Molecular Evolutionary Studies on Sex Pheromone Communication System-Exploring Coevolution between Signal Emitters and Receivers-

• Project/Area Number: 19208005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: The University of Tokyo
• Principal Investigator: ISHIKAWA Yukio 東京大学, 大学院・農学生命科学研究科, 教授 (60125987)
• Co-Investigator(Kenkyū-buntansha): 田付 貞洋 東京大学, 農学部, 非常勤講師 (40163480)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000); Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2007: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
• Keywords: 性フェロモン / 生合成 / 嗅覚受容体 / 交信 / 進化 / アワノメイガ / Ostrinia / アズキノメイガ / フェロモンリセプター / 脂肪酸還元酵素 / アワノメイガ類 / 性フェロモン受容体 / クローニング / フェロモン受容体

Research Abstract

The sex pheromone communication systems in moths is highly developed. The sex pheromone production system in females and sex pheromone detection systems in males should have evolved in coordination in association with the speciation. We attempted to elucidate the mechanism that underlies the coordinated evolution by using moth species belonging to the genus Ostrinia. We cloned genes involved in the biosynthesis of sex pheromone components in females and genes encoding sex pheromone receptors in males.

Research Products

• [Journal Article] Sex-linked transcription factor involved in a shift of sex pheromone preference in the silkmoth, Bombyx mori (2011)
  • Author(s): Fujii T., T. Fujii, S. Namiki, H. Abe, T. Sakurai, A. Ohnuma, R. Kanzaki, S. Katsuma, Y. Ishikawa and T. Shimada
  • Journal Title: PNAS Volume: 108 Pages: 18038-18043
  • Peer Reviewed
• [Journal Article] Sex-linked pheromone receptor genes of the European corn borer, Ostrinia nubilalis, are in tandem arrays (2011)
  • Author(s): Yasukochi, Y., N. Miura, R. Nakano, K. Sahara and Y. Ishikawa
  • Journal Title: PLoS ONE Volume: 6(4)
  • Peer Reviewed
• [Journal Article] Sex pheromone desaturase functioning in a primitive Ostrinia moth is cryptically conserved in congeners' genomes (2011)
  • Author(s): Fujii, T., K. Ito, M. Tatematsu, T. Shimada, S. Katsuma and Y. Ishikawa
  • Journal Title: PNAS Volume: 108 Pages: 7102-7106
  • Peer Reviewed
• [Journal Article] Genetic basis regulating the sex pheromone blend in Ostrinia zealis(Lepidoptera : Crambidae) and its allies inferred from crossing experiments (2011)
  • Author(s): Tabata J. and Y. Ishikawa
  • Journal Title: Annal. Entomol. Soc. Amer Volume: 104 Pages: 326-336
  • Peer Reviewed
• [Journal Article] Isolation of BAC clones containing conserved genes from libraries of three distantly related moths : a useful resource for comparative genomics of Lepidoptera (2011)
  • Author(s): Yasukochi Y., M. Tanaka-Okuyama, M. Kamimura, R. Nakano, Y. Naito, Y. Ishikawa and K. Sahara
  • Journal Title: J. Biomed. Biotech Volume: 2011
  • NAID: 120003043350
  • Peer Reviewed
• [Journal Article] Cloning and functional characterization of a fatty acid transport protein(FATP) from the pheromone gland of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone (2011)
  • Author(s): Qian, S., T. Fujii, K. Ito, R. Nakano and Y. Ishikawa
  • Journal Title: Insect Biochem. Mol. Biol Volume: 41 Pages: 22-28
  • Peer Reviewed
• [Journal Article] Broadly and narrowly tuned odorant receptors are involved in female sex pheromone reception in Ostrinia moths (2010)
  • Author(s): Miura, N., T. Nakagawa, K. Touhara & Y. Ishikawa
  • Journal Title: Insect Biochem. Mol. Biol Volume: 40 Pages: 64-73
  • Peer Reviewed
• [Journal Article] Molecular and functional characterization of an acetyl-CoA acetyltransferase from the adzuki bean borer moth Ostrinia scapulalis(Lepidoptera : Crambidae) (2010)
  • Author(s): Fujii, T., K. Ito, S. Katsuma, R. Nakano, T. Shimada & Y. Ishikawa
