Regeneration of intestinal mucosa and molecular mechanism of the failure of intestinal epithelial cells under intestinal inflammation.

• Project/Area Number: 19209027
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: WATANABE Mamoru Tokyo Medical and Dental University, 医歯学総合研究科, 教授 (10175127)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Kiyoshi 大阪大学, 医学系研究科, 教授 (20309446) ; HANDA Hiroshi 東京工業大学, 生命理工学研究科, 教授 (80107432) ; 土屋 輝一郎 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40376786) ; 永石 宇治 東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座教員 (60447464) ; 深尾 太郎 東京医科歯科大学, 大学院・医歯学総合研究科, メティカルフェロー (20401127)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000); Fiscal Year 2009: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2008: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2007: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
• Keywords: 腸管再生 / Wntシグナル / Notchシグナル / Atoh1 / 炎症性腸疾患 / Wnt / Notch

Research Abstract

Significant results were provided as we showed it in this study as follows.
1) We showed that the up-regulation of Notch signal in inflammatory bowel disease was necessary to promote cell proliferation to maintain mucosal barrier with a sacrifice of differentiation state.
2) We identified Hes1 as the critical gene to suppress the differentiation of intestinal mucosa under the Notch signal stimulation in inflammatory bowel disease, using microarray examination.
3) We showed that Hes1 protein is highly expressed on the chronic inflammation mucosa under the Notch signal up-regulation in inflammatory bowel disease.
4) Hes1 suppress goblet cell differentiation by the repression of Hath1 gene transcription.
5) Hes1 directly binds to the 5' promoter region of Hath1 resulting in the suppression of Hath1 gene expression, using ChIP assay.

Research Products

• [Journal Article] Magneto-optical biosensing platform based on light scattering from self-assembled chains of functionalized rotating magnetic beads (2010)
  • Author(s): Park S.Y, Handa H, Sandhu A
  • Journal Title: Nano Lett. 10 Pages: 446-451
  • Peer Reviewed
• [Journal Article] Identification of dynamin-2-mediated endocytosis as a new target of osteoporosis drugs, bisphosphonates (2010)
  • Author(s): Masaike Y, Takagi T, Hirota M, Yamada J, Ishihara S, Yung T M C, Inoue T, Sawa C, Sagara H, Sakamoto S, Kabe Y, Takahashi Y, Yamaguchi Y, Handa H
  • Journal Title: Mol.Pharmacol. 77 Pages: 262-269
  • Peer Reviewed
• [Journal Article] Delta-like 1 expression promotes goblet cell differentiation in Notch-inactivated human colonic epithelial cells. (2010)
  • Author(s): Akiyama J, Watanabe M, et al.
  • Journal Title: Biochemical and Biophysical Research Communications. (in press)
  • Peer Reviewed
• [Journal Article] Requirement of Notch activation during regeneration of the intestinal epithelia (2009)
  • Author(s): Okamoto R, Tsuchiya K, Nemoto Y, Akiyama J, Nakamura T, Kanai T, Watanabe M
  • Journal Title: Am J Physiol GI & Liver 296
  • Peer Reviewed
• [Journal Article] RANK-RANKL signaling pathway is critically involved in the function of CD4+CD25+ regulatory T Cells in chronic colitis (2009)
  • Author(s): Totsuka T, Kanai T, Nemoto Y, Tomita T, Okamoto R, Tsuchiya K, Nakamura T, Sakamoto N, Akiba H, Okumura K, Yagita H, Watanabe M
  • Journal Title: J Immunol. 182 Pages: 6079-6087
  • Peer Reviewed
• [Journal Article] Long-Lived colitogenic CD4+ Memory T Cells residing outside the intestine participate in the perpetuation of chronic colitis (2009)
  • Author(s): Nemoto Y, Kanai T, Kameyama K, Shinohara T, Sakamoto N, Totsuka T, Okamoto R, Tsuchiya K, Nakamura T, Sudo T, Matsumoto S, Watanabe M
  • Journal Title: J Immunol. 183 Pages: 5059-5068
  • Peer Reviewed
• [Journal Article] Induction of Intestinal Th17 cells bysegmented filamentous bacteria (2009)
  • Author(s): Ivanov II, Atarashi K, Manel N, Brodie EL,Shima T, Karaoz U, Wei D, Goldfarb KC, Santee CA, Lynch S V, Tanoue T, Imaoka A, Itoh K, Takeda K, Umesaki Y, Honda K, Littman DR
  • Journal Title: Cell 139 Pages: 485-498
  • Peer Reviewed
• [Journal Article] NFATc1 mediates Toll-like receptor-independent innate immune responses during Trypanosoma cruzi infection (2009)
  • Author(s): Kayama H, Koga R, Atarashi K, Mak T W, Takayanagi H, Honda K, Yamamoto M., Takeda K
  • Journal Title: PLoS Pathogens 5
  • Peer Reviewed
• [Journal Article] Regulatory mechanisms of immune responses to intestinal bacteria (2009)
