Study on the prophylaxis effect of peptides on vascular-related diseases

• Project/Area Number: 19208012
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: MATSUI Toshiro Kyushu University, 農学研究院, 准教授 (20238942)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Kiyoshi 九州大学, 農学研究院, 教授 (80038322) ; SATO Masao 九州大学, 農学研究院, 准教授 (90294909) ; KIMOTO Koichi 東京家政大学, 家政学部, 教授 (60133378)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2007: ¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000)
• Keywords: ペプチド / 高血圧 / 動脈硬化 / LC-MS / MS / 血管機能 / 生活習慣病 / MD

Research Abstract

The object of this study was to clarify the prophylaxis effect of small peptides on vascular-related diseases. As a result, peptides having basic amino acids could evoke a significant vasorelaxation effect in contracted rat aorta. The effect was closely associated with the reduction of intracellular Ca2+ through a Ca2+ channel. In addition, a 9-wk administration of WH to apo E deficient mice successfully improved a significant atherosclerosis area.

Research Products

• [Journal Article] Vasodilating di-peptide Trp-His can prevent atherosclerosis in apolipoprotein E-deficient mice. (2010)
  • Author(s): Matsui T, Sato M, Tanaka M, Yamada Y, Watanabe S, Fujimoto Y, Imaizumi K, Matsumoto K
  • Journal Title: Br. J. Nutr. 103(3) Pages: 309-313
• [Journal Article] Vasodilating di-peptide Trp-His can prevent atherosclerosis in apolipopr otein E-deficient mice (2010)
  • Author(s): T.Matsui, et al
  • Journal Title: Br. J. Nutr.. 103 Pages: 309-313
  • Peer Reviewed
• [Journal Article] α-Glucosidase inhibition assay in an enzyme-immobilized amino-microplate. (2009)
  • Author(s): Matsui T, Shimada M, Saitoh N, Matsumoto K
  • Journal Title: Anal. Sci. 25(4) Pages: 559-562
  • NAID: 10025211881
• [Journal Article] His-Arg-Trp potently attenuates contracted tension of thoracic aorta of Sprague-Dawley rats through the suppression of extracellular Ca2+ influx. (2009)
  • Author(s): Tanaka M, Watanabe S, Wang Z, Matsumoto K, Matsui T
  • Journal Title: Peptides 30 Pages: 1502-1507
• [Journal Article] His-Arg-Trp potently attenuates contracted tension of thoracic aorta of Sprague-Dawley rats through the suppression of extracellular Ca^<2+>influx (2009)
  • Author(s): T.Matsui, et al
  • Journal Title: Peptides 30 Pages: 1502-1507
  • Peer Reviewed
• [Journal Article] Identification of ACE-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers. (2008)
  • Author(s): Zhu XL, Matsui T, Watanabe K, Shiraishi K, Ueki T, Matsumoto K
  • Journal Title: Peptides 29(3) Pages: 338-344
• [Journal Article] Endothelium-independent vasodilation effect of di-and tri-peptides in thoracic aorta of Sprague-Dawley rats. (2008)
  • Author(s): Tanaka M, Tokuyasu M, Matsui T, Matsumoto K
  • Journal Title: Life Sci 82(15-16) Pages: 869-875
• [Journal Article] Structure-activity relationship between vasodilating di-and tri-peptides in thoracic aorta of Sprague-Dawley rats. (2008)
  • Author(s): Tanaka M, Watanabe S, Tokuyasu M, Matsui T, Matsumoto K
  • Journal Title: J. Hypertension 26(suppl.1)
• [Journal Article] Endothelium-independent vasodilation effect of di- and tri-peptides in thoracic aorta of Sprague-Dawley rats. (2008)
  • Author(s): T. Matsui, et al
  • Journal Title: Life Sciences 82 Pages: 869-875
  • Peer Reviewed
• [Journal Article] Structure-activity relationship between vasodilating di- and tri-peptides in thoracic aorta of Sprague-Dawley rats (2008)
  • Author(s): T, Matsui, et al
  • Journal Title: J. Hypertension 26
  • Peer Reviewed
• [Journal Article] Enndothelium-independent vasodilation effect of di-and tri-peptides in thoracic aorta of Sprague-Dawley rats (2008)
  • Author(s): T.Matsui, et. al.
  • Journal Title: Life Sciences In accept(掲載決定)
  • Peer Reviewed
• [Journal Article] Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique. (2007)
  • Author(s): Zhu XL, Matsui T, Watanabe K, Shiraishi K, Ueki T, Matsumoto K
  • Journal Title: J. Agric. Food Chem. 55(21) Pages: 8367-8374
• [Journal Article] Slimultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique (2007)
