Development of testicular cell system with cell-cell interactions

• Project/Area Number: 19310040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: University of Toyama
• Principal Investigator: TABUCHI Yoshiaki University of Toyama, 生命科学先端研究センター, 准教授 (20322109)
• Co-Investigator(Kenkyū-buntansha): TAKASAKI Ichiro 富山大学, 生命科学先端研究センター, 助教 (00397176)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 内分泌かく乱物質 / 細胞間相互作用 / 精巣細胞システム / Sertoli細胞 / Leydig細胞 / 血液―精巣関門 / 密着結合 / 小胞体ストレス / DJ-1 / Leydig TTE1細胞 / 遺伝子発現 / 不死化セルトリTTE3細胞 / 遺伝子ネットワーク / 不死化ライデイヒTTEl細胞

Research Abstract

TTE3セルトリ細胞とTTE1ライディヒ細胞を共培養した時,セルトリ細胞において数多くの遺伝子の発現が変動した.本細胞システムにおいて,セルトリ細胞の細胞分化の指標であるトランスフェリンの発現が上昇し,細胞周期関連遺伝子であるサイクリンD1の発現が減少した.さらに,コンピューターを用いた遺伝子発現解析により,細胞間相互作用に関連するユニークな遺伝子ネットワークを明らかにした.

