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Studies on biosynthesis, metabolism and signal transduction of brassinosteroids

Research Project

Project/Area Number 19380069
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

FUJIOKA Shozo  The Institute of Physical and Chemical Research, 中野生体膜研究室, 副主任研究員 (60165355)

Co-Investigator(Kenkyū-buntansha) 瀬戸 秀春  独立行政法人理化学研究所, 辻本細胞生化学研究室, 専任研究員 (40175419)
嶋田 幸久  独立行政法人理化学研究所, ゲノム機能統合化研究チーム, 上級研究員 (30300875)
Co-Investigator(Renkei-kenkyūsha) SETO Hideharu  独立行政法人理化学研究所, 中野生体膜研究室, 専任研究員 (40175419)
SHIMADA Yukihisa  独立行政法人理化学研究所, ゲノム機能統合化研究, チームチームリーダー (30300875)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywordsブラシノステロイド / 生合成 / 代謝 / シロイヌナズナ / イネ / 転写因子 / シグナル伝達 / 生合成調節
Research Abstract

This study established a novel pathway via early C-2 hydroxylation in brassinosteroid (BR) biosynthesis as well as new functions of UGT73C6 and rice CYP734As in BR catabolism. ASKθoverexpressors displayed a bri1-like phenotype, and accumulated high levels of BRs. ASKθwas shown to function as a negative regulator in BR signaling. Through the analysis of BR-related mutants such as tcp1-1D and ces-D, we identified TCP1 and CESTA as transcription factors that positively regulate BR biosynthesis.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (53 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (31 results) (of which Peer Reviewed: 31 results) Presentation (22 results)

  • [Journal Article] Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana.2011

    • Author(s)
      Sigrid Husar, Franz Berthiller, Shozo Fujioka, Wilfried Rozhon, Mamoona Khan, Florian Kalaivanan, Luisa Elias, Gillian S. Higgins, Yi Li, Rainer Schuhmacher, Rudolf Krska, Hideharu Seto, Fabian E. Vaistij, Dianna Bowles, Brigitte Poppenberger
    • Journal Title

      BMC Plant Biology 11

      Pages: 51-51

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] CESTA, a positive regulator of brassinosteroid biosynthesis.2011

    • Author(s)
      Brigitte Poppenberger, Wilfried Rozhon, Mamoona Khan, Sigrid Husar, Gerhard Adam, Christian Luschnig, Shozo Fujioka, Tobias Sieberer
    • Journal Title

      EMBO J. 30

      Pages: 1149-1161

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intragenic suppression of a trafficking-defective brassinosteroid receptor mutant in Arabidopsis.2010

    • Author(s)
      Youssef Belkhadir, Amanda Durbak, Michael Wierzba, Robert J. Schmitz, Andrea Aguirre, Rene Michel, Scott Rowe, Shozo Fujioka, Frans E.Tax
    • Journal Title

      Genetics 185

      Pages: 1283-1296

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] RAV-Like1 maintains brassinosteroid homeostasis via the coordinated activation of BRI1 and biosynthetic genes in rice.2010

    • Author(s)
      Byoung Il Je, Hai Long Piao, Soon Ju Park, Sung Han Park, Chul Min Kim, Yuan Hu Xuan, Su Hyun Park, Jin Huang, Yang Do Choi, Gynheung An, Hann Ling Wong, Shozo Fujioka, Min-Chul Kim, Ko Shimamoto, Chang-deok Han
    • Journal Title

      Plant Cell 22

      Pages: 1777-1791

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The sterol methyltransferases SMT1, SMT2, and SMT3 influence Arabidopsis development through nonbrassinosteroid products.2010

    • Author(s)
      Francine Carland, Shozo Fujioka, Timothy Nelson
    • Journal Title

      Plant Physiol. 153

      Pages: 741-756

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana.2010

    • Author(s)
      Zhongxin Guo, Shozo Fujioka, Elison B.Blancaflor, Sen Miao, Xiaoping Gou, Jia Li
    • Journal Title