  • Author(s): Honda K, Takeda K
  • Journal Title: Mucosal Immunol 2 Pages: 187-196
  • Peer Reviewed
• [Journal Article] Repression of RNA Polymerase II elongation in vivo is critically dependent on the C-terminus of Spt-5 (2009)
  • Author(s): Chen H, Contreras X, Yamaguchi Y, Handa H, Peterlin B. M, Guo S
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Novel applications of affinity beads (2009)
  • Author(s): Kabe Y, Hatakeyama M, Sakamoto S, Nishio K, Handa H
  • Journal Title: In Protein Targeting with Small Molecules : Chemical Biology Techniques and Applications(ed. Oasda, H)(John Wiley & Sons, Inc, New Jersey) Pages: 39-56
  • Peer Reviewed
• [Journal Article] Preparation of spherical and uniform-sized ferrite nanoparticles with diameters between 50-150 nm for biomedical applications (2009)
  • Author(s): Tanaka T, Shimazu R, Nagai H, Tada M, Nakagawa T, Sandhu A, Handa H, Abe M
  • Journal Title: J.Magn.Magn.Mater. 321(10) Pages: 1417-1420
  • Peer Reviewed
• [Journal Article] Preparation of conophylline affinity nano-beads and identification of target preotein (2009)
  • Author(s): Suzuki E, Ogura H, Kato K, Takei I, Kabe, Y, Handa H, Umezawa K
  • Journal Title: Bioorg.Med.Chem. 17 Pages: 6188-6195
  • Peer Reviewed
• [Journal Article] Incorporation of 8-hydroxyguanosine 5'-triphosphate (8-oxo-7,8-dihydroguanosine5'-triphospha te) by bacterial and human RNA polymerases (2009)
  • Author(s): Kamiya H, Suzuki A, Yamaguchi Y, Handa H, Harashima H
  • Journal Title: Free Radic.Biol.Med. 46 Pages: 1703-1707
  • Peer Reviewed
• [Journal Article] Preparation and medical application of magnetic beads conjugated with bioactive molecules (2009)
  • Author(s): Abe M, Nishio K, Hatakeyama M, Hanyu N, Tanaka T, Tada M, Nakagawa T, Sandhu A, Handa H
  • Journal Title: J.Magn.Magn.Mater. 321 Pages: 645-649
  • Peer Reviewed
• [Journal Article] High-throughput bioscreening system utilizing high-performance affinity magnetic carriers exhibiting minimal non-specific protein binding (2009)
  • Author(s): Hanyu N, Nishio K, Hatakeyama M, Yasuno H, Tanaka T, Tada M, Nakagawa T, Sandhu A, Abe M, Handa H
  • Journal Title: J.Magn.Magn.Mater. 321 Pages: 1625-1627
  • Peer Reviewed
• [Journal Article] Synthesis of hollow ferrite nanospheres for biomedical applications (2009)
  • Author(s): Tada M, Kanemaru T, Hara T, Nakagawa T, Handa H, Abe M
  • Journal Title: J.Magn.Magn.Mater. 321 Pages: 1414-1416
  • Peer Reviewed
• [Journal Article] The Ape-1/Ref-1 redox antagonist E3330 inhibits the growth of tumor endothelium and endothelial progenitorcells : Therapeutic implications in tumor angiogenesis (2009)
  • Author(s): Zou G-M, Karikari C, Kabe Y, Handa H, Anders R. A, Maitra A
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  • Peer Reviewed
• [Journal Article] High speed magneto-optical valve : Rapid control of the optical transmittance of aqueous solutions by magnetically induced self-assembly of superparamagnetic particle chains (2009)
  • Author(s): Park S Y, Handa H, Sandhu A
  • Journal Title: J.Appl.Phys. 105
  • Peer Reviewed
• [Journal Article] High sensitivity and multifunctional micro-Hall sensors fabricated using InAlSb/InAsSb/InAlSb heterostructures (2009)
  • Author(s): Bando M, Ohashi T, Dede M, Akram R, Oral A, Park S Y, Shibasaki I, Handa H, Sandhu A
  • Journal Title: J.Appl.Phys 105
  • Peer Reviewed
• [Journal Article] Detection of Magnetic Nanobeads by Self-Assembly of Superparamagnetic Microbeads for Biosensing (2009)
  • Author(s): Morimoto Y, Abe M, Hatakeyama M, Handa H, Sandhu A
  • Journal Title: IEEE Transactions on Magnetics 45 Pages: 2871-2874
  • Peer Reviewed
• [Journal Article] Development and application of high-performance affinity beads : toward chemical biology and drug discovery (2009)
  • Author(s): Sakamoto S, Kabe Y, Hatakeyama M, Yamaguchi Y, Handa H
  • Journal Title: Chem.Rec. 9 Pages: 66-85
  • Peer Reviewed
• [Journal Article] Capsaicin binds to prohibitin 2 and displacesit from the mitochondria to the nuclus (2009)
  • Author(s): Kuramori C, Azuma M, Kume K, Kaneko Y, Inoue A, Yamaguchi Y, Kabe Y, Hosoya T, Kizak M, Suematsu M, Handa H
  • Journal Title: Biochem.Biophys.Res.Commun. 379 Pages: 519-525
  • Peer Reviewed
• [Journal Article] Characteization of a magnetic carrier encapsulating europium and ferrite nanoparticles for biomolecular recognition and imaging (2009)
  • Author(s): Hatakeyama M, Mochizuki Y, Kita H, Kishi K, Nishio S, Sakamoto M, Abe, M., Handa, H.
