Stabilization mechanism of collagen helix by using host-guest model peptides

• Project/Area Number: 19350059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Polymer chemistry
• Research Institution: Osaka University
• Principal Investigator: OKUYAMA Kenji Osaka University, 大学院・理学研究科, 教授 (30038020)
• Co-Investigator(Kenkyū-buntansha): KANEKO Fumitoshi 大阪大学, 大学院・理学研究科, 准教授 (70214468) ; KAWAGUCHI Tatsuya 大阪大学, 大学院・理学研究科, 助教 (10314353)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2007: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: コラーゲン / 三重らせん / モデルペプチド / 高分解能解析 / 構造安定化 / 三重らせん構造 / 構造安定化機構 / 単結晶解析

Research Abstract

Understanding the mechanism of the stabilization and destabilization of a collagen helix induced by Hyp residues is one of important issues in structural study of collagen. The present study has revealed the rule for ring puckering of Hyp residues, which governs stabilization and destabilization of collagen helix, by the high resolution structure analyses of collagen-model peptides.

Research Products

• [Journal Article] コラーゲンの分子構造・高次構造 (2010)
  • Author(s): 奥山健二、川口辰也
  • Journal Title: 高分子論文集 67 Pages: 229-247
  • NAID: 10026213462
  • Peer Reviewed
• [Journal Article] Anomalous Dehydration Behavior of a Short Collagen Model Polypeptide, (L-Prolyl-L-ProlylGlycyl)5, in Aqueous Solution (2010)
  • Author(s): T. Shikata, N. Yoshida, K. Okuyama
  • Journal Title: J. Phys. Chem. Letters Pages: 412-416
  • Peer Reviewed
• [Journal Article] Bachinger, Structure analyses of two crystal modifications of (Pro-Pro-Gly)4-Hyp-Hyp-Gly-(Pro-Pro-Gly)4 revealed the puckering preference of Hyp(X) in the Hyp(X):Hyp(Y) and Hyp(X):Pro(Y) stacking pairs in collagen-helix (2010)
  • Author(s): K. Okuyama, T. Morimoto, H. Narita, T. Kawaguchi, K. Mizuno, H.P.
  • Journal Title: Protein Sci., Acta Cryst. D66 Pages: 88-96
  • Peer Reviewed
• [Journal Article] Two crystal modifications of (Pro-Pro-Gly)_4-Hyp-Hyp-Gly-(Pro-Pro-Gly)_4 reveal the puckering preference of Hyp(X) in the Hyp(X):Hyp(Y) and Hyp(X):Pro(Y) (2010)
  • Author(s): K.Okuyama
  • Journal Title: Acta Cryst. D66 Pages: 88-96
  • Peer Reviewed
• [Journal Article] Structure, Dynamics and Hydration of a Collagen Model Polypeptide, (L-Prolyl-L-ProlylGlycyl)10, in Aqueous Media: a Chemical Equilibrium Analysis of Triple Helix-to-Single Coil Transition (2009)
  • Author(s): T. Shikata, A. Minakawa, K. Okuyama
  • Journal Title: J. Phys. Chem. B. 113(43) Pages: 14504-14512
  • Peer Reviewed
• [Journal Article] Dynamics and Hydration Behavior of a Short Collagen Model Polypeptide, (L-Prolyl-L-ProlylGlycyl)5, in Aqueous Media (2009)
  • Author(s): T. Shikata, N. Yoshida, A. Minakawa, K. Okuyama
  • Journal Title: J. Phys. Chem. B. 113(27) Pages: 9055-9058
  • Peer Reviewed
• [Journal Article] High resolution structures of collagen-like peptides, (Pro-Pro-Gly)4-Xaa-Yaa-Gly-(Pro-Pro-Gly)4-Implications for triple-helix hydration and Hyp(X) puckering (2009)
  • Author(s): K. Okuyama, C. Hongo, G. Wu, K. Mizuno, K. Noguchi, S. Ebisuzaki, Y. Tanaka, N. Nishino, H.P. Bachinger
  • Journal Title: Biopolymers 91 Pages: 361-372
  • Peer Reviewed