Research Products

• [Journal Article] Fetal and neonatal exposure to three typical environmental chemicals with different mechanisms of action: Mixed exposure to phenol, phthalate, and dioxin cancels the effects of sole exposure on mouse midbrain dopaminergic nuclei (2009)
  • Author(s): Tanida T., Warita K., Ishihara K., Fukui S., Mitsuhashi T., Sugawara T., Tabuchi Y., Nanmori T., Qi W.M., Inamoto T., Yokoyama T., Kitagawa H., Hoshi N.
  • Journal Title: Toxicol Lett Vol.189 Pages: 40-47
  • Peer Reviewed
• [Journal Article] Gene networks involved in the apoptosis induced by hyperthermia in human lymphoma U937 cells (2009)
  • Author(s): Furusawa Y., Tabuchi Y., Takasaki I., Wada S., Ohtsuka K., Kondo T.
  • Journal Title: Cell Biol. Int Vol.33 Pages: 1253-1262
  • Peer Reviewed
• [Journal Article] Fetal and neonatal exposure to three typical environmental chemicals with different mechanisms of action : Mixed exposure to phenol, phthalate, and dioxin cancels the effects of sole exposure on mouse midbrain dopaminergic nuclei (2009)
  • Author(s): Tanida, et al.
  • Journal Title: Toxicol.Lett. 189 Pages: 40-47
  • Peer Reviewed
• [Journal Article] Gene networks involved in the apoptosis induced by hyperthermia in human lymphoma U937 cells (2009)
  • Author(s): Furusawa, et al.
  • Journal Title: Cell Biol.Int. 33 Pages: 1253-1262
  • Peer Reviewed
• [Journal Article] Genes and genetic networks responsive to mild hyperthermia in human lymphoma U937 cells (2008)
  • Author(s): Tabuchi Y., Takasaki I., Wada S., Zhao Q.L., Hori T., Nomura T., Ohtsuka K., Kondo T.
  • Journal Title: Int. J. Hyperthermia Vol.24 Pages: 613-62
  • Peer Reviewed
• [Journal Article] Establishment and characterization of tracheal epithelial RTEC11 cells from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen (2008)
  • Author(s): Tabuchi Y., Doi T., Takasaki I., Takahashi R., Ueda M., Suzuki Y., Obinata M.
  • Journal Title: Cell Biol. Int Vol.32 Pages: 1344-1352
  • Peer Reviewed
• [Journal Article] Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells (2008)
  • Author(s): Tabuchi Y., Takasaki I., Zhao Q.L., Wada S., Hori T., Feril L.B. Jr., Tachibana K., Nomura T., Kondo T.
  • Journal Title: Cancer Lett Vol.270 Pages: 286-294
  • Peer Reviewed
• [Journal Article] Genes and genetic networks responsive to mild hyperthermia in human lymphoma U937 cells (2008)
  • Author(s): Tabuchi, et al.
  • Journal Title: Int. J. Hyperthermia 24 Pages: 613-622
  • Peer Reviewed
• [Journal Article] Establishment and characterization of tracheal epithelial RTECll cells from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen (2008)
  • Author(s): Tabuchi, et al.
  • Journal Title: Cell Biol. Int. 32 Pages: 1344-1352
  • Peer Reviewed
• [Journal Article] Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells (2008)
  • Author(s): Tabuchi, et al.
  • Journal Title: Cancer Lett. 270 Pages: 286-294
  • Peer Reviewed
• [Journal Article] Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12 (2007)
  • Author(s): Takasaki I., Takarada S., Fukuchi M., Yasuda M., Tsuda M., Tabuchi Y
  • Journal Title: J. Cell. Biochem Vol.102 Pages: 1472-1485
  • Peer Reviewed
• [Journal Article] Genetic networks in nonpermissive temperature-induced cell differentiation of Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen (2007)
  • Author(s): Tabuchi Y., Takasaki I., Suto A., Kondo T., Suzuki Y., Obinata M.
  • Journal Title: Cell Biol. Int Vol.31 Pages: 1231-1236
  • Peer Reviewed
• [Journal Article] Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells (2007)
  • Author(s): Salunga T.L., Tabuchi Y., Takasaki I., Feril L.B. Jr., Zhao Q.L., Ohtsuka K., Tsuneyama K., Kondo T.
  • Journal Title: Int. J. Hyperthermia Vol.23 Pages: 529-537
  • Peer Reviewed
• [Journal Article] Genetic networks in nonpermissive temperature-induced cell differentiation of Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen (2007)
  • Author(s): Tabuchi, Y., Takasaki, I., et. al.
  • Journal Title: Cell Biol. Int 31 Pages: 1231-1236
  • Peer Reviewed
• [Journal Article] Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells (2007)
  • Author(s): Salunga, T. L., Tabuchi, Y., Takasaki, I., et. al.
  • Journal Title: Int. J. Hyperthermia 23 Pages: 529-537
  • Peer Reviewed
• [Journal Article] Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12 (2007)
  • Author(s): Takasaki, I., et. al.
  • Journal Title: J. Cell. Biochem 102 Pages: 1472-1485
  • Peer Reviewed
• [Presentation] 精巣セルトリ細胞における小胞体ストレスに対するDJ-1の細胞保護作用 (2009)
  • Author(s): 土井健司, 高崎一朗, 平敬宏, 有賀寛芳, 近藤隆, 田渕圭章
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都市
• [Presentation] 精巣セルトリ細胞のビスフェノールAによる細胞死に関与するbZIP転写因子群 (2008)
  • Author(s): 高崎一朗, 林淳美, 近藤隆, 田渕圭章
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜市
• [Presentation] 外因性エストロゲン様化学物質がLeydig細胞株TTE1のステロイドホルモン産生系遺伝子発現に与える影響とヒストンアセチル化解析 (2007)
  • Author(s): 割田克彦, 菅原照夫, 田渕圭章, 三觜友子, 松本由樹, 三木崇範, 石原可奈, 谷田任司, 横山俊史, 竹内義喜, 北川浩, 星信彦
  • Organizer: 環境ホルモン学会第10回研究発表会
  • Place of Presentation: 大宮市
  • Year and Date: 2007-12-10
• [Presentation] 外因性エストロゲン様化学物質がLeydig細胞株TTE1のステロイドホルモン産生系遺伝子発現に与える影響とヒストンアセチル化解析 (2007)
  • Author(s): 割田 克彦, 菅原 照夫, 田渕 圭章, ら
  • Organizer: 環境ホルモン学会第10回研究発表会
  • Place of Presentation: 埼玉県大宮市
  • Year and Date: 2007-12-10
• [Remarks]
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