  • Journal Title: J.Magn.Magn.Mater. 321 Pages: 1364-1367
  • Peer Reviewed
• [Journal Article] The role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis (2009)
  • Author(s): Komori K, Inukai N, Yamada T, Yamaguchi Y, Handa H
  • Journal Title: Genes Cells. 14 Pages: 343-354
  • Peer Reviewed
• [Journal Article] 17 β-hydroxysteeroid dehydrogenase type 12 in human breast carcinoma : a prognostic factor via potential regulation of fatty acids synthesis (2009)
  • Author(s): Nagasaki S, Suzuki T, Miki Y, Akahira J-I, Kitada K, Ishida T, Handa H, Ohuchi N, Sasano H
  • Journal Title: Cancer Res. 69 Pages: 1392-1399
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• [Journal Article] A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress (2009)
  • Author(s): Kang J, Gemberling M, Nakamura M, Whitby F G, Handa H, Fairbrother W, Tantin D
  • Journal Title: Genes Dev. 23 Pages: 208-222
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• [Journal Article] Signaling pathway via TNFα/NFκB in intestinal epithelial cells may be directly involved in colitis-associated carcinogenesis. (2009)
  • Author(s): Onizawa M, Watanabe M, et al.
  • Journal Title: Am J Physiol GI & Liver. 296
  • Peer Reviewed
• [Journal Article] Requirement of Notch activation during regeneration of the intestinal epithelia. (2009)
  • Author(s): Okamoto R, Watanabe M, et al.
  • Journal Title: Am J Physiol GI & Liver. 296
  • Peer Reviewed
• [Journal Article] Long-Lived colitogenic CD4+ Memory T Cells residing outside the intestine participate in the perpetuation of chronic colitis. (2009)
  • Author(s): Nemoto Y, Watanabe M, et al.
  • Journal Title: J Immunol. 183 Pages: 5059-5068
  • Peer Reviewed
• [Journal Article] Musashi-1 suppresses expression of Paneth cell specific genes in human intestinal epithelial cells. (2009)
  • Author(s): Murayama M, Watanabe M, et al.
  • Journal Title: J Gastroenterol. 44 Pages: 173-182
  • NAID: 10025173646
  • Peer Reviewed
• [Journal Article] RANK-RANKL signaling pathway is critically involved in the function of CD4+CD25+ regulatory T Cells in chronic colitis. (2009)
  • Author(s): Totsuka T, Watanabe M, et al.
  • Journal Title: J Immunol. 182 Pages: 6079-3087
  • Peer Reviewed
• [Journal Article] IL-7 is essential for lymphopenia-driven turnover of colitogenic CD4+ memory T cells in chronic colitis. (2009)
  • Author(s): Tomita T, Watanabe M, et al.
  • Journal Title: Eur J Immunol. 39 Pages: 2737-2747
  • Peer Reviewed
• [Journal Article] Signaling pathway via TNFα/NFκB in intestinal epithelial cells may be directly involved in colitis-associated carcinogenesis. (2009)
  • Author(s): Onizawa M, Watanabe M, et al.
  • Journal Title: Am J Physiol GI & Liver. (in press)
  • Peer Reviewed
• [Journal Article] Negative feedback regulation of colitogenic CD4+ T cells by increased granulopolesis. (2009)
  • Author(s): Nemoto Y, Watanabe M, et al.
