Clarification of controlling mechanism by proteins in soybean under flooding stress

• Project/Area Number: 19380015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: National Agricultural Research Organization
• Principal Investigator: KOMATSU Setsuko National Agricultural Research Organization, 作物研究所大豆生理研究チーム, チーム長 (90355751)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: ダイズ / 根系 / 湿害 / ネットワーク / プロテオーム

Research Abstract

Flooding is a major problem for soybean because it reduces the growth and grain yield. To investigate the function of proteins in the response to flooding stress, proteome techniques have been used. Proteins in cell wall, plasma membrane and mitochondria were remarkably up- or down-regulated under flooding stress. Especially, it is suggested that the improvement of the decrease of the lignification and energy metabolism might be important.

Research Products

• [Journal Article] Quantitative proteomic analyses of crop seedling sybjected to stress conditions ; a commentary. (2011)
  • Author(s): Nanjo Y, Nouri MZ, Komatsu S
  • Journal Title: Phytochemistry 72 Pages: 1263-1272
  • Peer Reviewed
• [Journal Article] Comparative proteomic analysis of early-stage soybean seedlings responses to flooding by using gel and gel-free techniques. (2010)
  • Author(s): Nanjo Y, Skulty L, Ashraf Y, Komatsu S
  • Journal Title: Journal of Proteome Research 9 Pages: 3989-4002
  • Peer Reviewed
• [Journal Article] Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches. (2010)
  • Author(s): Kong F, Oyanagi A, Komatsu S
  • Journal Title: Biochima et Biophysica Acta-Proteins and Proteomics 1804 Pages: 124-136
  • Peer Reviewed
• [Journal Article] Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress. (2010)
  • Author(s): Komatsu S, Kobayashi Y, Nishizawa K, Nanjo Y, Furukawa K
  • Journal Title: Amino Acids 39 Pages: 1435-1449
  • Peer Reviewed
• [Journal Article] Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches. (2010)
  • Author(s): Kong F, Oyanagi A, Komatsu S
  • Journal Title: Biochima et Biophysica Acta-Proteins and Proteomics Volume: 1804 Pages: 124-136
  • Peer Reviewed
• [Journal Article] Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress. (2010)
  • Author(s): Komatsu S, Kobayashi Y, Nishizawa K, Nanjo Y, Furukawa K
  • Journal Title: Amino Acids Volume: 39 Pages: 1435-1449
  • Peer Reviewed
• [Journal Article] Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches (2010)
  • Author(s): Kong F, Oyanagi A, Komatsu S
  • Journal Title: Biochima et Biophysica Acta-Proteins and Proteomics 1804 Pages: 124-136
  • Peer Reviewed
• [Journal Article] Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress (2010)
  • Author(s): Komatsu S, Kobayashi Y, Nishizawa K, Nanjo Y, Furukawa K
  • Journal Title: Amino Acids
  • Peer Reviewed
• [Journal Article] Analysis of plasma membrane proteome in soybean and application to flooding stress response. (2009)
  • Author(s): Komatsu S, Wada T, Abelea Y, Nouri Z, Nanjo Y, Nakayama N, Shimamura S, Yamamoto R, Nakamura T, Furukawa K
  • Journal Title: Journal of Proteome Research 8 Pages: 4487-4499
  • Peer Reviewed
• [Journal Article] Analysis of plasma membrane proteome in soybean and application to flooding stress response (2009)
  • Author(s): Komatsu S, Wada T, Abelea Y, Nouri Z, Nanjo Y, Nakayama N, Shimamura S, Yamamoto R, Nakamura T, Furukawa K
  • Journal Title: Journal of Proteome Research 8 Pages: 4487-4499
  • Peer Reviewed
• [Journal Article] Analysis of plasma membrane proteome in soybean and application to flooding stress response. (2009)
  • Author(s): Komatsu S, et al.
  • Journal Title: Journal of Proteome Research 8 Pages: 4487-4499
  • Peer Reviewed
• [Journal Article] Cytosolic ascorbate peroxidase 2 (cAPX) is involved in the soybean response to flooding. (2008)
  • Author(s): Shi F, Yamamoto R, Shimamura S, Hiraga S, Nakayama N, Nakamura T, Yukawa K, Hachinohe M, Matsumoto H, Komatsu S
  • Journal Title: Phytochemistry 69 Pages: 1295-1303
  • Peer Reviewed
• [Journal Article] Cytosolic ascorbate peroxidase 2 (cAPX) is involved in the soybean response to flooding (2008)
