasu
  • Organizer: International Scanning Probe Microscopy conference 2008
  • Place of Presentation: Seattle, USA
  • Year and Date: 2008-06-22
• [Presentation] Nano-scale imaging and single molecure manipulation techniques for uncovering the nuclear architectures and dyanamics (2008)
  • Author(s): Takeyasu Kunio
  • Organizer: The 10th Asian Congress On Transcription Meeting
  • Place of Presentation: バンガロール(インド)
  • Year and Date: 2008-01-14
• [Book] Scanning Probe Microscopy in Nanoscience and Nanotechnology, Vol 2(Biological Application of Fast-Scanning Atomic Force Microscopy)(ed.B.Bhushan.) (2011)
  • Author(s): Yu-ki Suzuki, Masatoshi Yokokawa, S.H.Yoshimura, Kunio Takeyasu
  • Publisher: Springer-Verlag, Heidelberg
• [Book] Scanning Probe Microscopy in Nanoscience and Nanotechnology, Vol 2 (2011)
  • Author(s): K.Takeyasu, 他3名
  • Publisher: Springer-Verlag, Heidelberg
• [Book] Microscopy : Science, Technology, Applications and Education(Force spectroscopy addresses fundamental problems in cell physiology.)(ed.A.Mendez-Vilas) (2010)
  • Author(s): Hirohide Takahashi, Yaron R.Silberberg, Masahiro Kumeta, J.Michael Edwardson, Kunio Takeyasu
  • Publisher: Formatex Research Center, Badajoz, Spain pp.
• [Book] Microscopy : Science, Technology, Applications and Education (2010)
  • Author(s): K.Takeyasu, 他4名
  • Publisher: Formatex Research Center, Badajoz, Spain
• [Book] Applied Scanning Probe Methods, Vol. 14 (2010)
  • Author(s): K.Takeyasu, 他4名
  • Publisher: Springer-Verlag, Heidelberg
• [Book] Applied Scanning Probe Methods, Vol.14(Modern Atomic Force Microscopy and Its Application to the Study of Genome Architecture.)(ed.B.Bhushan.) (2009)
  • Author(s): K.Takeyasu, H.Maruyama, Y.Suzuki, K.Hizume, S.H.Yoshimura
  • Publisher: Springer-Verlag, Heidelberg.
• [Book] Bacterial DNA Research Progress(Nucleoid Architecture and Dynamics in Bacteria.)(eds.Frank Columbus and Lauren Perl.) (2009)
  • Author(s): Ohniwa R.L., K.Morikawa, Ohta T., Wada C., Takeyasu K.
  • Publisher: NOVA Publishers.
• [Book] Applied Scanning Probe Methods(Modem Atomic Force Microscopy and Its Application to the Study of Genome Architecture., Vol. 14 ed) (2009)
  • Author(s): Takeyasu K, Maruyama H., Suzuki Y., Hizume K., Yoshimura S. H., B. Bhushan.
  • Publisher: Springer-Verlag
• [Book] Bacterial DNA Research Progress(eds Frank Columbus and Lauren Perl, Nucleoid Architecture and Dynamics in Bacteria.) (2009)
  • Author(s): Ohniwa R. L., K. Morikawa, Ohta T., Wada C., Takeyasu K.
  • Publisher: NOVA Publishers
• [Book] Chromatin and Disease(eds. T. K Kundu. D. Dipak , Structural organization of dynamic chromatin, Subsellular Biochemistry series, Vol. 41) (2009)
  • Author(s): Hizume K, Yoshimura S. H, Kumeta M, Takeyasu K.
  • Publisher: Springer Verlag
• [Book] Applied Scanning Probe Methods Vol.14 (2009)
  • Author(s): K.Takeyasu, 他4名
  • Publisher: Springer-Verlag, Heidelberg
• [Book] Bacterial DNA Research Progress (2009)
  • Author(s): K. Takeyasu
  • Publisher: Nova Science Publishers.(印刷中)
• [Book] )Chromatin and Disease(Structural organization of dynamic chromatin.)(eds.T.K.Kundu.D.Dipak )(Subsellular Biochemistry series Vol. 41) (2007)
  • Author(s): Hizume K, Yoshimura S.H, Kumeta M, Takeyasu K.
  • Publisher: SpringerVerlag.
• [Book] Biopolymer Research Trends(Biochemical and biophysical basis of genome folding mechanisms.)(ed.Pablo C.Sanchez.) (2007)
  • Author(s): Kobori T, Hizume K, Ohniwa R.L, Yoshimura S.H, Takeyasu K.
  • Publisher: Nova Science Publishers.
• [Book] Biopolymer Research Trends(ed. Pablo C. Sanchez, Biochemical and biophysical basis of genome folding mechanisms.) (2007)
  • Author(s): Kobori T, Hizume K, Ohniwa R. L, Yoshimura S. H, Takeyasu K.
  • Publisher: Nova Science Publishers
• [Book] Chromatin and Disease (2007)
  • Author(s): K. Hizume, S.H. Yoshimura, M. Kumeta, K. Takeyasu.
  • Total Pages: 433
  • Publisher: Springer Verlag.
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/labs.chrom.index.html