  • Journal Title: Inflamm Bowel Dis. 14 Pages: 1491-1503
  • Peer Reviewed
• [Journal Article] MyD88-dependent pathway in T cells directly modulates the expansion and survival of colitogenic CD4+ T cells in chronic colitis (2008)
  • Author(s): Tomita T, Kanai T, Fujii T, Nemoto Y, Okamoto R, Tsuchiya K, Totsuka T, Sakamoto N, Akira S, Watanabe M
  • Journal Title: J Immunol. 180 Pages: 5291-5299
  • Peer Reviewed
• [Journal Article] Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis (2008)
  • Author(s): Tomita T, Kanai T, Nemoto Y, Totsuka T, Okamoto R, Tsuchiya K, Sakamoto N, Watanabe M
  • Journal Title: J Immunol. 180 Pages: 383-390
  • Peer Reviewed
• [Journal Article] ATP drives lamina propria TH17 cell differentiation (2008)
  • Author(s): Atarashi K, Nishimura J, Shima T, Umesaki Y, Yamamoto M, Onoue M, Yagita H, Ishii N, Evans R, Honda K, Takeda K
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• [Journal Article] Role of nuclear IkB proteins in the regulation of host immune responses (2008)
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• [Journal Article] Class-specific regulation of pro-inflammatory genes by MyD88 pathways and IkBz (2008)
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• [Journal Article] SV40 vectors carrying minimal sequence of viral origin with exchangeable capsids (2008)
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  • Journal Title: Virology 379 Pages: 110-117
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• [Journal Article] Presentation of functional foreign peptides on the surface of SV40 virus-like particles (2008)
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• [Journal Article] Adenine nucleotide translocator transports haem precursors into mitochondria (2008)
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• [Journal Article] Tissu-and context-dependent modulation of hormonal sensitivity of glucocorticoid-responsive genes by HEXIM1 (2008)
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• [Journal Article] A new APE1/Ref-1-dependent pathway leading to reduction of NF-kB and AP-1, and activation of their DNA-binding activity (2008)
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  • Journal Title: Nucleic Acid Res. 36 Pages: 4327-4336
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• [Journal Article] Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm (2008)
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• [Journal Article] Development of a chemical screening system using aqueorin-fused protein (2008)
  • Author(s): Suzuki M, Furukawa S, Kuramori C, Sawa, C, Kabe Y, Nakamura M, Sawada J, Yamaguchi Y, Sakamoto S, Inoue, S, Handa H
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• [Journal Article] Development of novel magnetic nano-carriers for high-performance affinity purification (2008)
  • Author(s): Nishio K, Masaike Y, Ikeda M, Narimatsu H, Gokon N, Tsubouchi S, Hatakeyama M, Sakamoto S, Hanyu N, Sandhu A, Kawaguchi H, Abe M, Handa H
  • Journal Title: Colloid and Surface B : Biointerfaces. 64 Pages: 162-169
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• [Journal Article] A new mechanism of 6-((2-(dimethylamino)ethyl)amino)-3-hydroxy-7H-indeno(2,1-c)quinolin-7-one dihydrochloride (TAS-103) action discovered by target screening with drug-immobilized affinity beads (2008)
  • Author(s): Yoshida M, Kabe Y, Wada T, Asai A, Handa H
  • Journal Title: Mol.Pharmacol. 73 Pages: 987-994
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• [Journal Article] Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with minor capsid proteins VP2/3 (2008)
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• [Journal Article] Isolation of XAB2 complex involved in pre-mRNA splicing, transcription and transcription-coupled repair (2008)
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• [Journal Article] FTY720 suppresses the development of colitis in lymphoid-null mice by modulating the trafficking of colitogenic CD4+ T cells in bone marrow. (2008)
  • Author(s): Fujii F, Watanabe M, et al.
  • Journal Title: Eur J Immunol. 38 Pages: 3290-3303
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• [Journal Article] Colitogenic CD4^+ effector-memory T cells actively recirculate in chronic colitic mice. (2008)
  • Author(s): Tomita T, Watanabe M, et al.
  • Journal Title: Inflamm Bowel Dis. 14 Pages: 1630-1640
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• [Journal Article] Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells. (2008)
  • Author(s): Yoshioka A, Watanabe M, et al.
  • Journal Title: Cytokine. 44 Pages: 57-64
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• [Journal Article] Continuous generation of colitogenic CD4+ T cells in persistent colitis. (2008)
  • Author(s): Tomita T, Watanabe M, et al.
  • Journal Title: Eur J Immunol. 38 Pages: 1264-1274
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• [Journal Article] Inducible expression of microRNA-194 is regulated by HNF-1 during intestinal epithelial cell differentiation. (2008)
  • Author(s): Hino K, Watanabe M, et al.
  • Journal Title: RNA. 14 Pages: 1433-1442
  • Peer Reviewed
• [Journal Article] MyD88-dependent pathway in T cells directly modulates the expansion and survival of colitogenic CD4+ T cells in chronic colitis. (2008)
  • Author(s): Tomita T, Watanabe M, et al.
  • Journal Title: J Immunol. 180 Pages: 5291-5299
  • Peer Reviewed
• [Journal Article] Immunosenescent colitogenic CD4+ T cells convert to regulatory cells to suppress colitis. (2008)
  • Author(s): Totsuka T, Watanabe M, et al.
