Mesenchymal stem cells dynamism in microgravity environment.

• Project/Area Number: 19300191
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Hiroshima University
• Principal Investigator: YUGE Rui Hiroshima University, 大学院・保健学研究科, 教授 (20263676)
• Co-Investigator(Kenkyū-buntansha): TAHARA Hidetoshi 広島大学, 大学院・医歯薬学総合研究科, 教授 (00271065) ; KAJIUME Teruyuki 広島大学, 大学院・医歯薬学総合研究科, 助教 (40278924)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
• Keywords: 微小重力環境 / 間葉系幹細胞 / 細胞骨格 / テロメア / 再生医療

Research Abstract

Microgravity environment expand mesenchymal stem cells populations that were maintained undifferentiated state. We showed microgravity environment caused polymeric decreased actin filament related to cytoskeleton proteins and decrease of number of focal contacts. Undifferentiating cells in microgravity environment as a result that decreased of signal by physical stimulation from the cell surface into a nucleus, and gene expression changed. Telomere length remained during culturing was considered in microgravity environment.

Research Products

• [Journal Article] LIF-free embryonic stem cell culture in simulated microgravity. (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Kajiume T., Matsumoto M., Yuge L
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] 重力が生体に与える影響 (2009)
  • Author(s): 弓削類
  • Journal Title: 理学療法 26 Pages: 585-589
• [Journal Article] LIF-free embryonic stem cell culture in simulated microgravity (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Kajiume T., Matsumoto M., Yuge L.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Impact of the microgravity environment in a three-dimensional clinostat on osteoblast-and osteoclast-like cells. (2008)
  • Author(s): Makihira S., Kawahara Y., Yuge L., Mine Y., Nikawa H
  • Journal Title: Cell Biol Int 32 Pages: 1176-1181
  • Peer Reviewed
• [Journal Article] Impact of the microgravity environment in a three dimensional clinostat on osteoblast and osteoclast like cells. (2008)
  • Author(s): Makihira S., kawahara Y., Yuge L., Mine Y., Nikawa H.
  • Journal Title: Cell Bioliology International 32 Pages: 1176-1181
  • Peer Reviewed
• [Presentation] 微小重力環境と幹細胞の未分化維持-マウスES細胞での検討- (2010)
  • Author(s): 河原裕美, 松本昌也, 猪村剛史, 松本昌泰, 弓削類
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] 模擬微小重力環境下での細胞塊形成と神経分化能 (2010)
  • Author(s): 猪村剛史, 松本昌也, 河原裕美, 弓削類
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] Simulated microgravity: a novel approach to embryonic stem cell culture. (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M, Imura T., Kajiume T., Takeda M., Matsumoto M., Yuge L
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-07
• [Presentation] Simulated microgravity : a novel approach to embryonic stem cell culture (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M, Imura T., Kajiume T., Takeda M., Matsumoto M., Yuge L.
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-07
• [Presentation] Transplantation of bone marrow stromal cells cultured under simulated microgravity into a spinal cord injury rat. (2009)
  • Author(s): Takeda M., Magaki T., Sasaki A., Manabe T., Matsumoto M., Kawahara Y., Yuge L., Kurisu K
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-06
• [Presentation] Transplantation of bone marrow stromal cells cultured under simulated microgravity into a spinal cord injury rat (2009)
  • Author(s): Takeda M., Magaki T., Sasaki A., Manabe T., Matsumoto M., Kawahara Y., Yuge L., Kurisu K.
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 25th Annual Meeting NASA)
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-11-06
• [Presentation] Microgravity facilitates stem cell proliferation and neural differentiation after transplantation. (2009)
  • Author(s): Yuge L
  • Organizer: 6th Annual World Congress for Brain Mapping and Image Guided Therapy-International Brain Mapping and Intraoperative Surgical planning Sossiety (IBMISPS)
  • Place of Presentation: Harvard Medical School, Boston, USA
  • Year and Date: 2009-08-28
• [Presentation] Microgravity facilitates stem cell proliferation and neural differentiation after transplantation (2009)
  • Author(s): 弓削類
  • Organizer: 6th Annual World Congress for Brain Mapping and Image Guided Therapy-International Brain Mapping and Intraoperative Surgical planning Sossiety (IBMISPS)
