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Studies on biodiversity and phylogeny of soil protists, with special reference to soil flagellates.

Research Project

Project/Area Number 19370031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionHokkaido University

Principal Investigator

HORIGUCHI Takeo  Hokkaido University, 大学院・理学研究院, 教授 (20212201)

Research Collaborator OHUCHI Mariko  北海道大学, 大学院・理学院・大学院生修士課程
HANAFUSA Yukari  北海道大学, 大学院・理学院・大学院生修士課程
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywords土壌性鞭毛虫類 / 原生生物 / 多様性 / 分子系統解析 / 土壌性鞭毛虫 / 微細構造 / 大黒島
Research Abstract

The flagellates are an assemblage of unicellular organisms with flagella. Our knowledge on the flagellates inhabiting soil habitats in Japan was rather poor. We aimed to investigate biodiversity and phylogenetic affinities of Japanese soil flagellates. We have established over 180 culture strains and studied them with light and electron microscopy. We also investigated phylogenetic relationships by molecular phylogeny. As a result, we identified 24 genera (>50 spp., but exact species number is still to be determined) representing 5 protistan phyla. We also demonstrated that extensive diversification of Paraphysomonas took place in soil, independent of marine or freshwater habitats.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2010 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (9 results)

  • [Journal Article] Discovery of a novel type of body scale in the marine dinoflagellate, Amphidinium cupulatisquama sp. nov. (Dinophyceae).2009

    • Author(s)
      Tamura, M., Y. Takano, T. Horiguchi
    • Journal Title

      Phycol. Res. 57

      Pages: 304-312

    • NAID

      10027506127

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of a novel type of body scale in the marine dinoflagellate, Amphidinium cupulatisquama sp.nov.(Dinophyceae).2009

    • Author(s)
      Maiko Tamura
    • Journal Title

      Phycological Research 57

      Pages: 304-312

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of a novel type of body scale in the marine dinoflagellate, Amphidinium cupulatisquama sp. nov. (Dinophyceae)2009

    • Author(s)
      M. Tamura
    • Journal Title

      Phycological Research 57(In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Culture of the heterotrophic dinoflagellte Protoperidinium crassipes (Dinophyceae) with noncellular food items.2008

    • Author(s)
      Yamaguchi, A., T. Horiguchi
    • Journal Title

      J. Phycol. 44(4)

      Pages: 1090-1092

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Haramonas pauciplastida sp. nov. (Raphidophyceae, Heterokontophyta) and phylogenetic analysis of Haramonas species using SSU rDNA.2008

    • Author(s)
      H. Yamaguchi, M. Hoppenrath, K. Takishita, T. Horiguchi
    • Journal Title

      Phycol. Res. 56

      Pages: 127-138

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serial replacement of diatom endosymbionts in two freshwater dinoflagellates, Peridiniopsis spp. (Peridiniales, Dinophyceae)2008

    • Author(s)
      Y. Takano, G. Hansen, D. Fujita, T. Horiguchi
    • Journal Title

      Phycologia 47

      Pages: 41-53

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Culture of the heterotrophic dinoflagellate Protoperidinium crassipes (Dinophyceae) with noncellular food items2008

    • Author(s)
      A. Yamaguchi
    • Journal Title

      Journal of Phycology 44

      Pages: 1090-1092

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soil protists and soil animals on Daikokujima.2008

    • Author(s)
      S. Shimano
    • Journal Title

      Proceedings of the International Symposium "The Origin and Evolution of Natural Diversity."

      Pages: 151-152

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Haramonas pauciplastida sp. nov. (Raphidophyceae, Heterokontophyta) and phylogenetic analysis of Haramonas species using SSU rDNA.2008

    • Author(s)
      H. Yamaguchi
    • Journal Title

      Phycological Research 56(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soli protists and soil animals on Daikokujima.2007

    • Author(s)
      S. Shimano, T. Horiguchi
    • Journal Title

      Proceedings of the International Symposium "The Origin and Evolution of Natural Diversity". 1-5 October 2007, Sapporo, Japan.

      Pages: 151-152

    • Related Report
      2009 Final Research Report
  • [Presentation] 土壌性のParaphysomonas属(黄金色藻綱)と渦鞭毛藻類の分類学的研究2010

    • Author(s)
      花房友香里
    • Organizer
      日本藻類学会第34会大会
    • Place of Presentation
      筑波大学,つくば市
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 土壌性のParaphysomonas属(黄金色藻綱)と渦鞭毛藻類の分類学的研究。2010

    • Author(s)
      花房友香里、高野義人、堀口健雄
    • Organizer
      日本藻類学会第34回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2010-03-20
    • Related Report
      2009 Final Research Report
  • [Presentation] 土壌に生息する従属栄養性黄金色藻類の分類学的研究。2009

    • Author(s)
      花房友香里・堀口健雄
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-19
    • Related Report
      2009 Final Research Report
  • [Presentation] 土壌に生息する従属栄養性黄金色藻類の分類学的研究2009

    • Author(s)
      花房友香里
    • Organizer
      日本植物学会
    • Place of Presentation
      山形大学,山形市
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Diversity of soil flagellates in Japan.2009

    • Author(s)
      Hanafusa Y, Ohuchi M, Horiguchi T
    • Organizer
      9th International Phycological Congress
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-08-06
    • Related Report
      2009 Final Research Report
  • [Presentation] Diversity of soil flagellates in Japan.2009

    • Author(s)
      Yukari Hanafusa
    • Organizer
      9^<th> International Phycological Congress(第9回国際藻類学会議)
    • Place of Presentation
      オリンピック記念青少年総合センター,東京
    • Year and Date
      2009-08-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 土壌性鞭毛虫類の多様性。2009

    • Author(s)
      花房友香里・堀口健雄
    • Organizer
      日本藻類学第33回大会
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] 土壌性鞭毛虫類の多様性2009

    • Author(s)
      花房友香里
    • Organizer
      第33回日本藻類学会
    • Place of Presentation
      琉球大学(沖縄県)
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Soil protists and soil animals on Daikokujima.2007

    • Author(s)
      S. Shimano
    • Organizer
      International Symposium "The Origin and Evolution of Natural Diversity"
    • Place of Presentation
      札幌
    • Year and Date
      2007-10-03
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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