  • Journal Title: Eur J Immunol. 38 Pages: 1275-1286
  • Peer Reviewed
• [Journal Article] Proteasomal degradation of Atoh1 by aberrant Wnt signaling maintains the undifferentiated state of colon cancer. (2008)
  • Author(s): Aragaki M, Watanabe M, et al.
  • Journal Title: Biochem Biophys Res Commun. 368 Pages: 923-929
  • Peer Reviewed
• [Journal Article] Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis. (2008)
  • Author(s): Tomita T, Watanabe M, et al.
  • Journal Title: J Immunol. 180 Pages: 383-390
  • Peer Reviewed
• [Journal Article] Blockade of NKG2D signaling prevents the development of murine CD4+ T cell-mediated colitis. (2008)
  • Author(s): Ito Y, Watanabe M, et al.
  • Journal Title: Am J Physiol GI & Liver. 394
  • Peer Reviewed
• [Journal Article] Immunosenescent colitogenic CD4+ T cells convert to regulatory cells and suppress colitis. (2008)
  • Author(s): Totsuka T, Watanabe M, et. al.
  • Journal Title: Eur J Immunol. (in press)
  • Peer Reviewed
• [Journal Article] MyD88-dependent pathway in T Cells directly modulates the expansion of colitogenic CD4+ T Cells in chronic colitisl. (2008)
  • Author(s): Tomita T, Watanabe M, et. al.
  • Journal Title: J Immunol. (in press)
  • Peer Reviewed
• [Journal Article] Proteasomal degradation of Atohl by aberrant Wnt signaling maintains the undifferentiated state of colon cancer. (2008)
  • Author(s): Aragaki M, Watanabe M, et. al.
  • Journal Title: Biochem Biophys Res Commun. 368 Pages: 923-929
  • Peer Reviewed
• [Journal Article] Systemic, but not intestinal, IL-7 is essential for the persistence of chroniccolitis. (2008)
  • Author(s): Tomita T, Watanabe M, et. al.
  • Journal Title: J Immunol. 180 Pages: 383-390
  • Peer Reviewed
• [Journal Article] Bone marrow retaining colitogenic CD4+ T cells may be a pathogenic reservoir for chronic colitis (2007)
  • Author(s): Nemoto Y, Kanai T, Makita S, Okamoto R, Totsuka T, Takeda K, Watanabe M
  • Journal Title: Gastroenterology 132 Pages: 176-189
  • Peer Reviewed
• [Journal Article] IL-7 is essential for the development and the persistence of chronic colitis (2007)
  • Author(s): Totsuka T, Kanai T, Nemoto Y, Makita S, Okamoto R, Tsuchiya K, Watanabe M
  • Journal Title: J Immunol. 178 Pages: 4737-4748
  • Peer Reviewed
• [Journal Article] Intestinal lamina propria retaining CD4+CD25+ regulatory T cells is a suppressive site of intestinal inflammation (2007)
  • Author(s): Makita S, Kanai T, Nemoto Y, Totsuka T, Okamoto R, Tsuchiya K, Yamamoto M, Kiyono H, Watanabe M
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• [Journal Article] The Enteropathogenic E. coli effector EspB facilitates microvillus effacing and antiphagocytosis by inhibiting myosin function (2007)
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• [Journal Article] Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles (2007)
  • Author(s): Tsukamoto H, Kawano M, Inoue T, Enomoto T, Takahashi R, Yokoyama N, Yamamoto N, Imai T, Kataoka K, Yamaguchi Y, Handa H
  • Journal Title: Genes Cells. 12 Pages: 1267-1279
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• [Journal Article] Hepatitis delta antigen binds to the clamp of RNA polymerase II and affects transcriptional fidelity (2007)
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• [Journal Article] DSIF contributes to transcriptional activation by DNA-binding activators by preventing pausing during transcription elongation (2007)
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• [Journal Article] Differential regulation of NF-kappaB by elongation factors is determined by core promoter type (2007)
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• [Journal Article] RNA polymerase II bypasses 8-oxoguanine in the presence of transcription elongation factor TFIIS (2007)
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  • Journal Title: DNA Repair. 6 Pages: 841-851
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• [Journal Article] NELF interacts with CBC and participates in 3'-end processing of replication-dependent histone mRNAs (2007)