      Plant Cell 22

      Pages: 1161-1173

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ASKθ, a group-III Arabidopsis GSK3, functions in the brassinosteroid signalling pathway.2010

    • Author(s)
      Wilfried Rozhon, Juliane Mayerhofer, Elena Petutschnig, Shozo Fujioka, Claudia Jonak
    • Journal Title

      Plant J. 62

      Pages: 215-223

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana.2010

    • Author(s)
      Ho Yong Chung, Shozo Fujioka, Sunghwa Choe, Soyoun Lee, Youn Hyung Lee, Nam In Baek, In Sik Chung
    • Journal Title

      Plant Cell Rep. 29

      Pages: 397-402

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana2010

    • Author(s)
      Ho Yong Chung
    • Journal Title

      Plant Cell Reports

      Volume: 29 Pages: 397-402

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The sterol methyltransferases SMT1, SMT2, and SMT3 influence Arabidopsis development through nonbrassinosteroid products2010

    • Author(s)
      Francine Carland
    • Journal Title

      Plant Physiology

      Volume: 153 Pages: 741-756

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana2010

    • Author(s)
      Zhong Guo
    • Journal Title

      Plant Cell

      Volume: 22 Pages: 1161-1173

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RAV-Like1 maintains brassinosteroid homeostasis via the coordinated activation of BRI1 and biosynthetic genes in rice2010

    • Author(s)
      Byoung Il Je
    • Journal Title

      Plant Cell

      Volume: 22 Pages: 1777-1791

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intragenic suppression of a trafficking-defective brassinosteroid receptor mutant in Arabidopsis2010

    • Author(s)
      Youssef Belkhadir
    • Journal Title

      Genetics

      Volume: 185 Pages: 1283-1296

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of Arabidopsis brassinosteroid signaling by atypical basic helix-loop-helix proteins.2009

    • Author(s)
      Hao Wang, Yongyou Zhu, Shozo Fujioka, Tadao Asami, Jiayang Li, Jianming Li
    • Journal Title

      Plant Cell 21

      Pages: 3781-3791

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Antagonistic HLH/bHLH transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and Arabidopsis.2009

    • Author(s)
      Li-Ying Zhang, Ming-Yi Bai, Jinxia Wu, Jia-Ying Zhu, Hao Wang, Zhiguo Zhang, Wenfei Wang, Yu Sun, Jun Zhao, Xuehui Sun, Hongjuan Yang, Yunyuan Xu, Soo-Hwan Kim, Shozo Fujioka, Wen-Hui Lin, Kang Chong, Tiegang Lu, Zhi-Yong Wang
    • Journal Title

      Plant Cell 21

      Pages: 3767-3780

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The E3 ubiqutin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin effulux transporter PIN2 in Arabidopsis thaliana.2009

    • Author(s)
      Jianwei Pan, Shozo Fujioka, Jianling Peng, Jianghua Chen, Guangming Li, Rujin Chen
    • Journal Title

      Plant Cell 21

      Pages: 568-580

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Function of the α subunit of rice heterotrimeric G protein in brassinosteroid signaling.2009

    • Author(s)
      Katsuyuki Oki, Noriko Inaba, Kanako Kitagawa, Shozo Fujioka, Hidemi Kitano, Yukiko Fujisawa, Hisaharu Kato, Yukimoto Iwasaki
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 161-172

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The E3 ubiqutin ligase SCF^<TIR1/AFB> and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana2009

    • Author(s)
      Jianwei Pan
    • Journal Title

      Plant Cell 21

      Pages: 568-580

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antagonistic HLH/bHLH transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and Arabidopsis2009

    • Author(s)
      Li-Ying Zhang
    • Journal Title

      Plant Cell 21

      Pages: 3767-3780

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of Arabidopsis brassinosteroid signaling by atypical basic helix-loop-helix proteins2009

    • Author(s)
      Hao Wang
    • Journal Title

      Plant Cell 21

      Pages: 3781-3791

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of the α subunit of rice heterotrimetric G protein in brassinosteroid signaling2009

