Molecular basis of G-protein mediated PAMPs signaling

• Project/Area Number: 19380028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KAWASAKI Tsutomu Nara Institute of Science and Technology, バイオサイエンス研究科, 准教授 (90283936)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 植物免疫 / Gタンパク質 / 耐病性 / PAMPs / 信号伝達 / GEF

Research Abstract

Plants induce a series of immune responses through recognition of pathogen-derived molecules (Pathogen-associated molecular patterns ; PAMPs). We analyzed the molecular mechanism of activation of Rac/Rop GTPase during PAMPs triggered responses, and found that Rac/Rop GTPase is regulated through activation of Rac/Rop GEF by PAMPs receptor

Research Products

• [Journal Article] Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice (2010)
  • Author(s): Fujiwara, T., Maisonneuve, S., Isshiki, M., Mizutani, M., Chen, L., Wong, H.L., Kawasaki, T., Shimamoto, K.
  • Journal Title: J. Biol. Chem. 285 Pages: 11308-11313
  • Peer Reviewed
• [Journal Article] Hop/Sti1 and HSP90 are involved in maturation and transport of a PAMP receptor in rice innate immunity (2010)
  • Author(s): Chen, L., Hamada, S., Fujiwara, M., Zhu, T., Thao, N.P., Wong, H.L., Krishna, P., Ueda, T., Kaku, H., Shibuya, N., Kawasaki, T., Shimamoto, K.
  • Journal Title: Cell Host Microbe 7 Pages: 185-196
  • NAID: 130006991976
  • Peer Reviewed
• [Journal Article] Analysis of the Rac/Rop Small GTPase Family in Rice: Expression, Subcellular Localization and Role in Disease Resistance (2010)
  • Author(s): Chen, L., Shiotani, K., Togashi, T., Miki, D., Aoyama, M., Wong, H.L., Kawasaki, T., Shimamoto, K
  • Journal Title: Plant Cell. Physiol. 51 Pages: 585-595
  • NAID: 10027348474
  • Peer Reviewed
• [Journal Article] The structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications (2010)
  • Author(s): Oda, T., Hashimoto, H., Kuwabara, N., Akashi, S., Hayashi, K., Kojima, C., Wong, H.L., Kawasaki, T., Shimamoto, K., Sato, M., Shimizu, T.
  • Journal Title: J. Biol. Chem. 285 Pages: 1435-1445
  • Peer Reviewed
• [Journal Article] Sekiguchi lesion encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice (2010)
  • Author(s): T.Fujiwara
  • Journal Title: J.Biol.Chem (印刷中)
  • Peer Reviewed
• [Journal Article] Hop/Stil and HSP90 are involved in maturation and transport of a PAMP receptor in rice innate immunity (2010)
  • Author(s): L.Chen
  • Journal Title: Cell Host Microbe (印刷中)
  • Peer Reviewed
• [Journal Article] Analysis of the Rac/Rop Small GTPase Family in Rice : Expression, Suhcellular Localization and Role in Disease Resistance (2010)
  • Author(s): L.Chen
  • Journal Title: Plant Cell Physiol (印刷中)
  • Peer Reviewed
• [Journal Article] Analysis of the Rac/Rop Small GTPase Family in Rice : Expression, Subcellular Localization and Role in Disease Resistance (2010)
  • Author(s): L.Chen
  • Journal Title: Plant Cell Physiol (印刷中)
  • Peer Reviewed
• [Journal Article] Proteome analysis of detergent resistant membranes (DRMs) associated with OsRac1 mediated innate immunity in rice. (2009)
  • Author(s): Fujiwara, M., Hamada, S., Hiratsuka, M., Fukao, Y., Kawasaki, T., Shimamoto, K.
  • Journal Title: Plant Cell. Physiol. 50 Pages: 1191-1200
  • NAID: 10027341177
  • Peer Reviewed
• [Journal Article] The structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications (2009)
  • Author(s): T.Oda
  • Journal Title: J Biol Chem 285 Pages: 1435-1445
  • Peer Reviewed
• [Journal Article] Proteome analysis of detergent resistant membranes (DRMs) associated with OsRac1 mediated innate immunity in rice (2009)
  • Author(s): M.Fujiwara
  • Journal Title: Plant Cell Physiol 50 Pages: 1191-1200
  • Peer Reviewed
• [Journal Article] Proteome analysis of detergent resistant membranes(DRMs)associated with OsRacl mediated innate immunity in rice (2009)
  • Author(s): M.Fujiwara
  • Journal Title: Plant Cell Physiol 50 Pages: 1191-1200
  • Peer Reviewed
• [Journal Article] Crystallographic characterization of the N-terminal domain of plant NADPH oxidase. Acta. Crystallogr. Sect. F. Struct. (2008)
  • Author(s): Oda, T., Hashimoto, H., Kuwabara, N., Hayashi, K., Kojima, C., Kawasaki, T., Shimamoto, K., Sato, M., Shimizu, T.