  • Author(s): Narita T, Yung T M C, Yamamoto J Tsuboi Y, Tanabe H, Tanaka K, Yamaguchi Y, Handa H
  • Journal Title: Mol Cell. 26 Pages: 349-365
  • Peer Reviewed
• [Journal Article] High efficiency Hall effect micro-biosensor platform for detection of magnetically labeled biomolecules (2007)
  • Author(s): Sandhu A, Kumagai Y, Lapicki A,Sakamoto S, Abe A, Handa H
  • Journal Title: Biosensors and Bioelectronics 22 Pages: 2115-2120
  • Peer Reviewed
• [Journal Article] Mutational analysis of the carboxyl-terminal region of the SV40 major capsid protein VP1 (2007)
  • Author(s): Yokoyama N, Kawano M, Tsukamoto H, Enomoto T, Inoue T, Takahashi R, Nakanishi A, Imai T, Wada T, Handa H
  • Journal Title: J.Biochem. 141 Pages: 279-286
  • NAID: 10020101612
  • Peer Reviewed
• [Journal Article] Minor capsid proteins of SV40 are dispensable for nucleocapsid assembly and cell entry, but are required for nuclear entry of the viral genome (2007)
  • Author(s): Nakanishi A, Itoh N, Li P P, Handa H, Liddington R C, Kasamatsu H
  • Journal Title: J.Virol. 81 Pages: 3778-3785
  • Peer Reviewed
• [Journal Article] Preparation of size controlled (30-100 nm) magnetite nanoparticles for biomedical applications (2007)
  • Author(s): Nishio K, Ikeda M, Gokon N, Tsubouchi S, Narimatsu H, Mochizuki Y, Sakamoto S, Snadhu A, Abe M, Handa H
  • Journal Title: J.Magn.Magn.Mater. 310 Pages: 2408-2410
  • Peer Reviewed
• [Journal Article] Identification of a chemical substance that is immobilized to ferrite nanoparticles (FP) (2007)
  • Author(s): Nishio K, Gokon N, Hasegawa M, Ogura Y, Ikeda M, Narimatsu H, Tada M, Yamaguchi Y, Sakamoto S, Abe M, Handa H
  • Journal Title: Colloid and Surface B : Biointerfaces 54 Pages: 249-253
  • Peer Reviewed
• [Journal Article] Adeno-associated virus site-specific integration is regulated by TRP-185 (2007)
  • Author(s): Yamamoto N, Suzuki M, Kawano M, Inoue T, Takahashi R, Tsukamoto H, Enomoto T, Yamaguchi Y, Wada T, Handa H
  • Journal Title: J.Virol. 81 Pages: 1990-2001
  • Peer Reviewed
• [Journal Article] hnRNP-U enhances the expression of specific genes by stabilizing mRNA (2007)
  • Author(s): Yugami M, Kabe Y, Yamaguchi Y, Wada T, Handa H
  • Journal Title: FEBS Lett. 581 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Bone marrow retaining colitogenic CD4+ T cells may be a pathogenic reservoir for chronic colitis. (2007)
  • Author(s): Nemoto Y, Watanabe M, et. al.
  • Journal Title: Gastroenterology. 132 Pages: 176-189
  • Peer Reviewed
• [Journal Article] Reciprocal targeting of Hath1 and β-catenin by Wnt-glycogen synthase kinase 3β in human colon cancer. (2007)
  • Author(s): Tsuchiya K, Watanabe M, et. al.
  • Journal Title: Gastroenterology. 132 Pages: 208-220
  • NAID: 40015644487
  • Peer Reviewed
• [Journal Article] Extracorporeal elimination of TNF-α-producing CD14^<dul>l CD16^+ monocytes in leukocytapheresis therapy for ulcerative colitis (2007)
  • Author(s): Kanai T, Watanabe M, et. al.
  • Journal Title: Inflamm Bowel Dis. 13 Pages: 284-290
  • Peer Reviewed
• [Journal Article] IL-7 is essential for the development and the persistence of chronic colitis. (2007)
  • Author(s): Totsuka T, Watanabe M, et. al.
  • Journal Title: J Immunol. 178 Pages: 4737-4748
  • Peer Reviewed
• [Journal Article] Intestinal lamina propria retaining CD4+CD25+regulatory T cells is a suppressive site of intestinal inflammation. (2007)
  • Author(s): Makita S, Watanabe M, et. al.
  • Journal Title: J Immunol. 178 Pages: 4937-4946
  • Peer Reviewed
• [Journal Article] Crosstalk between Wnt and Notch signaling in intestinal epithelial cell fatedecision. (2007)
  • Author(s): Nakamura T, Watanabe M, et. al.
  • Journal Title: J Gastroenterol. 42 Pages: 705-710
  • NAID: 10019816553
  • Peer Reviewed
• [Journal Article] Blockade of NKG2D signaling prevents the development of murine CD4+T cell-mediated colitis. (2007)
  • Author(s): Ito Y, Watanabe M, et. al.
  • Journal Title: Am J Physiol GI & Liver. 394
  • Peer Reviewed
• [Journal Article] Current pharmacologic therapies and emerging alternatives in the treatment of ulcerative colitis. (2007)
  • Author(s): Nakamura T, Watanabe M, et. al.
