Study on DNA repair mechanism and mutagenesis using novel cell lines
Project/Area Number |
19310035
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
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Research Institution | Ibaraki University |
Principal Investigator |
TAUCHI Hiroshi Ibaraki University, 理学部, 教授 (70216597)
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Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | 放射線 / 突然変異 / DNA損傷修復 / 修復 / 放射線影響 / DNA損傷 |
Research Abstract |
In this study, we focused on the regulation of DNA damage repair pathways and induction of somatic mutation by radiation. We found that NBS1 regulates a novel apoptosis induction following DNA damage by disrupting the Ku70-Bax complex, which is required for activation of the mitochondrial apoptotic pathway. In addition, by using a hyper-sensitive mutation detection system, we successfully observed the reverse dose-rate effect for carbon beam at higher LET but not that at lower LET. Taken together with our previous publications on mutation induction by high LET radiation, the observation indicates that the changes in cell cycle dependence of mutation induction dependent on radiation LET should be a main cause of the reversed dose rate effect for somatic mutation. Finally, we established novel assay systems that can be applied for study on DNA repair and somatic mutation induction.
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Report
(5 results)
Research Products
(38 results)
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[Journal Article] Homologous recombination but not nucleotide excision repair plays a pivotal role in tolerance to DNA-protein crosslinks in mammalian cells.2009
Author(s)
Nakano T., Katafuchi A., Matsubara M., Terato H., Tsuboi T., Masuda, T., Tatsumoto M., Pack S.-P. Makino K., Croteau D.L., Van Houten B., Iijima K., Tauchi H., Ide H.
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Journal Title
J.Biol.Chem. 284
Pages: 27065-27076
Related Report
Peer Reviewed
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[Journal Article] Two major factors involved in the reverse dose-rate effect for somatic mutation induction are the cell cycle position and LET value2009
Author(s)
Tauchi, H., Waku, H., Matumoto, E., Yara, S., Okumura, S., Iwata,Y., Komatsu, K., Frusawa, Y., Eguchi-Kasai, K., Tachibana, A.
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Journal Title
J. Radiat. Res. 50
Pages: 441-448
NAID
Related Report
Peer Reviewed
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[Journal Article] Homologous recombination but not nucleotide excision repair plays a pivotal role in tolerance to DNA-protein crosslinks in mammalian cells2009
Author(s)
Nakano, T., Katafuchi, A., Matsubara, M., Terato, H., Tsuboi, T., Masuda, T., Tatsumoto, M., Pack, S.-P. Makino, K., Croteau, D. L., Van Houten, B., Iijima, K., Tauchi, H., Ide, H.
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Journal Title
J. Biol. Chem. 284
Pages: 27065-27076
Related Report
Peer Reviewed
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[Journal Article] NBS1 regulates a novel apoptotic pathway through Bax activation2008
Author(s)
Iijima, K., Muranaka, C., Kobayashi, J., Sakamoto, S., Komatsu, K., Matsuura, S., Kubota, N., Tauchi, H.
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Journal Title
DNA Repair 7
Pages: 1705-1716
Related Report
Peer Reviewed
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