  • Author(s): Shi F, Yamamoto R, Shimamura S, Hiraga S, Nakayama N, Nakamura T, Yukawa K, Hachinohe M, Matsumoto H, Komatsu S
  • Journal Title: Phytochemistry 69 Pages: 1295-1303
  • Peer Reviewed
• [Journal Article] Ascorbate peroxidase 2 (Apx 2)involves in the response to submergence in soybean (2008)
  • Author(s): Fang Shi(第一著者)
  • Journal Title: Phytochemistry 69 Pages: 1295-1303
  • Peer Reviewed
• [Journal Article] Crop Proteomics:Aim at sustainable agriculture of tomorrow. (2007)
  • Author(s): G.Salekdeh, S.Komatsu
  • Journal Title: Proteomics 7 Pages: 2976-2996
  • Peer Reviewed
• [Presentation] 出芽期のダイズ冠水ストレスにより変動するミトコンドリアタンパク質のプロテオーム解析 (2010)
  • Author(s): 山本明史、西澤けいと、南條洋平、古川清、小松節子
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Soybean Proteome Database : its application to analysis of proteins under flooding stress. (2010)
  • Author(s): 小松節子
  • Organizer: The 3rd National Plant Proeomics Conference of China
  • Place of Presentation: Wuhan
  • Year and Date: 2010-05-24
• [Presentation] Proteome analysis of mitochondria proteins in soybean under flooding stress. (2010)
  • Author(s): Yamamoto, et al.
  • Organizer: 2nd International Symposium "Frontier in Agriculture Proteome Research"
  • Place of Presentation: つくば・農林交流研究センター
• [Presentation] 冠水処理により変動するダイズ細胞壁タンパク質群の解析 (2009)
  • Author(s): 小林之人、西澤けいと、南條洋平、島村聡、中村卓司、古川清、小松節子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 冠水処理により変動するダイズ細胞壁タンパク質群の解析 (2009)
  • Author(s): 小林之人, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ・横浜
  • Year and Date: 2009-12-10
• [Presentation] ダイズ細胞膜のプロテオーム解析 (2008)
  • Author(s): 和田拓也、NOURI Zaman、古川清、小松節子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Identification of osmotic stress related proteins in soybean plasma membrane. (2008)
  • Author(s): NOURI Zaman、小松節子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Identification of osmotic stress related proteins in soybean plasma membrane (2008)
  • Author(s): NOURI Zaman, 小松節子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] ダイズ細胞膜のプロテオーム解析 (2008)
  • Author(s): 和田拓也, Zaman Nouri, 古川清, 小松節子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2008-12-12
• [Presentation] Identification of osmotic stress related proteins in soybean plasma membrane (2008)
  • Author(s): Zaman Nouri, 小松節子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2008-12-12
• [Presentation] Identification of ascorbate peroxidase 2 in soybean root under flooding stress. (2008)
  • Author(s): Yukawa K, Komatsu S
  • Organizer: International Symposium of Plant Proteome Research: Contribution of Proteomics Technology in Creation of Useful Plants.
  • Place of Presentation: Tsukuba
• [Presentation] Identification of ascorbate peroxidase 2 in soybean root under flooding stress (2008)
  • Author(s): Yukawa K, Komatsu S
  • Organizer: International Symposium of Plant Proteome Research : Contribution of Proteomics Technology in Creation of Useful Plants
  • Place of Presentation: Tsukuba
• [Presentation] Identification of ascorbate peroxidase2 in soybean root under flooding stress through proteomics (2008)
  • Author(s): Kiyoshi Yukawa(第一著者)
  • Organizer: Frontier in Plant Proteome Research:Contribution of proteomics technology in creation of useful plants
  • Place of Presentation: Tsukuba
• [Presentation] Ascorbate peroxidase 2 (cAPX) is involved in the soybean response to flooding. (2007)
  • Author(s): Shi F, Yamamoto R, Shimamura S, Hiraga S, Nakamura N, Nakamura T, Yukawa K, Hachinohe M, Matsumoto H, Komatsu S
  • Organizer: 9th Conference of the International Society for Plant Anaerobiosis.
  • Place of Presentation: Sendai
• [Presentation] Ascorbate peroxidase 2 (cAPX) is involved in the soybean response to flooding (2007)
  • Author(s): Shi F, Yamamoto R, Shimamura S, Hiraga S, Nakamura N, Nakamura T, Yukawa K, Hachinohe M, Matsumoto H, Komatsu S
  • Organizer: 9^<th> Conference of the International Society for Plant Anaerobiosis
  • Place of Presentation: Sendai
• [Presentation] Ascorbate peroxidase2(Apx 2)is involved in the response to flooding in soybean. (2007)
  • Author(s): Fang Shi(第一著者)
  • Organizer: 9th Conference of the Interrnational Society for Plant Anaerobiosis.
  • Place of Presentation: Sendai