| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2007: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Research Abstract |
UTKO1 was synthesized as a derivative of moverastin by Prof. Watanabe (Univ. Tokyo). We explored the mode of action of UTKO1 by using a chemical genetic approach. We found that UTKO1 inhibited EGF-induced cell migration by inhibiting Rac1 activation. To identify the molecular target of UTKO1, biotinylated UTKO1 was synthesized by Prof. Watanabe, and was used as the probe to obtain a target protein of UTKO1. As a result, 14-3-3ζ was identified as the functional target protein of UTKO1, because siRNA-mediated silencing of 14-3-3ζ expression suppressed both EGF-induced cell migration and Rac1 activation. These results suggest that 14-3-3ζ would act upstream of Rac1 activation in the process of EGF-induced cell migration, and UTKO1 inhibited cell migration possibly due to the abrogation of 14-3-3ζ function.
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