  • Author(s): X-L.Zhu, T.Matsui, et. al.
  • Journal Title: Journal of Agricultural and Food Chemistry 55 Pages: 8367-8374
  • Peer Reviewed
• [Presentation] 生理活性ペプチド評価法としての分子動力学(MD)シミュレーション法の構築 (2009)
  • Author(s): 中島健太郎、松井利郎, ら
  • Organizer: H21年度日本栄養・食糧学会九州・沖縄支部大会
  • Place of Presentation: 琉球大学農学部
  • Year and Date: 2009-10-31
• [Presentation] 血管弛緩性ジペプチドTrp-Hisの抗動脈硬化作用とその発現機構解明 (2009)
  • Author(s): 田中充、松井利郎, ら
  • Organizer: H21年度日本栄養・食糧学会九州・沖縄支部大会
  • Place of Presentation: 琉球大学農学部
  • Year and Date: 2009-10-31
• [Presentation] Structure-activity relationship of vasodilating small peptides in rat aortic rings. (2009)
  • Author(s): S. Watanabe, T. Matsui, et al
  • Organizer: 14th IUFoST
  • Place of Presentation: Shanghai (China)
  • Year and Date: 2009-10-18
• [Presentation] Antihypertensive mechanism of small peptides. (2009)
  • Author(s): T. Matsui
  • Organizer: 99th AOCS
  • Place of Presentation: eattle (USA)
  • Year and Date: 2009-05-21
• [Presentation] トリペプチドにおける血管弛緩作用-構造相関の検討. (2009)
  • Author(s): 渡辺慎平、松井利郎, ら
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: ブリックホール(長崎市)
  • Year and Date: 2009-05-21
• [Presentation] トリペプチドにおける血管弛緩作用-構造相関の検討 (2009)
  • Author(s): 渡辺慎平、松井利郎, ら
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: ブリックホール(長崎市)
  • Year and Date: 2009-05-21
• [Presentation] Caco-2細胞を用いたジペプチド類の吸収挙動とその理論的把握 (2009)
  • Author(s): 吉浦慶子、松井利郎, ら
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: ブリックホール(長崎市)
  • Year and Date: 2009-05-21
• [Presentation] Structure-activity relationship of vasodilating small peptides in rat aortic rings (2008)
  • Author(s): S. Watanabe, T. Matsui, et al
  • Organizer: 14^<th> IUFoST
  • Place of Presentation: Shanghai(China)
  • Year and Date: 2008-10-18
• [Presentation] Antihypertensive mechanism of small peptides (2008)
  • Author(s): T. Matsui
  • Organizer: 99^<th> AOCS
  • Place of Presentation: Seattle(USA)
  • Year and Date: 2008-05-21
• [Presentation] 血管弛緩性低分子ペプチドの構造―活性相関の解明及びその作用機序の解明。 (2008)
  • Author(s): 渡辺慎平、松井利郎, ら
  • Organizer: 第62回日本栄養・食料学会大会.
  • Place of Presentation: 女子栄養大学(埼玉県)
  • Year and Date: 2008-05-02
• [Presentation] 血管弛緩性低分子ペプチドの構造-活性相関の解明及び園作用機序の解明 (2008)
  • Author(s): 渡辺慎平, 松井利郎ら
  • Organizer: 第62回日本栄養・食料学会大会
  • Place of Presentation: 女子栄養大学(埼玉県)
  • Year and Date: 2008-05-02
• [Presentation] Vasodilating small peptides and their machanism in isolated rat thoracic aortas. (2007)
  • Author(s): M. Tanaka, T. Matsui, et al
  • Organizer: Symposium in multi-functionality and sustainability of land use in south-Asia and Eastern-Asia.
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2007-09-12
• [Presentation] Vasodilating small peptides and their machanism in isolated rat thoracic aortas (2007)
  • Author(s): M.Tanaka, T.Matsui, et. al.
  • Organizer: Symposium in multi-functionality and sustainability of land use in south-Asia and Eastern-Asia
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2007-09-12
• [Presentation] 血管弛緩作用を有する低分子ペプチドの検索と作用機構の解明. (2007)
  • Author(s): 徳安茉依、松井利郎, ら
  • Organizer: 第54回日本食品科学工学会大会.
  • Place of Presentation: 村学園大学(福岡市)
  • Year and Date: 2007-09-07
• [Presentation] 血管弛緩作用を有する低分子ペプチドの検索と作用機構の解明 (2007)
  • Author(s): 徳安 茉依、松井 利郎, ら
  • Organizer: 第54回日本食品科学工学会大会
  • Place of Presentation: 中村学園大学(福岡市)
  • Year and Date: 2007-09-07
• [Book] 第13章タンパク質とペプチド・アミノ酸の機能性 (2008)
  • Author(s): 松井利郎
  • Publisher: “食品機能性の科学"西川研次郎監修(産業技術サービスセンター)東京
• [Book] Anti-hypertensive peptides and their underlying mechanisms (chapter 2) In Biologically Active Food Proteins and Peptides in Health-Fundamental and Clinical aspects-(Eds.) Mine
  • Author(s): Toshiro Matsui, Mitsuru Tanaka, Y., Li-Chan
  • Publisher: IFT Book Series, Wiley Blackwell (Iowa)(in press)
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/biosci-biotech/bunseki/
• [Patent(Industrial Property Rights)] 抗アテローム性動脈硬化作用のあるジペプチド (2009)
  • Inventor(s): 松井利郎
  • Industrial Property Rights Holder: 九州大学。不二製油(株)
  • Industrial Property Number: 2009-060978
  • Filing Date: 2009-03-31
  • Acquisition Date: 2009-03-31