|Budget Amount *help
¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2009: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2008: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2007: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
More than 90% of mutations were GC to AT transition in the treatment of fertilized egg cells with MNU. The mutation frequency on the MNU treatment was 6 to 9 bases per 1kb, and thus if about three thousand mutant line is established, it will be expected that they cover at least four mutations for each of genes in rice genome, that is, they are the saturation mutant library. We established the effective screening methods using TILLING combined with the MNU mutant library and SDS-PAGE with modified extraction buffer, and some of key novel genes involving in starch biosynthesis and amyloplast engineering like as PHO1 and FLO2 were isolated and characterized by these methods. The profiling using the mutants induced by MNU treatment is effective to the systematic development of the novel genetic resources for starch and to the establishment of designed breeding method for novel starch properties in rice.