  • Journal Title: Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 64 Pages: 867-869
  • Peer Reviewed
• [Journal Article] RACK1 functions in rice innate immunity by interacting with the Rac1 immune complex. (2008)
  • Author(s): Nakashima, A., Chen, L., Thao, N.P., Fujiwara, M., Wong, H.L., Kuwano, M., Umemura, K., Shirasu, K., Kawasaki, T., Shimamoto, K.
  • Journal Title: Plant Cell 20 Pages: 2265-2279
  • Peer Reviewed
• [Journal Article] Constitutive activation of a CC-NB-LRR protein alters morphogenesis through the cytokinin pathway. (2008)
  • Author(s): Igari, K., Endo, S., Hibara, K., Aida, M., Sakakibara, H., Kawasaki, T., Tasaka, M.
  • Journal Title: Plant J. 55 Pages: 14-27
  • Peer Reviewed
• [Journal Article] Crystallographic characterization of the N-terminal domain of plant NADPH oxidase (2008)
  • Author(s): T.Oda
  • Journal Title: Acta.Crystallogr F64 Pages: 867-869
  • Peer Reviewed
• [Journal Article] RACK1 functions in rice innate immunity by interacting with the Raclimmune complex (2008)
  • Author(s): A.Nakashima
  • Journal Title: Plant Cell 20 Pages: 2265-2279
  • Peer Reviewed
• [Journal Article] Constitutive activation of a CC-NB-LRR protein alters morphogenesis through the cytokinin pathway (2008)
  • Author(s): K.Igari
  • Journal Title: Plant J 55 Pages: 14-27
  • Peer Reviewed
• [Journal Article] Constitutive activation of a CC-NB-LRR protein alters morphogenesis through the cytokinin pathway. (2008)
  • Author(s): K. Igari
  • Journal Title: Plant J
  • Peer Reviewed
• [Journal Article] Rice guanine nucleotide exchange factors for small GTPase OsRac1 involved in innate immunity of rice (2008)
  • Author(s): T. Kawasaki
  • Journal Title: Advances in Genetics, Genomics and Control of Rice Blast Disease
• [Journal Article] Rice Ptila negatively regulates RAR1 dependent defense responses (2007)
  • Author(s): A. Takahashi
  • Journal Title: Plant Cell 19 Pages: 2940-2951
  • Peer Reviewed
• [Journal Article] RAR1 and HSP90 form a complex with Rac/Rop GTPase and function in innate immune responses in rice (2007)
  • Author(s): N. P. Thao
  • Journal Title: Plant Cell 19 Pages: 4035-4045
  • Peer Reviewed
• [Journal Article] Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. (2007)
  • Author(s): H. L. Wong
  • Journal Title: Plant Cell 19 Pages: 4022-4034
  • Peer Reviewed
• [Presentation] Analysis of PAMPs-induced OsRac1 activation using FRET biosensor in rice (2009)
  • Author(s): 赤松明、奥田淳、川崎努、Wong Hann Ling、島本功
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Rタンパク質によるGタンパク質OsRac1の活性化が植物免疫に重要である (2009)
  • Author(s): 河野洋治、赤松明、林敬子、宝泉雄介、奥田淳、中島綾子、高橋弘喜、吉田均、Hann Ling Wong、川崎努、島本功
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Rタンパク質によるGタンパク質OsRaclの活性化が植物免疫に重要である (2009)
  • Author(s): 河野洋治
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Analysis of PAMPs-induced OsRacl activation using FRET biosens or in rice (2009)
  • Author(s): 赤松明
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] FRETバイオセンサーを用いたPAMPによるOsRac1の活性化解析 (2009)
  • Author(s): 赤松明、奥田淳、川崎努、Hann Ling Wong、島本功
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 植物免疫信号伝達系におけるセロトニンの機能解析 (2009)
  • Author(s): 藤原幹、Sylvie Maisonneuve、一色正之、水谷正治、川崎努、島本功
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Rタンパク質によるGタンパク質OsRac1の活性化が植物免疫に重要である (2009)
  • Author(s): 河野洋治、赤松明、林敬子、宝泉雄介、中島綾子、高橋弘喜、吉田均、Hann Ling Wong、川崎努、島本功
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 植物免疫を制御するOsRac1 複合体(Defensome)の解析 (2009)
  • Author(s): 濱田聡
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Rタンパク質によるGタンパク質OsRaclの活性化が植物免疫に重要である (2009)
  • Author(s): 河野洋治
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 植物免疫を制御するOsRacl複合体(Defensome)の解析 (2009)
  • Author(s): 濱田聡
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] FRETバイオセンサーを用いたPAMPによるOsRaclの活性化解析 (2009)
  • Author(s): 赤松明