• [Journal Article] High-resolution Structures of Collagen-like Peptides [(Pro-Pro-Gly)_4-Xaa-Yaa-Gly--(Pro-Pro-Gly)_4] : Implications for Triple-helix Hydration and Hyp(X) Puckering (2009)
  • Author(s): K.Okuyama
  • Journal Title: Biopolymers 91 Pages: 361-372
  • Peer Reviewed
• [Journal Article] Comment on Microfibrillar structure of type 1 collagen in situ by Orgel et al. (2006), Proc. Natl Acad. Sci. USA, 103, 9001-9005 (2009)
  • Author(s): K.Okuyama
  • Journal Title: Acta Cryst. D65 Pages: 1007-1008
  • Peer Reviewed
• [Journal Article] Chain dimension and hydration behavior of collagen model peptides in aqueous solution: [Glycyl-4(R)-Hydroxyprolyl-4(R)-Hydroxyproline]n and [Glycyl-Prolyl-4(R)-Hydroxyproline]n (2008)
  • Author(s): K. Terao, K. Mizuno, M. Murashima, Y. Kita, C. Hongo, K. Okuyama, T. Norisue, H.P. Bachinger
  • Journal Title: Macromolecules 41 Pages: 7203-7210
  • Peer Reviewed
• [Journal Article] Electrostatic interaction explains D-staggered structure of collagen (2008)
  • Author(s): T. Kawaguchi, K. Masakiyo, H. Jin, K. Okuyama
  • Journal Title: Acta Cryst. A64
  • Peer Reviewed
• [Journal Article] Structure of collagen-helix motif (2008)
  • Author(s): K. Okuyama
  • Journal Title: Acta Cryst. A64
  • Peer Reviewed
• [Journal Article] Crystal structure of human type III collagen G991-G1032 cystine knot containing peptide shows both 7/2 and 10/3 triple helical symmetries (2008)
  • Author(s): S.P. Boudko, J. Engel, K. Okuyama, K. Mizuno, H.P. Bachinger, M.A. Schumacher
  • Journal Title: J. Biochem. 283 Pages: 32580-32589
  • Peer Reviewed
• [Journal Article] Heat Treatment Strengthens Human Dentin (2008)
  • Author(s): M. Hayashi, E.V. Koychev, K. Okamura, A. Sugeta, C. Hongo, K. Okuyama, S. Ebisu
  • Journal Title: J. Dent. Res. 87(8) Pages: 762-766
  • Peer Reviewed
• [Journal Article] Revisiting the molecular structure of collagen (2008)
  • Author(s): K. Okuyama
  • Journal Title: Connect. Tissue Research 49 Pages: 1-12
  • Peer Reviewed
• [Journal Article] 7/2-Helical model for collagen-Evidence from native collagen and model peptides-, Proceeding of Recent Trends in Collagen, Diamond Jubilee Conference (2008)
  • Author(s): K. Okuyama
  • Journal Title: Central Leather Research Institute, Chennai, India Pages: 10-19
• [Journal Article] Crystal structure of human type III collagen G991-G1032 cystine knot containing peptide shows both 7/2 and 10/3 triple helical symmetries (2008)
  • Author(s): S. P. Boudko
  • Journal Title: J. Biol. Chem. 283 Pages: 32580-32589
  • Peer Reviewed
• [Journal Article] 7/2-helical model for collagen -Evidence from native collagen and model peptides- (2008)
  • Author(s): Okuyama, K.
  • Journal Title: Recent Trends in Collagen 2008 Pages: 10-19
• [Journal Article] Revisiting the molecular structure of collagen (2008)
  • Author(s): Okuyama, K.
  • Journal Title: Connect. Tissue Res. (In press)
  • Peer Reviewed
• [Journal Article] Unique side chain conformation of a Leu residue in a triple-helical structure (2007)
  • Author(s): K. Okuyama, H. Narita, T. Kawaguchi, K. Noguchi, Y. Tanaka, N. Nishino
  • Journal Title: Biopolymers 86 Pages: 212-221
  • Peer Reviewed
• [Journal Article] Unique side chain conformation of a Leu residue in a triple-helical structure (2007)
  • Author(s): Okuyama, K., et. al.