  • Journal Title: Insect Biochem. Mol. Biol Volume: 40 Pages: 74-78
  • Peer Reviewed
• [Journal Article] Female sex pheromone of a lichen moth Eilema japonica(Arctiidae, Lithosiinae): components and control of production (2010)
  • Author(s): Fujii, T., R. Nakano, Y. Takubo, S. Qian, R. Yamakawa, T. Ando and Y. Ishikawa
  • Journal Title: J. Insect Physiol Volume: 56 Pages: 1986-1991
  • Peer Reviewed
• [Journal Article] Molecular and functional characterization of an acetyl-CoA acetyltransferase from the adzuki bean borer moth Ostrinia scapulalis (Lepidoptera : Crambidae) (2010)
  • Author(s): Fujii, T., K. Ito, S.Katsuma, R.Nakano, T.Shimada, Y.Ishikawa
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 40 Pages: 74-78
  • Peer Reviewed
• [Journal Article] Female sex pheromone of a lichen moth Eilema japonica (Arctiidae, Lithosiinae) : components and control of production (2010)
  • Author(s): Fujii, T., R.Nakano, Y.Takubo, S.Qian, R.Yamakawa, T.Ando, Y.Ishikawa
  • Journal Title: Journal of Insect Physiology Volume: 56 Pages: 1986-1991
  • Peer Reviewed
• [Journal Article] Broadly and narrowly tuned odorant receptors are involved in female sex pheromone reception in Ostrinia moths (2010)
  • Author(s): Miura, N., T. Nakagawa, K. Touhara, Y. Ishikawa
  • Journal Title: Insect Biochem.Mol.Biol. 40 Pages: 64-73
  • Peer Reviewed
• [Journal Article] Molecular and functional characterization of an acetyl-CoA acetyltransferase from the adzuki bean borer moth Ostrinia scapulalis (Lepidoptera : Crambidae) (2010)
  • Author(s): Fujii, T., K. Ito, S. Katsuma, R. Nakano, T. Shimada, Y. Ishikawa
  • Journal Title: Insect Biochem.Mol.Biol. 40 Pages: 74-78
  • Peer Reviewed
• [Journal Article] Broadly and narrowly tuned odorant receptors are involved in female sex pheromone reception in Ostrinia moths. (2010)
  • Author(s): Miura, N., T.Nakagawa, K.Toukara, Y.Ishikawa
  • Journal Title: Insect Biochemistry and Molecular Biology 40 Pages: 64-73
  • Peer Reviewed
• [Journal Article] A male-specific odorant receptor conserved through the evolution of sex pheromones in Ostrinia moth species (2009)
  • Author(s): Miura, N., T. Nakagawa, S. Tatsuki, K. Touhara & Y. Ishikawa
  • Journal Title: Int. J. Biol. Sci Volume: 5 Pages: 319-330
  • Peer Reviewed
• [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis(Lepidoptera : Crambidae) (2009)
  • Author(s): Antony, B., T. Fujii, K. Moto, S. Matsumoto, M. Fukuzawa, R. Nakano, S. Tatsuki & Y. Ishikawa
  • Journal Title: Insect Biochem. Mol. Biol Volume: 39 Pages: 90-95
  • Peer Reviewed
• [Journal Article] Alternative suppression of transcription from two desaturase genes is the key for species-specific sex pheromone biosynthesis in two Ostrinia moths (2009)
  • Author(s): Sakai, R., M. Fukuzawa, R. Nakano, S. Tatsuki & Y. Ishikawa
  • Journal Title: Insect Biochem. Mol. Biol Volume: 39 Pages: 62-67
  • Peer Reviewed
• [Journal Article] Alternative suppression of transcription from two desaturase genes is the key for species-specific sex pheromone biosynthesis in two Ostrinia moths (2009)
  • Author(s): Sakai, R., M. Fukuzawa, R. Nakano, S. Tatsuki, Y. Ishikawa
  • Journal Title: Insect Biochem.Mol.Biol. 39 Pages: 62-67
  • Peer Reviewed
• [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki bean borer, Ostrinia scapulalis (Lepidoptera : Crambidae) (2009)
  • Author(s): Antony, B., T. Fujii, K. Moto, S. Matsumoto, M. Fukuzawa, R. Nakano, S. Tatsuki, Y. Ishikawa
  • Journal Title: Insect Biochem.Mol.Biol. 39 Pages: 90-95
  • Peer Reviewed
• [Journal Article] A male-specific odorant receptor conserved through the evolution of sex pheromones in Ostrinia moth species (2009)