  • Organizer: 第82回日本生化学学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Type III effectors of Xanthomonas oryza pv. oryzae suppress PAMPs- triggered immunity in rice (2009)
  • Author(s): Yamaguchi, K., Furutani, A., Ochiai, H., Tsuge, S., Shimamoto, K., Kawasaki, T.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ, ケベック
  • Year and Date: 2009-07-20
• [Presentation] Identification of DH-PH type GDP-GTP exchange factor for Rac/Rop GTPase in plant immune response (2009)
  • Author(s): Kawasaki, T., Yamaguchi, K., Shimamoto, K.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ, ケベック
  • Year and Date: 2009-07-20
• [Presentation] LAP (bHLH) is a transcription factor activated by OsRac1 in rice innate immunity (2009)
  • Author(s): Kim, S., Oikawa, T., Kyozuka, J., Wong, H.L., Kawasaki, T., Shimamoto, K.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ, ケベック
  • Year and Date: 2009-07-20
• [Presentation] Activation of RacGTPase-mediated defence signalling by pathogen- associated molecular patterns and guanine nucleotide exchange factor in rice (2009)
  • Author(s): Wong, H.L., Akamatsu, A., Imai, K., Okuda, J., Zhu, T., Kawasaki, T., Shimamoto, K.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ, ケベック
  • Year and Date: 2009-07-20
• [Presentation] Analysis of PAMPs-induced OsRac1 activation using FRET biosensor in rice (2009)
  • Author(s): Akamatsu, A., Okuda, J., Kawasaki, T., Wong, H.L., Shimamoto, K.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ, ケベック
  • Year and Date: 2009-07-20
• [Presentation] Analysis of PAMPs-induced OsRac1 activation using FRET biosensor in rice (2009)
  • Author(s): Akamatsu, A
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ・ケベック
  • Year and Date: 2009-07-20
• [Presentation] Activation of RacGTPase-mediated defence signalling by pathogen-associated molecular patterns and guanine nucleotide exchange factor in rice (2009)
  • Author(s): Wong, H.L.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ・ケベック
  • Year and Date: 2009-07-20
• [Presentation] LAP (bHLH) is a transcription factor activated by OsRac1 in rice innate immunity (2009)
  • Author(s): Kim, S.H.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ・ケベック
  • Year and Date: 2009-07-20
• [Presentation] Identification of DH-PH type GDP-GTP exchange factor for Rac/Rop GTPase in plant immune response (2009)
  • Author(s): Kawasaki, T
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ・ケベック
  • Year and Date: 2009-07-20
• [Presentation] Type III effectors of Xanthomonas oryza pv. oryzae suppress PAMPs-triggered immunity in rice (2009)
  • Author(s): Yamaguchi, K.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ・ケベック
  • Year and Date: 2009-07-20
• [Presentation] Analysis of PAMPs-induced OsRacl activation using FRET biosens or in rice (2009)
  • Author(s): 赤松明
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ
  • Year and Date: 2009-07-20
• [Presentation] Activation of RacGTPase-mediated defence signalling by pathogen- associated molecular patterns and guanine nucleotide exchange factor in rice (2009)
  • Author(s): Wong, H.L.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ
  • Year and Date: 2009-07-20
• [Presentation] LAP(bHLH)is a transcription factor activated by OsRacl in rice innate immunity (2009)
  • Author(s): Kim, S.H.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ
  • Year and Date: 2009-07-20
• [Presentation] Identification of DH-PH type GDP-GTP exchange factor for Rac/Rop GTPase in plant immune response (2009)
  • Author(s): Kawasaki, T
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ
  • Year and Date: 2009-07-20
• [Presentation] Type III effectors of Xanthomonas oryza pv.oryzae suppress PAMPs- triggered immunity in rice (2009)
  • Author(s): 山口公志
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: カナダ
  • Year and Date: 2009-07-20
• [Presentation] イネ白葉枯病菌TypeIIIエフェクターの機能解析 (2009)
  • Author(s): 山口公志、古谷綾子、落合弘和、津下誠治、島本功、川崎努
  • Organizer: 日本植物病理学会
