Construction of a homozygous mutant embryonic stem cell bank

• Project/Area Number: 19300146
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: HORIE Kyoji Osaka University, 医学系研究科, 准教授 (30333446)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: ES細胞 / マウス / トランスポゾン / レトロウイルス / 遺伝子 / Bloom / 変異体 / ゲノム / バオテクノロジー / バイオテクノロジー

Research Abstract

Mutagenesis of mouse embryonic stem (ES) cells provides a powerful resource for functional analyses of genes. Emerging cell culture techniques for directed differentiation of ES cells will further accelerate this trend. The diploid nature of the mammalian genome often hampers this process because both alleles need to be mutated for each gene. In the present study, we develop a novel method to rapidly mutate diploid genome of mouse ES cells and generate homozygous mutants. This method will facilitate gene function study in tissue culture systems.

Research Products

• [Presentation] 両アレル変異導入法を用いたES細胞の未分化性制御機構の解析 (2011)
  • Author(s): 堀江恭二、國府力、吉田純子、竹田潤二
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] 両アレル変異導入法を用いたES細胞の未分化性制御機構の解析 (2011)
  • Author(s): 堀江恭二
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] A homozygous mutant mouse embryonic stem cell bank readily applicable for phenotype-driven genetic screening. (2010)
  • Author(s): 堀江恭二, 國府力, 吉田純子, 竹田潤二
  • Organizer: Instenational Society for Stem Cell Research (ISSCR) 8th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2010-06-17
• [Presentation] A homozygous mutant mouse embryonic stem cell bank readily applicable for phenotype driven genetic screening (2010)
  • Author(s): 堀江恭二
  • Organizer: International Society for Stem Cell Research (ISSCR) 8^<th> Annual Meeting
  • Place of Presentation: San Francisco, USA.
  • Year and Date: 2010-06-17
• [Remarks] ホームページ等
  • URL: http://www.med.osaka-u.ac.jp/pub/mr-envi/www/japanese/homo/index.html