  • Journal Title: Biopolymers 36 Pages: 212-221
  • Peer Reviewed
• [Journal Article] Comments on Microfibrillar structure of type I collagen in situ by Orgel, et al. (2006)
  • Author(s): K. Okuyama, H.P. Bachinger, K. Mizuno, S. Boudko, J. Engel, R. Berisio, L. Vitagliano
  • Journal Title: PNAS, Acta Cryst. 103,D65
  • Peer Reviewed
• [Presentation] Stabilization mechanism by Hyp-Thr-Gly sequence in collagen-helix (2009)
  • Author(s): K. Miyama, T. Morimoto, K. Masakiyo, T. Kawaguchi, K. Okuyama, K. Mizuno, H.P. Bachinger
  • Organizer: AsCA'09 Joint Conference of the Asean Crystallographic Association and Chinese Crystallography Society
  • Place of Presentation: Beijing, China
  • Year and Date: 2009-10-24
• [Presentation] Stabilization mechanism by Hyp-Thr-Gly sequence in collagen-helix (2009)
  • Author(s): K.Miyama
  • Organizer: AsCA'09 Joint Conference of the Asian Crystallographic Association and Chinese Crystallography Society
  • Place of Presentation: 中国北京
  • Year and Date: 2009-10-24
• [Presentation] コラーゲンモデルペプチド(Pro-Pro-Gly)4- Hyp-Yaa-Gly-(Pro-Pro-Gly)4 (Yaa = Thr, Var, Ser)のX線結晶構造解析 (2009)
  • Author(s): 三山啓太、森本辰也、正清孝一、川口辰也、奥山健二、H.P. Bachinger
  • Organizer: 日本結晶学会年次大会
  • Place of Presentation: 関西学院大学
• [Presentation] コラーゲンモデルペプチド(Pro-Pro-Gly)4-Hyp-Thr-Gly-(Pro-Pro-Gly)4のX線結晶構造解析 (2008)
  • Author(s): 三山啓太、Nattha Jiravanichanun、奥山健二、水野一乗、Hans Peter Bachinger
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学、大阪
• [Presentation] 水溶液中における(Pro-Pro-Gly)10の三重らせんー1本鎖転移挙動 (2008)
  • Author(s): 喜田裕介、寺尾憲、奥山健二
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学、大阪
• [Presentation] 高分解能解析で分かったコラーゲンらせんにおける第1層の水と第2層の水 (2008)
  • Author(s): 奥山健二
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学、大阪
• [Presentation] Structure of collagen-helix motif, International Union of Crystallography (2008)
  • Author(s): K. Okuyama
  • Organizer: XXI Congress and General Assembly
  • Place of Presentation: Osaka, Japan
• [Presentation] Electrostatic interaction explains D-staggered structure of collagen, International Union of Crystallography (2008)
  • Author(s): T. Kawaguchi, K. Masakiyo, H. Jin, K. Okuyama
  • Organizer: XXI Congress and General Assembly
  • Place of Presentation: Osaka, Japan
• [Presentation] 小角X線によるアテロコラーゲンD-stagger構造の研究 (2008)
  • Author(s): 金海哲、川口辰也、服部俊治、奥山健二
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] 7/2らせん対称とアミノ酸配列に基づくコラーゲン繊維中のD-staggerの理解 (2008)
  • Author(s): 正清孝一、川口辰也、奥山健二
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] 7/2-helical model for collagen-Evidence from native collagen and model peptides-, Recent Trends in Collagen (2008)
  • Author(s): K. Okuyama
  • Organizer: Diamond Jubilee Conference, Central Leather Research Institute
  • Place of Presentation: Chennai, India
• [Presentation] Structure of collagen-helix motif (2008)
  • Author(s): K. Okuyama
  • Organizer: International Union of Crystallography, XXI Congress and General Assembly
  • Place of Presentation: 大阪国際会議場、大阪
• [Presentation] 7/2-helical model for collagen -Evidence from native collagen and model peptides- (2008)
  • Author(s): Okuyama, K.