  • Author(s): Miura, N., T. Nakagawa, S. Tatsuki, K. Touhara, Y. Ishikawa
  • Journal Title: Int.J.Biol.Sci. 5 Pages: 319-330
  • Peer Reviewed
• [Journal Article] A male-specific odorant receptor conserved through the evolution of sex pheromones in Ostrinia moth species. (2009)
  • Author(s): Miura, N., T.Nakagawa, S.Tatsuki, K.Touhara, Y.Ishikawa
  • Journal Title: International Journal of Biological Sciences 5 Pages: 319-330
  • Peer Reviewed
• [Journal Article] A male-specific odorant receptor conserved through the evolution of sex pheromones in Ostrinia moth species (2009)
  • Author(s): Miura, N., T.Nakagawa, S.Tatsuki, K.Touhara, Y.Ishikawa
  • Journal Title: International Journal of Biological Sciences 5 Pages: 319-330
  • Peer Reviewed
• [Journal Article] Alternative suppression of transcription from two desaturase genesis the key for species-specific sex pheromone biosynthesis in two Ostrinia moths (2009)
  • Author(s): Sakai, R., M. Fukuzawa, R. Nakano, S. Tatsuki, Y. Ishikawa
  • Journal Title: Insect Biochemistry and Molecular Biology 39 Pages: 62-67
  • Peer Reviewed
• [Journal Article] Pheromone-gland-specific fatty-acyl reductase in the adzuki beanborer, Ostrinia scapulalis (Lepidoptera : Crambidae) (2009)
  • Author(s): Antony, B., T. Fujii, K. Moto, S. Matsumoto, M. Fukuzawa, R. Nakano, S. Tatsuki, Y. Ishikawa
  • Journal Title: Insect Biochemistry and Molecular Biology 39 Pages: 90-95
  • Peer Reviewed
• [Journal Article] Sex pheromone of Ostrinia sp. newly found on the leopard plant Farfugium japonicum (2008)
  • Author(s): Tabata, J., Y., Huang, S. Ohno, Y., Yoshiyasu, H. Sugie, S. Tatsuki & Y. Ishikawa
  • Journal Title: J. Appl. Entomol Volume: 132 Pages: 566-574
  • Peer Reviewed
• [Journal Article] Sex pheromone of Ostrinia sp. newly found on the leopard plant Farfugium japonicum (2008)
  • Author(s): Tabata, J., Y., Huang, S. Ohno, Y., Yoshiyasu, H. Sugie, S. Tatsuki, Y. Ishikawa
  • Journal Title: J.Appl.Entomol. 132 Pages: 566-574
  • Peer Reviewed
• [Presentation] ヨーロッパアワノメイガの触角でオス特異的に発現する嗅覚受容体遺伝子の重複 (2010)
  • Author(s): 安河内祐二・三浦奈美・石川幸男・佐原健
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学(長野県)
  • Year and Date: 2010-04-03
• [Presentation] アワノメイガ類における性フェロモン交信系の進化を探る-メスの性フェロモン生合成系とカスの性フェロモン受容系の研究から- (2010)
  • Author(s): 三浦奈美・中川龍郎・坂井亮太・福澤麻衣・東原和成・石川幸男
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2010-03-30
• [Presentation] ヨーロッパアワノメイガの触角でオス特異的に発現する嗅覚受容体遺伝子の重複 (2010)
  • Author(s): 安河内祐二・三浦奈美・石川幸男・佐原健
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学繊維学部
• [Presentation] アズキノメイガの性フェロモン腺で特異的に発現するfatty acyl reductase(FAR)遺伝子のクローニング (2009)
  • Author(s): 銭曙光・藤井毅・Binu Antony・石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] アズキノメイガOstrinia scapulalisのフェロモン腺で発現しているアセチル基転移酵素の解析 (2009)
  • Author(s): 藤井毅・伊藤克彦・勝間進・嶋田透・石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] 種特異的な性フェロモン生合成における不飽和化酵素遺伝子の調節機構 (2009)
  • Author(s): 坂井亮太・福澤麻衣・中野亮・田付貞洋・石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] アズキノメイガの性フェロモン腺で特異的に発現する fatty acyl reductase (FAR) 遺伝子のクローニング (2009)
  • Author(s): 銭曙光・藤井毅・Binu Antony・石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] アズキノメイガ Ostrinia scapulalis のフェロモン腺で発現しているアセチル基転移酵素の解析 (2009)
  • Author(s): 藤井毅・伊藤克彦・勝間進・嶋田透・石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] 種特異的な性フェロモン生合成における不飽和化酵素遺伝子の調節機構. 講演要旨C309 (2009)
  • Author(s): 坂井亮太・福澤麻衣・中野亮・田付貞洋・石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] アズキノメイガの性フェロモン腺で特異的に発現する fatty acyl reductase (FAR)遺伝子のクローニング (2009)
  • Author(s): 銭曙光, 藤井毅, Binu Antony, 石川幸男
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] 日本産アワノメイガ類の性フェロモン受容体遺伝子のクローニングと機能解析 (2007)
  • Author(s): 三浦奈美・中川龍郎・田付貞洋・東原和成・石川幸男
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13