Analyses and application of the mechanism for an efficient dechlorination by the bacterial symbiotic system.

• Project/Area Number: 19310049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Shizuoka University (2008-2009); Toyohashi University of Technology (2007)
• Principal Investigator: FUTAMATA Hiroyuki Shizuoka University, 工学部, 准教授 (50335105)
• Co-Investigator(Kenkyū-buntansha): HIRAISHI Akira 豊橋技術科学大学, エコロジー工学系, 教授 (40283486)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 環境修復技術 / バイオレメディエーション / 微生物の共生系 / 還元的脱塩素化 / "Dehalococcoides"細菌群 / 微生物間相互作用 / 水素生産菌 / 種間水素伝達系 / Dehalococcoides細菌群 / 水素生成細菌 / 電子供与体

Research Abstract

土壌を接種源とする集積培養物TUT2264は、テトラクロロエテン(PCE)をジクロロエテン(DCEs)にまで脱塩素化する能力を発揮した。16S rRNA遺伝子を標的とした群集構造解析では、"Dehalococcoides"とAcidaminobacter属細菌の存在が確認された。また8種のRdhAsが見出され、転写レベルからもPCEおよびトリクロロエテンの脱塩素化が確認された一方で、分解活性が見られないDCEsおよびビニルクロライドによって転写が促進されていることが示された。クエン酸およびグルタミン酸がPCEの脱塩素化にとって有効な電子供与体となり得ることが示された。ヘッドスペース中の初期水素濃度(0%(v/v)と80%(v/v)がPCE脱塩素化に及ぼす影響を解析した結果、水素生産細菌のAcidaminobacter属細菌菌密度に差異は見られなかったが、"Dehalococcoides"においては0%の方が約100倍高く、分解活性も3倍程高かった。以上の結果は、異属微生物間の水素伝達系を良好に制御することで効果的な脱塩素化が可能なことを示唆している。