  • Place of Presentation: Harvard Medical School, Boston, USA
  • Year and Date: 2009-08-28
• [Presentation] Simulated microgravity enables LIF-free culture in mouse embryonic stem cells. (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Ogawa K., Kajiume T., Takeda M., Magaki T., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L
  • Organizer: 7th International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-09
• [Presentation] Simulated microgravity enables LIF-free culture in mouse embryonic stem cells (2009)
  • Author(s): Kawahara Y., Manabe T., Matsumoto M., Ogawa K., Kajiume T., Takeda M., Magaki T., Yamashita H., Takahashi T., Aoki S., Sueda Y., Matsumoto M., Yuge L.
  • Organizer: 7th International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-09
• [Presentation] ラットにおける脊髄損傷後の微小重力培養骨髄由来細胞移植 (2009)
  • Author(s): 真鍋朋誉, 武田正明, 松本昌也, 小川和幸, 河原裕美, 弓削類
  • Organizer: 第44回日本理学療法学学術大会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-30
• [Presentation] 脊髄損傷に対する微小重力培養骨髄由来細胞移植 (2009)
  • Author(s): 武田正明, 籬拓郎, 真鍋朋誉, 弓削類, 栗栖薫
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-06
• [Presentation] 神経再生における微小重力環境で培養した骨髄間質細胞の有用性 (2009)
  • Author(s): 弓削類, 田原栄俊, 河原裕美, Anil D. Kulkarni, Lewis Romer
  • Organizer: 第25回宇宙利用シンポジウム
  • Place of Presentation: 相模原
  • Year and Date: 2009-01-15
• [Presentation] Stem cell culture in microgravity. (2008)
  • Author(s): Yuge L
  • Organizer: BIT's 1st Annual World Congress of Regenerative Medicine & Stem Cell 2008
  • Place of Presentation: Foshan, China
  • Year and Date: 2008-12-02
• [Presentation] Stem cell culture in microgravity. (2008)
  • Author(s): Yuge L.
  • Organizer: BIT's Ist Annual World Congress of Regenerative Medicine & Stem Cell 2008
  • Place of Presentation: Foshan, China
  • Year and Date: 2008-12-02
• [Presentation] Microgravity inhibits neural differentiation while sustaining the capability of differentiation. (2008)
  • Author(s): Yuge L., Sasaki A., Takeda M., Magaki T., Wu SL., Kawahara Y., Yoshimoto R., Manabe T., Ogawa K., Matsumoto M., Kajiume T
  • Organizer: 6th International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2008-06-11
• [Presentation] 微小重力環境を利用した骨髄由来細胞の神経分化制御と治療効果 (2008)
  • Author(s): 佐々木輝, 呉樹亮, 吉元玲子, 真鍋朋誉, 井川英明, 小川和幸, 松本昌也, 武田正明, 河原裕美, 弓削類
  • Organizer: 第43回日本理学療法学学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-15
• [Presentation] 微小重力環境を利用した骨髄由来細胞の神経分化制御と治療効果 (2008)
  • Author(s): 佐々木輝, 呉樹亮, 吉元玲子, 真鍋朋誉, 井川英明, 小川和幸, 松本昌也, 武田正明, 河原裕美, 弓削類
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-13
• [Presentation] Stem cell culture in microgravity. (2008)
  • Author(s): Yuge L
  • Organizer: 12th ADNAT convention, Symposium on Biology of Embryonic and Adult Stem Cells
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2008-02-24
• [Presentation] Stem Cell Culture in Mycrogravity (2008)
  • Author(s): Yuge L.
  • Organizer: 12th ADNAT convention, Symposium on Biology of Embryonic and Adult Stem Cells
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2008-02-24
• [Presentation] 微小重力環境における細胞の分化と老化の抑制 (2008)
  • Author(s): 弓削類, 田原栄俊, 河原裕美, Anil D. Kulkarni
  • Organizer: 第24回宇宙利用シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-01-17
• [Presentation] Microgravity inhibits neural differentiation while sustaining the capability of differentiation. (2007)
  • Author(s): Sasaki A., Takeda M., Magaki T., Wu S.L., Kawahara Y., Yoshimoto R., Manabe T., Boulenouar A., Kajiume T., Yuge L
  • Organizer: American Society for Gravitational and Space Biology (ASGSB 23rd Annual Meeting NASA)
  • Place of Presentation: Ames Research Center, Moffett Field, CA., USA
  • Year and Date: 2007-10-26
• [Presentation] Differentiation of human mesenchymal stem cells and effect of three-dimensional scaffold culture. (2007)
  • Author(s): Umeda C., Nitta J., Kawahara Y., Yoshimoto R., Kajiume T., Sasaki A., Wu S.L., Manabe T., Kanno M., Naminohira K., Nakagawa K., Fushimi T., Fujimura M., Yuge L
  • Organizer: 15th International Congress of World Confederation for Physical Therapy (WCPT)
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2007-06-05
• [Presentation] Differentiation of Human Mesenchymal Stem Cells and Effect of Three-Dimensional Scaffold Culture. (2007)
  • Author(s): Yuge L.
  • Organizer: 15th International Congress of WCPT
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2007-06-05