  • Journal Title: Digestion. 77 Pages: 36-41
  • Peer Reviewed
• [Journal Article] A single polymorphic amino acid on Toxoplasma gondii kinase ROP16 determines the direct and strain-specific activation of Stat3
  • Author(s): Yamamoto M, Standley DM, Takashima S, Saiga H, Okuyama M, Kayama H, Kubo E, Ito H, Takaura M, Matsuda T, Soldati-Farve D, Takeda K
  • Journal Title: J.Exp.Med. (in press)
  • Peer Reviewed
• [Journal Article] Identification of a primary target of thalidomide teratogenicity
  • Author(s): Ito T, Ando H, Suzuki T, Ogura T, Hotta K, Imamura Y, Yamaguchi Y, Handa H
  • Journal Title: Science (in press)
  • Peer Reviewed
• [Journal Article] Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide (PACAP) gene
  • Author(s): Tominaga A, Sugawara H, Futagawa T, Inoue K, Sasaki K, Minamino N, Hatakeyama M, Handa H, Miyata A
  • Journal Title: Genes Cells (in press)
  • Peer Reviewed
• [Journal Article] Identification of dynamin-2-mediated endocytosis as a new target of osteoporosis drugs, bisphosphonates
  • Author(s): Masaike Y, Takagi T, Hirota M, Yamada J, Ishihara S, Yung T.M.C., Inoue T, Sawa, C, Sagara H, Sakamoto S, Kabe Y, Takahashi Y, Yamaguchi Y,Handa H
  • Journal Title: Mol.Pharmacol (in press)
  • Peer Reviewed
• [Journal Article] Calcium-bridge Triggers capsid Disassembly in the Cell Entry Process of Simian Virus 40
  • Author(s): Kawano M, Xing L, Tsukamoto H, Inoue T, Handa H, Holland R
  • Journal Title: Biochem.Mol.Biol (in press)
  • Peer Reviewed
• [Journal Article] DSIF, the Paf1 complex, and Tat-SF1 have non-redundant, cooperative roles in RNA polymerase II elongation
  • Author(s): Chen Y, Yamaguchi Y, Tsugeno Y, Yamamoto J, Yamada T, Nakamura M, Hisatake K, Handa H
  • Journal Title: Genes Dev. (in press)
  • Peer Reviewed
• [Journal Article] Cellular dynamics of the negative transcription elongation factor NELF
  • Author(s): Yung T M.C., Narita T, Komori T, Yamaguchi Y, Handa H
  • Journal Title: Exp.Cell Res. (in press)
  • Peer Reviewed
• [Journal Article] Mono-(2-ethylhexyl) phthalate targets glycogen debranching enzyme and affects glycogen metabolism in rat testis
  • Author(s): Kuramori C, Hase Y, Hoshikawa K, Watanabe K, Nishi T, Hishiki T, Soga T, Nashimoto A, Kabe Y, Yamaguchi Y, Watanabe H, Kataoka K, Suematsu M, Handa H
  • Journal Title: Toxicological Sciences (in press)
  • Peer Reviewed
• [Presentation] 炎症性腸疾患における Notch シグナル異常と分子標的の可能性 (2009)
  • Author(s): 渡辺守
  • Organizer: 第37回日本臨床免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-14
• [Presentation] 「消化器疾患のトピックス」IBD (2009)
  • Author(s): 渡辺守
  • Organizer: 第51回日本消化器病学会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-17
• [Presentation] GSK3 inhibitor induces the intestinal differentiation by the protein stabilization of Atoh1 (2009)
  • Author(s): Tsuchiya K, Okamoto R, Nakamura T, Watanabe M
  • Organizer: DDW2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-06-02
• [Presentation] GSK3 inhibitor induces the intestinal differentiation by the protein stabilization of Atohl. (2009)
  • Author(s): Tsuchiya K, Watanabe M, et al.