  • Organizer: Recent Trend in Collagen, Diamond Jubilee Conference
  • Place of Presentation: Chennai, India
• [Presentation] コラーゲン繊維におけるD-stagger構造 (2007)
  • Author(s): 奥山健二、正清孝一、金海哲、川口辰也
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジューム
  • Place of Presentation: 千里ライフサイエンスセンター、豊中
• [Presentation] アテロコラーゲンの小角x線回折 (2007)
  • Author(s): 金海哲、川口辰也、奥山健二
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジューム
  • Place of Presentation: 千里ライフサイエンスセンター、豊中
• [Presentation] アテロコラーゲンの小角x線回折 (2007)
  • Author(s): 金海哲、奥山健二
  • Organizer: 日本結晶学会年会
  • Place of Presentation: 東京工業大学
• [Presentation] Stabilization of collagen triple-helix by Arg in the Y position (2007)
  • Author(s): T. Morimoto, M. Haga, C. Hongo, K. Noguchi, K. Okuyama, T. Tanaka
  • Organizer: The 8th Conference of the Asian Crystallographic Association (AsCA'07)
  • Place of Presentation: aipei, Taiwan
• [Presentation] Understanding of D-stagger in collagen fibers based on the 7/2-helical structure and amino acid sequence (2007)
  • Author(s): K. Masakiyo, T. Kawaguchi, K, Okuyama
  • Organizer: The 8th Conference of the Asian Crystallographic Association (AsCA'07)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] The structural analysis of the collagen-model peptide, (Pro-Pro-Gly)4- Hyp-Asp-Gly-(Pro-Pro-Gly)4 crystal (2007)
  • Author(s): T. Kawaguchi, M. Shimura, C. Hongo, K. Noguchi, K. Okuyama, K. Mizuno, H.P. Bachinger
  • Organizer: The 8th Conference of the Asian Crystallographic Association (AsCA'07)
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Understanding of D-stagger in collagen fibrils (2007)
  • Author(s): K. Okuyama
  • Organizer: 7th Pan Pacific Connective Tissue Societies Symposium, Shangri-La Resort
  • Place of Presentation: Cairns, Australia
• [Presentation] 静電相互作用に基づくコラーゲン高次構造におけるD-staggerの解読 (2007)
  • Author(s): 奥山健二
  • Organizer: 特定領域研究「生体超分子構造」第3回ワークショップ
  • Place of Presentation: ホテルニュウアカオ、熱海、静岡
• [Presentation] 放射光で分かったコラーゲンの分子構造と高次構造 (2007)
  • Author(s): 奥山健二
  • Organizer: 大阪大学蛋白質研究所セミナー「放射光が拓く繊維高分子のルネッサンス」、蛋白質研究所
  • Place of Presentation: 大阪大学、吹田
• [Presentation] コラーゲンモデルペプチド(Pro-Pro-Gly)4-Hyp-Asp-Gly-(Pro-Pro-Gly)4の結晶構造解析 (2007)
  • Author(s): 川口辰也、本郷千鶴、奥山健二、志村正紀、野口恵一、水野一乗、H.P. Bachinger
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Stabilization of collagen triple-helix by Arg in the Y position (2007)
  • Author(s): Okuyama, K.
  • Organizer: The 8th Conference of the Asian Crystallographic Association
  • Place of Presentation: Taipei, Taiwan
• [Presentation] The structure analysis of the collagen-model peptide, (Pro-Pro-Gly) 4-Hyp-Asp-Gly-(Pro-Pro-Gly) 4 crystal (2007)
  • Author(s): Kawaguchi, T.
  • Organizer: The 8th Conference of the Asian Crystallographic Association
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 溶液中におけるコラーゲンモデルペプチド三重らせん構造の熱安定性 (2007)
  • Author(s): 奥山健二
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 京都国際会議場、京都
• [Presentation] コラーゲンモデルペプチド(Pro-Pro-Gly)4-Hyp-Asp-Gly-(Pro-Pro-Gly)4の結晶構造解析 (2007)
  • Author(s): 川口辰也
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 京都国際会議場、京都
• [Book] コラーゲンの分子構造・高次構造、コラーゲンの製造と応用展開(谷原正夫監修) (2009)
  • Author(s): 奥山健二
  • Publisher: シーエムシー出版
• [Book] コラーゲン開発の現状と展望 (2009)
  • Author(s): 奥山健二(分担執筆)
  • Total Pages: 225
  • Publisher: シーエムシー出版