| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Research Abstract |
In this study, we focused on the regulation of DNA damage repair pathways and induction of somatic mutation by radiation. We found that NBS1 regulates a novel apoptosis induction following DNA damage by disrupting the Ku70-Bax complex, which is required for activation of the mitochondrial apoptotic pathway. In addition, by using a hyper-sensitive mutation detection system, we successfully observed the reverse dose-rate effect for carbon beam at higher LET but not that at lower LET. Taken together with our previous publications on mutation induction by high LET radiation, the observation indicates that the changes in cell cycle dependence of mutation induction dependent on radiation LET should be a main cause of the reversed dose rate effect for somatic mutation. Finally, we established novel assay systems that can be applied for study on DNA repair and somatic mutation induction.
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