  • Organizer: DDW2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-06-02
• [Presentation] Notch1 activation promptes goblet cell depletion and expression of PLA2G2A in the inflamed mucosa of ulcerative colitis (2009)
  • Author(s): Okamoto R
  • Organizer: DDW2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-06-01
• [Presentation] Systemic, but not intersinal, IL-7 is essential for the development and persistence of Chronic Colitis (2009)
  • Author(s): Totsuka T
  • Organizer: DDW2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-05-31
• [Presentation] 炎症性腸疾患と発癌 (2009)
  • Author(s): 渡辺守
  • Organizer: 第106回日本内科学会総会・講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-10
• [Presentation] Pathogenesis of Inflammatory Bowel Disease : Current Understanding (2009)
  • Author(s): Watanabe M
  • Organizer: Asia Pacific Working Group Inaugural Meeting on IBD
  • Place of Presentation: Chaina
  • Year and Date: 2009-03-07
• [Presentation] Innate immune responses at the intestinal mucosa (2009)
  • Author(s): Kiyoshi Takeda
  • Organizer: The first CSI/JSI/KAI Joint Symposium on Immunology
  • Place of Presentation: Shanghai, China
• [Presentation] Innate immune responses at the intestinal mucosa (2009)
  • Author(s): Kiyoshi Takeda
  • Organizer: The 2009 Fall Conference of the Korean Association of Immunologists
  • Place of Presentation: Seoul, Korea
• [Presentation] ATP from commensal bacteria induces Th17 cell development in the intestine (2009)
  • Author(s): Kiyoshi Takeda
  • Organizer: Regulation of innate immunity
  • Place of Presentation: Seoul, Korea
• [Presentation] 腸管粘膜に特有の自然免疫系細胞の機能 (2009)
  • Author(s): 竹田潔
  • Organizer: 第39回日本免疫学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 自然免疫系の活性制御と免疫疾患(特別講演) (2009)
  • Author(s): 竹田潔
  • Organizer: 第51回日本小児血液学会
  • Place of Presentation: 東京
• [Presentation] Commensal bacteria-derived ATP mediates development of intestinal Th17 cells (2009)
  • Author(s): Kiyoshi Takeda
  • Organizer: RCAI-JSI International Symposium on Immunology 2009
  • Place of Presentation: 横浜
• [Presentation] 炎症性腸疾患における粘膜免疫異常と上皮分化・再生障害の接点 (2008)
  • Author(s): 渡辺守
  • Organizer: 第29回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] 自然免疫系と炎症性腸疾患 (2008)
  • Author(s): 竹田潔
  • Organizer: 第29回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] 腸内フローラと炎症性腸疾患 (2008)
  • Author(s): 竹田潔
  • Organizer: 第12回腸内細菌学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-13
• [Presentation] Notch signaling suppresses the transcriptional activity of Hath1 Gene, resulting in the undifferentiated form of human intestinal epithelial cells (2008)
  • Author(s): Tsuchiya K, Inoue K, Aragaki M, Okamoto R, Nakamura T, Kanai T, Watanabe M
  • Organizer: DDW2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-05-20
• [Presentation] Notch signaling suppresses the transcriptional activity of Hath1 Gene, resulting in the undifferentiated form of human intestinal epithelial cells. (2008)
  • Author(s): Tsuchiya K, Watanabe M
  • Organizer: DDW 2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-05-20
• [Presentation] A mechanism for development of intestinal Th17 cells causing intestinal inflammation (2008)
  • Author(s): Kiyoshi Takeda, Koji Atarashi, Kenya Honda
  • Organizer: The 7^<th> Sino-Japan Joint Conference for Cancer Research
  • Place of Presentation: Guangzhou, China
• [Presentation] NFAT is responsible for TLR-independent innate immune responses to a protozoan parasite (2008)
  • Author(s): Kiyoshi Takeda, Hisako Kayama, Masahiro Yamamoto
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Commensal bacteria-derived ATP mediates Th17 cell development in the intestinal lamina propria (Symposium) (2008)
  • Author(s): Kiyoshi Takeda, Koji Atarashi, Kenya Honda
  • Organizer: 第37回日本免疫学会学術集会
  • Place of Presentation: 京都
• [Presentation] Regulation of innate immune responses (2007)
  • Author(s): Kiyoshi Takeda
  • Organizer: The 9^<th> International Colooquium on Paratuberculosis
  • Place of Presentation: Tsukuba
  • Year and Date: 2007-10-29
• [Presentation] Regulation of inflammatory responses by nuclear IkB proteins (2007)
  • Author(s): Kiyoshi Takeda
  • Organizer: Regulation of inflammatory responses by nuclear IkB proteins
  • Place of Presentation: Sendai
  • Year and Date: 2007-10-25
• [Presentation] 自然免疫系による結核感染防御機構 (2007)
  • Author(s): 竹田潔
  • Organizer: 第60回日本細菌学会九州支部総会
  • Place of Presentation: 長崎
  • Year and Date: 2007-10-12
• [Presentation] 自然免疫シグナルの制御機構 (2007)
  • Author(s): 竹田潔
  • Organizer: 第28回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2007-08-02
• [Presentation] Stabilization of Atoh1 protein induces Mucin2 gene expression in human coloncancer cells. (2007)
  • Author(s): Tsuchiya K, Watanabe M
  • Organizer: DDW 2007.
  • Place of Presentation: Washington, D.C
  • Year and Date: 2007-05-23
• [Presentation] Ulcerative colitis : A disorder of epithelial cell differentiation? (2007)
  • Author(s): 渡辺守
  • Organizer: 第93回日本消化器病学会 (4th Joint Meeting of the Japanese Society of Gastroenterology and the American Gastroenterological Association)
  • Place of Presentation: 青森
  • Year and Date: 2007-04-21
• [Presentation] Emerging issues in inflammatory bowel diseases (2007)
  • Author(s): 渡辺守
  • Place of Presentation: 神戸
• [Presentation] Emerging issues in inflammatory bowel diseases. (2007)
  • Author(s): Watanabe M
  • Organizer: APDW 2007.
  • Place of Presentation: 神戸