Developmental and Evolutionary Analysis of Ancient Fish (Polypterus)

• Project/Area Number: 19310125
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: National Institute for Basic Biology
• Principal Investigator: NARUSE Kiyoshi National Institute for Basic Biology, 形質転換生物研究施設, 准教授 (50208089)
• Co-Investigator(Kenkyū-buntansha): OKABE Masataka 東京慈恵会医科大学, 医学部, 教授 (10300716)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2007: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: ゲノム進化・再編 / 古代魚 / ゲノム進化 / 条鰭類 / 肉鰭類 / ゲノム重複 / ポリプテルスセネガルス / 肺発生カスケード / エンハンサー

Research Abstract

Establishment of cultured cell line : We have been established the primary cell culture from fin, kidney and embryos of Polypterus senegalus. Initial phase of cell culture, cells were growing well. After several passages, most of cells were rabidly dying by apoptosis. This phenomenon was observed in fins, kidney and embryo. Although we have changed several culture conditions such as medium and collagen coated dish, all efforts were not successful.
Construction of fosmid library and evolutionary genetic analysis : We have constructed the Polypterus genomic library with fosmid vector system. Independent colony members of this library reached over 500,000. After PCR screening of three genes (Gcm2、Elovl5、Fbox93), single positive clone with the Elovl5 gene was identified. This result suggests the genome coverage of this fosmid library was not high enough for isolation of arbitral genes in Polypterus genome.

Research Products

• [Journal Article] Germ layer patterning in bichir and lamprey; An insight into its evolution in vertebrates. (2009)
  • Author(s): Takeuchi M, Takahashi M, Kuratani S, Okabe M, Aizawa, S
  • Journal Title: Dev Biol. 332(1) Pages: 90-102
  • Peer Reviewed
• [Journal Article] Integration of human mesenchymal stem cells into the Wolffian duct in chicken embryos. (2009)
  • Author(s): Fukui A, Yokoo T, Matsumoto K, Kawamura T, Hosoya T, Okabe M
  • Journal Title: Biochem Biophys Res Commun. 385(3) Pages: 330-335
  • Peer Reviewed
• [Journal Article] Germ layer patterning in bichir and lamprey ; An insight into its evolution in vertebrates (2009)
  • Author(s): Takeuchi M, Takahashi M, Kuratani S, Okabe M, Aizawa, S.
  • Journal Title: Dev Biol 332(1) Pages: 90-102
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Takeuchi, M., Okabe, M., Aizawa S.
  • Journal Title: The Genus Polypterus(Bichirs)(Cold Spring Harbor Laboratory Press) Pages: 447-467
• [Journal Article] (2009)
  • Author(s): Takeuchi, M., Okabe, M., Aizawa S.
  • Journal Title: Whole-Mount In Situ Hybridization of Bichir(Polypterus)Embryos(Cold Spring Harbor Laboratory Press) Pages: 458-459
• [Journal Article] (2009)
  • Author(s): Takeuchi, M., Okabe, M., Aizawa S.
  • Journal Title: Microinjection of Bichir(Polypterus)Embryos(Cold Spring Harbor Laboratory Press) Pages: 460-462
• [Journal Article] The medaka draft genome and insights into vertebrate genome evolution. (2007)
  • Author(s): Kasahara, M, Naruse, K, et. al
  • Journal Title: Nature 446 Pages: 714-719
  • Peer Reviewed
• [Journal Article] The medaka draft genome and insights into vertebrate genome evolution (2007)
  • Author(s): Kasahara, M., Naruse, K., et. al.
  • Journal Title: Nature 446 Pages: 714-719
  • Peer Reviewed
• [Presentation] 呼吸器の構造進化～脊椎動物の上陸の前後～ (2010)
  • Author(s): 岡部正隆
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Year and Date: 2010-03-28
• [Presentation] EVOLUTION OF THE VERTEBRATE PHARYNX. (2009)
  • Author(s): Masataka Okabe
  • Organizer: The 36th Congress of the International Union of Physiological Sciences.
  • Year and Date: 2009-07-31
• [Presentation] 脊椎動物の上陸と咽頭の形態進化 (2009)
  • Author(s): 岡部正隆
  • Organizer: 第50回日本組織細胞化学会総会・学術集会
• [Presentation] Transition from Aquatic to Terrestrial Life And Evolution of the Vetebrate Prarynx. (2009)
  • Author(s): Masataka Okabe
  • Organizer: THE XIth OXFORD CONFERENCE ON MODELLING AND CONTROL OF BREATHING-NEW FRONTIERS IN RESPIRATORY CONTROL-呼吸調節研究における新展開
• [Presentation] Transition from Aquatic to Terrestrial Life And Evolution of the Vetebrate Prarynx. (2009)
  • Author(s): Masakata Okabe
  • Organizer: he 3rd International Symposium of the Biodiversity & Evolution Global COE Project.
• [Presentation] Transition from Aquatic to Terrestrial Life And Evolution of the Vetebrate Prarynx (2009)
  • Author(s): Okabe, M.
  • Organizer: THE XIth OXFORD CONFERENCE ON MODELLING AND CONTROL OF BREATHING-NEW FRONTIERS IN RESPIRATORY CONTROL
  • Place of Presentation: Noh Theater, Nara Park(Nara)
• [Presentation] Transition from Aquatic to Terrestrial Life And Evolution of the Vetebrate Prarynx (2009)
  • Author(s): Okabe, M.
  • Organizer: The 3rd International Symposium of the Biodiversity & Evolution Global COE Project
  • Place of Presentation: Kyodai-Kaikan(Kyoto)
• [Presentation] Establishment of medaka full length cDNA resources-An activity of NBRP medaka- (2009)
  • Author(s): K. Naruse, Y. Yoshimura, et al.
  • Organizer: Fish genome meeting 2009
  • Place of Presentation: Hinxton、UK
• [Book] Management, and Experimental Protocols. (2009)
  • Author(s): Kinoshita, M, Murata, K, Naruse, K, Tanaka, M Medaka
  • Publisher: Biology
• [Book] A Fish Group Diverged at the Stem of Ray-Finned Fishes (Actinopterygii) (2009)
  • Author(s): Takeuchi M, Okabe M, Aizawa S. The Genus Polypterus (Bichirs)
  • Publisher: CSH Protocols
• [Book] Whole-Mount In Situ Hybridization of Bichir (Polypterus) Embryos (2009)
  • Author(s): Takeuchi M, Okabe M, Aizawa S
  • Publisher: CSH Protocols
• [Book] Microinjection of Bichir (Polypterus) Embryos (2009)
  • Author(s): Takeuchi M, Okabe M, Aizawa S
  • Publisher: CSH Protocols