    • Author(s)
      Katsuyuki Oki
    • Journal Title

      Plant Cell Physiol 50

      Pages: 161-172

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brassinosteroids regulate grain filling in rice.2008

    • Author(s)
      Chuan-yin Wu, Anthony Trieu, Parthiban Radhakrishnan, Shing F. Kwok, Sam Harris, Ke Zhang, Jiulin Wang, Jianmin Wan, Huqu Zhai, Suguru Takatsuto, Shogo Matsumoto, Shozo Fujioka, Kenneth A.Feldmann, Roger I.Pennell
    • Journal Title

      Plant Cell 20

      Pages: 2130-2145

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Castasterone is a likely end product of brassinosteroid biosynthetic pathway in rice. Biochem. Biophys.2008

    • Author(s)
      Bo Kyung Kim, Shozo Fujioka, Suguru Takatsuto, Masafumi Tsujimoto, Sunghwa Choe
    • Journal Title

      Res.Commun. 374

      Pages: 614-619

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Castasterone is a likely end product of brassinosteroid biosynthetic pathway in rice.2008

    • Author(s)
      Bo Kyung Kim, Shozo Fujioka, Suguru Takatsuto, Masafumi Tsujimoto, Sunghwa Choe
    • Journal Title

      Biochem. Biophys. Res. Commun. 374

      Pages: 614-619

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Castasterone is a likely end product of brassinosteroid biosynthetic pathway in rice2008

    • Author(s)
      Bo Kyung Kim
    • Journal Title

      Biochem Biophys Res Commun 374

      Pages: 614-619

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brassinosteroids regulate grain filling in rice2008

    • Author(s)
      Chuan-yin Wu
    • Journal Title

      Plant Cell 20

      Pages: 2130-2145

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rice CYP734As function as multisubstrate and multifunctional enzymes in brassinosteroid catabolism.

    • Author(s)
      Tomoaki Sakamoto, Ayami Kawabe, Asako Tokida-Segawa, Bun-ichi Shimizu, Suguru Takatsuto, Yukihisa Shimada, Shozo Fujioka, Masaharu Mizutani
    • Journal Title

      Plant J. (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis.

    • Author(s)
      Yuhee Chung, Puna M.Maharjan, Oksun Lee, Shozo Fujioka, Suyoun Jang, Bokyung Kim, Suguru Takatsuto, Masafumi Tsujimoto, Hobang Kim, Seoae Cho, Taesung Park, Hyunwoo Cho, Ildoo Hwang, Sunghwa Choe
    • Journal Title

      Plant J. (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis

    • Author(s)
      Yuhee Chung
    • Journal Title

      Plant Journal

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CESTA, a positive regulator of brassinosteroid biosynthesis

    • Author(s)
      Brigitte Poppenberger
    • Journal Title

      EMBO Journal

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ASKθ, a group III Arabidopsis GSK3, functions in the brassinosteroid signalling pathway

    • Author(s)
      Wilfried Rozhon
    • Journal Title

      Plant Journal (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネのSHORT GRAIN 1遺伝子は器官の伸長を抑制するだけでなくブラシノステロイドに対する応答も抑制する2011

    • Author(s)
      中川仁
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台市
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネSHORT GRAIN 1遺伝子による器官サイズ制御へのBRシグナルの関与2010

    • Author(s)
      中川仁、田中惇訓、七夕高也、藤岡昭三、森昌樹
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-11-02
    • Related Report
      2010 Final Research Report
  • [Presentation] ブラシノステロイド情報伝達突然変異体bil4の原因遺伝子の細胞伸長における機能解析2010

    • Author(s)
      山上あゆみ、齊藤知恵子、中澤美紀、松井南、作田正明、藤岡昭三、中野明彦、浅見忠男、中野雄司
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-11-02
    • Related Report
      2010 Final Research Report
  • [Presentation] ブラシノステロイド情報伝達突然変異体bil4の原因遺伝子の細胞伸長における機能解析2010

    • Author(s)
      山上あゆみ
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-11-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネのSHORT GRAIN 1遺伝子による器官サイズ制御へのBRシグナルの関与2010