  • Place of Presentation: 山形
  • Year and Date: 2009-03-27
• [Presentation] イネ白葉枯病菌 Type IIIエフェクターの機能解析 (2009)
  • Author(s): 山口公志
  • Organizer: 日本植物病理学会
  • Place of Presentation: 山形
  • Year and Date: 2009-03-27
• [Presentation] 植物免疫信号伝達系におけるセロトニンの機能解析 (2009)
  • Author(s): 藤原幹、Sylvie Maisonneuve、一色正之、水谷正治、川崎努、島本功
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] 低分子量GTP結合タンパク質イネOsRac1を介した抵抗性タンパク質による過敏感反応死と耐病性の制御機構 (2009)
  • Author(s): 河野洋治、林敬子、赤松明、宝泉雄介、Loh, P.C.、中島綾子、高橋弘喜、吉田均、川崎努、島本功
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] 低分子量GTP結合タンパク質イネOsRaclを介した抵抗性タンパク質による過敏感反応死と耐病性の制御機構 (2009)
  • Author(s): 河野洋治
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] 低分子量GTP結合タンパク質イネOsRaclを介した抵抗性タンパク質による過敏感反応死と耐病性の制御機構 (2008)
  • Author(s): 河野洋治
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 植物免疫信号伝達系におけるセロトニンの機能解析 (2008)
  • Author(s): 藤原幹、Maisonneuve, S.、一色正之、水谷正治、川崎努、島本功
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] FRETバイオセンサーを用いた植物細胞における低分子量Gタンパク質の解析 (2008)
  • Author(s): 赤松明、河野洋治、奥田淳、Wong, H.L.、川崎努、島本功
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 低分子量GTP結合タンパク質イネOsRac1を介した抵抗性タンパク質による過敏感反応死と耐病性の制御機構 (2008)
  • Author(s): 河野洋治、Loh, P.C.、林敬子、赤松明、中島綾子、高橋弘喜、吉田均、川崎努、島本功
  • Organizer: 31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] LAP (bHLH) is a transcription factor activated by OsRac1 in rice innate immunity (2008)
  • Author(s): Kim, S.H., Oikawa, T., Kyozuka, J., Wong, H.L., Kawasaki, T., Shimamoto, K.
  • Organizer: The 6th International symposium on rice functional genomics
  • Place of Presentation: 韓国, 済州
  • Year and Date: 2008-11-10
• [Presentation] LAP(bHLH)is a transcription factor activated by OsRacl in rice innate immunity (2008)
  • Author(s): S-H.Kim
  • Organizer: The 6th International symposium on rice functional genomics
  • Place of Presentation: 韓国
  • Year and Date: 2008-11-10
• [Presentation] RACK1 functions in rice innate immunity by interacting with the OsRac1 immune complex (2008)
  • Author(s): Chen, L., Nakashima, A., Thao, N.P., Fujiwara, M., Wong, H.L., Kawasaki, T., Shimamoto, K
  • Organizer: Plant Genomics in China
  • Place of Presentation: 中国, 広州
  • Year and Date: 2008-07-18
• [Presentation] RACK1 functions in rice innate immunity by interacting with the OsRacl immune complex (2008)
  • Author(s): L.Chen
  • Organizer: Plant Genomics in China
  • Place of Presentation: 中国
  • Year and Date: 2008-07-18
• [Presentation] イネの病害抵抗性に関わるOsRac1 GEFの同定 (2007)
  • Author(s): 西出圭太
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] FRETバイオセンサーを用いた植物細胞における低分子量Gタンパク質の活性解析 (2007)
  • Author(s): 奥田淳
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] RacGEFs function as activators of small GTPase OsRac1 in innate immunity of rice (2007)
  • Author(s): T. Kawasaki
  • Organizer: 4th international rice blast conference
  • Place of Presentation: 中国
  • Year and Date: 2007-10-10
• [Presentation] Rac GEFs function as activators of small GTPase OsRacl in innate immunity of rice (2007)
  • Author(s): T. Kawasaki
  • Organizer: XIII International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: イタリア
  • Year and Date: 2007-07-23
• [Presentation] Rac GTPase-mediated innate immunity in rice (2007)
  • Author(s): K. Shimamoto
  • Organizer: XIII International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: イタリア
  • Year and Date: 2007-07-23
• [Presentation] Stil/Hop, a HSP90 cochaperon is a novel component in defense signaling in rice (2007)
  • Author(s): L. Chen
  • Organizer: XIII International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: イタリア
  • Year and Date: 2007-07-23
• [Presentation] Rac GTPase regulated reactive oxygen production by binding to the N-terminal extension of NADPH oxidase in rice (2007)
  • Author(s): H. Wong
  • Organizer: XIII International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: イタリア
  • Year and Date: 2007-07-23