Research Products

• [Journal Article] Removal of polychlorinated dioxins by semi-aerobic fed-batch composting with biostimulation of "Dehalococcoides". (2010)
  • Author(s): Narihiro, T.S. Kaiya, H. Futamata, A. Hiraishi
  • Journal Title: J. Biosci. Biotechnol 109(3) Pages: 249-256
  • NAID: 110007612432
• [Journal Article] Removal of polychlorinated dioxins by semi-aerobic fed-batch composting with biostimulation of "Dehalococcoides" (2010)
  • Author(s): Narihiro, T.S.Kaiya, H.Futamata, A.Hiraishi.
  • Journal Title: J.Biosci.Biotechnol. 109 Pages: 249-256
  • NAID: 110007612432
  • Peer Reviewed
• [Journal Article] Relationship between reductive-dehalogenase-homologous genes transcriptional level and dechlorinating activity in "Dehalococcoides"-containing tetrachloroethene-dechlorinating enrichment culture TUT2264. (2009)
  • Author(s): Futamata, H., S. Kaiya, M. Sugawara, A. Hiraishi
  • Journal Title: Microbes and Environ 24 Pages: 330-337
• [Journal Article] Relationship between reductive-dehalogenase-homologous genes transcriptional level and dechlorinating activity in "Dehalococcoides"-containing tetrachloroethene-dechlorinating enrichment culture TUT2264. (2009)
  • Author(s): Futamata, H., S.Kaiya, M.Sugawara, A.Hiraishi.
  • Journal Title: Microbes and Environments 24 Pages: 330-337
  • Peer Reviewed
• [Journal Article] Biodiversity and ecophysiology of anaerobic dechlorinating microorganisms: implications for bioremediation. (2007)
  • Author(s): Futamata, H., A. Hiraishi
  • Journal Title: Current Trend Microbiol 3 Pages: 63-92
• [Journal Article] Reductive dechlorination of chloroethenes by Dehalococcoides-containing cultures enriched from a polychlorinated-dioxin-contaminated mirocosm. (2007)
  • Author(s): Futamata, H., N. Yoshida, T. Kurogi, S. Kaiya, A. Hiraishi
  • Journal Title: The ISME J 1 Pages: 471-479
• [Journal Article] Reductive dechlorination of chloroethenes by Dehalococcoides-containing cultures enriched from a polychlorinated-dioxin-contaminated microcosm. (2007)
  • Author(s): Futamata, H., N.Yoshida, T.Kurogi, S.Kaiya and A.Hiraishi
  • Journal Title: The ISME Journal 1 Pages: 471-479
  • Peer Reviewed
• [Presentation] Understanding of microbial ecosystem and its application. (2010)
  • Author(s): Hiroyuki Futamata
  • Organizer: Pusan National University-Shizuoka University joint symposium and Graduate students forum for Promotion of the DDP
  • Place of Presentation: 静岡県浜松市
  • Year and Date: 2010-02-05
• [Presentation] Understanding of microbial ecosystem and its application (2010)
  • Author(s): Hiroyuki FUTAMATA
  • Organizer: Pusan National University-Shizuoka University joint symposium and Graduate students forum for Promotion of the DDP.
  • Place of Presentation: 静岡県浜松市
  • Year and Date: 2010-02-05
• [Presentation] "Dehalococcoides"集積培養物TUT2264の塩化エテン類脱塩素化に及ぼす水素濃度の影響 (2009)
  • Author(s): 上田紘也、山本脩二、飯倉智弘、二又裕之
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島県西条市、広島大学
  • Year and Date: 2009-11-21
• [Presentation] "Dehalococcides" 集積培養物TUT2264の塩化エテン類脱塩素化に及ぼす水素濃度の影響 (2009)
  • Author(s): 上田紘也
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島県西条市
  • Year and Date: 2009-11-21
• [Presentation] 微生物生態系の潜在的浄化能力と相互作用 (2009)
  • Author(s): 二又裕之
  • Organizer: DNA組換え講演会
  • Place of Presentation: 愛知県名古屋市名城大学
  • Year and Date: 2009-11-19
• [Presentation] 微生物生態系の潜在的浄化能力と相互作用 (2009)
  • Author(s): 二又裕之
  • Organizer: 名城大学DNA組換え講演会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-11-19
• [Presentation] 環境浄化とエネルギー生産複 (2009)
  • Author(s): 二又裕之
  • Organizer: テクノサロン浜松
  • Place of Presentation: 静岡県浜松市
  • Year and Date: 2009-06-08
• [Presentation] Characterization of the dechlorinating enrichment culture TUT2264 with the different organohalides. (2009)
  • Author(s): Akemi Nose, Shinichi Kaiya, Takashi Kurogi, Hiroyuki Futamata, Akira Hiraishi
  • Organizer: The 1^<st> international symposium on green technology for global carbon cycle in Asia
  • Place of Presentation: 新潟県長岡市(長岡技術科学大学)
  • Year and Date: 2009-03-23
• [Presentation] Characterization of the dechlorinating enrichment culture TUT2264 with the different organohalidesアジア・グリーンテック開発センター「アジア地域における炭素循環グリーンテクノロジー」 (2009)
  • Author(s): Akemi Nose, Shinichi Kaiya, Takashi Kurogi, Hiroyuki Futamata, Akira Hiraishi
  • Organizer: The 1st international symposium on green technology for global carbon cycle in Asia
  • Place of Presentation: 長岡技術科学大学
• [Presentation] Characterization of symbiotic dechlorinating consortium TUT2264 (2007)
  • Author(s): 二又 裕之、山崎 亨友、平石 明
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 愛媛県松山市(愛媛大学)
  • Year and Date: 2007-09-15
• [Presentation] Effect of electron donors on hydrogen produce from dechlorinating consortium TUT2264 (2007)
  • Author(s): 山崎 亨友、平石 明、二又 裕之
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 愛媛県松山市(愛媛大学)
  • Year and Date: 2007-09-15
• [Presentation] Characterization of symbiotic dechlorinating consortium TUT2264 (2007)
  • Author(s): 二又裕之, 山崎亨友, 平石明
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 愛媛県松山市、愛媛大学
• [Presentation] Akira Hiraishi Effects of different electron donors on hydrogen production in the dechlorinating consortium TUT2264 (2007)
  • Author(s): Yukitomo Yamasaki, Hiroyuki Futamata, Mariko Sugawara
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 愛媛県松山市、愛媛大学
• [Book] 微生物機能を活用した汚染環境修復技術～微生物生態系の解明と活用に向けて～ (2008)
  • Author(s): 藤江幸一編(分担
  • Publisher: コロナ社
• [Remarks]
  • URL: http://cheme.eng.shizuoka.ac.jp/~futamatalab/
• [Remarks]
  • URL: http://cheme.eng.shizuoka.ac.jp/~futamatalab/