    • Author(s)
      中川仁
    • Organizer
      植物化学調節学会第45回大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-11-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] オーキシンはイネのブラシノステロイド感受性を高める2010

    • Author(s)
      坂本知昭、藤岡昭三
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2010 Final Research Report
  • [Presentation] イネとシロイヌナズナで異なる活性を示す合成ブラシノステロイドの解析2010

    • Author(s)
      中村郁子、藤岡昭三、辻本雅文、松岡信、吉田茂男、木下俊則、瀬戸秀春、中野雄司
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-18
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] CESTA, a positive regulator of brassinosteroid biosynthesis.2010

    • Author(s)
      Mamoona Khan, Wilfried Rozhon, Sigrid Husar, Gerhard Adam, Christian Luschnig, Shozo Fujioka, Tobias Sieberer, Brigitte Poppenberger
    • Organizer
      Plant Biology 2010
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] CESTA, a positive regulator of brassinosteroid biosynthesis2010

    • Author(s)
      Mamoona Khan
    • Organizer
      Plant Biology 2010
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネのSHORT GRAIN 1遺伝子は細胞分裂の抑制を介して器官のサイズを制御する2010

    • Author(s)
      中川仁
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] オーキシンはイネのブラシノライド感受性を高める2010

    • Author(s)
      坂本知昭
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規ブラシノステロイド不活性化経路の解明、2009

    • Author(s)
      水谷正治、川邊綾美、清水文一、嶋田幸久、藤岡昭三、坂本知昭
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Synthesis of hydroxylated sterols in transgenic Arabidopsis plants alters growth and steroid metabolism.2009

    • Author(s)
      Lisa Beste, Nurun Nahar, Kerstin Dalman, Shozo Fujioka, Lisbeth Jonsson, Paresh Dutta, Folke Sitbon
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Honolulu, Hawaii
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規ブラシノステロイド不活性化経路の解明2009

    • Author(s)
      水谷正治、川邉綾美、清水文一、嶋田幸久、藤岡昭三、坂本知昭
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Self-evaluation Report 2008 Annual Research Report
  • [Presentation] ブラシノステロイド情報伝達突然変異体bi14の原因遺伝子の機能解析2009

    • Author(s)
      山上あゆみ
    • Organizer
      植物化学調節学会大44回大会
    • Place of Presentation
      仙台
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規ブラシノステロイド不活性化経路の解明2009

    • Author(s)
      水谷正治
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規ブラシノステロイド情報伝達因子の単離2008

    • Author(s)
      坂本知昭、藤岡昭三、北野英己、松岡信
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] ブラシノステロイド応答におけるイネ3量体Gタンパク質αサブユニットの機能解析2008

    • Author(s)
      隠岐勝幸、稲葉規子、北川佳名子、藤岡昭三、北野英己、藤澤由紀子、加藤久晴、岩崎行玄
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional analysis of rice CYP85.2007

    • Author(s)
      Bo Kyung Kim, Shozo Fujioka, Suguru Takatsuto, Masafumi Tsujimoto, Sunghwa Choe
    • Organizer
      IPGSA 19th Meeting
    • Place of Presentation
      Puerto Vallarta, Mexico
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional analysis of rice CYP852007

    • Author(s)
      Bo Kyung Kim
    • Organizer
      IPGSA 19^<th> Meeting
    • Place of Presentation
      Mexico
    • Related Report
      2007 Annual Research Report
  • [Presentation] Synthesis of 9,11,11,12,12-deuterium Iabelled C21-and C19-steroids2007

    • Author(s)
      瀬戸 秀春
    • Organizer
      IPGSA 19^<th> Meeting
    • Place of Presentation
      Mexico
    • Related Report
      2007 Annual Research Report
  • [Presentation] ブラシノステロイドが茎の伸長と屈性を制御するメカニズム2007

    • Author(s)
      立川 巴
    • Organizer
      植物化学調節学会第42回大会
    • Place of Presentation
      静岡
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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