Functional genomics of protein phosphatases in yeast

• Project/Area Number: 19380193
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Osaka University
• Principal Investigator: HARASHIMA Satoshi Osaka University, 工学研究科, 教授 (70116086)
• Co-Investigator(Kenkyū-buntansha): KANEKO Yoshinobu 大阪大学, 工学研究科, 准教授 (90161182) ; SUGIYAMA Minetaka 大阪大学, 工学研究科, 助教 (80379130)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2007: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: プロテインホスファターゼ / 酵母 / リン酸化・脱リン酸化 / プロテインキナーゼ / 核局在 / プロテイホスファターゼ

Research Abstract

We have been engaged in a project to explore the function of all the protein phosphatases in S.cerevisiae. As a part of this project, we have investigated in this study the function of four protein phosphatases, Siw14, Yvh1 Ptp2 and Msg5. We have revealed that Siwl4 regulates localization and activity of transcriptional activator Gln3 and Yvh1 is involved in mRNA stability as well as rRNA processing. We have also clarified a mechanism by which Ptp2 and Msg5 are involved in calcium response in redundant manner.

Research Products

• [Journal Article] Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant. (2010)
  • Author(s): Hermansyah, Lavina W, Sugiyama M, Kaneko Y, Harashima S.
  • Journal Title: Arch Microbiol 192(3) Pages: 157-65
• [Journal Article] Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae. (2010)
  • Author(s): Hirasaki M, Nakamura F, Yamagishi K, Numamoto M, Shimada Y, Uehashi K, Muta S, Sugiyama M, Kaneko Y, Kuhara S, Harashima S.
  • Journal Title: J Biosci Bioeng 109(5) Pages: 433-41
  • NAID: 110007618917
• [Journal Article] Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant. (2010)
  • Author(s): Hermansyah, Lavina, WA., Sugiyama, M., Kaneko, Y., Harashima, S.
  • Journal Title: Arch Microbiol. 192 Pages: 157-165
  • Peer Reviewed
• [Journal Article] Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis. (2009)
  • Author(s): Hermansyah, Sugiyama M, Kaneko Y, Harashima S
  • Journal Title: Arch Microbiol 191(9) Pages: 721-33
• [Journal Article] Yeast protein phosphatases Ptp2p and Msg5p are involved in Gl-S transition, CLN2 transcription, and vacuole morphogenesis. (2009)
  • Author(s): Hermansyah, Sugiyama, M., Kaneko Y., Harashima, S.
  • Journal Title: Arch Microbiol. 191 Pages: 721-733
  • Peer Reviewed
• [Journal Article] Protein phosphatase Siw14 controlsintracellular localization of Gln3 in cooperation with Nprl kinase in Saccharomyces cerevisiae. (2008)
  • Author(s): Hirasaki M, Kaneko Y, Harashima S
  • Journal Title: Gene. 409(1-2) Pages: 34-43
• [Journal Article] Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Npr1 kinase in Saccharomyces cerevisiae. (2008)
  • Author(s): M. Hirasaki, Y. Kaneko, S. Harashima
  • Journal Title: Gene 409 Pages: 34-43
  • Peer Reviewed
• [Journal Article] Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Npr1 kinase in Saccharomyces cerevisiae. (2008)
  • Author(s): M. Hirasaki, Y. Kaneko, S. Harashima,
  • Journal Title: Gene 409 Pages: 34-43
  • Peer Reviewed
• [Presentation] 出芽酵母プロテインホスファターゼSiw14はGln3-Ure2複合体の解離を抑圧することでGln3の細胞内局在を制御する。 (2009)
  • Author(s): 沼本穂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-11
• [Presentation] Calcineurin and HOG pathway-independent suppression of the calcium sensitivity of the ptp2Δmsg5Δ protein phosphatase double disruptant by ssk2Δ disruption (2009)
  • Author(s): Lavina Walter Alvarez
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-11
• [Presentation] 出芽酵母プロテインホスファターゼSiw14によるGln3の細胞内局在制御 (2009)
  • Author(s): 沼本穂
  • Organizer: 第4回日本プロテインホスファターゼ研究会学術集会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2009-11-13
• [Presentation] The suppression of the calcium sensitivity of the ptp2Δmsg5Δ protein phosphatase double disruptant by ssk2Δ disruption is calcineurin and HOG pathway-independent (2009)
  • Author(s): Lavina Walter Alvarez
  • Organizer: 第4回日本プロテインボスファターゼ研究会学術集会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2009-11-13
• [Presentation] 出芽酵母プロテインホスファターゼSiw14による転写因子Gln3の機能制御 (2009)
  • Author(s): 沼本穂
  • Organizer: 第42回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2009-07-28
• [Presentation] Mechanism involved in the suppression of the calcium sensitivity of the protein phosphatase double disruptant, ptp2Δmsg5Δ by ssk2Δ disruption (2009)
  • Author(s): Lavina Walter Alvarez
  • Organizer: 第42回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2009-07-28
• [Presentation] Elucidation of the mechanism involved in the suppression of the calcium sensitivity of ptp2Δmsg5Δ protein phosphatase double disruptant by SSK2 (2009)
  • Author(s): Lavina Walter Alvarez
  • Organizer: 24^<th> International Conference on Yeast Genetics and Molecular Biology
  • Place of Presentation: イギリス マンチェスター
  • Year and Date: 2009-07-19
• [Presentation] Calcineurin & HOG pathway-independent suppression of the calcium sensitivity of the ptp2Δmsg5Δprotain phosphatase double disruptant by ssk2Δdisruption (2009)
  • Author(s): Walter Lavina, Hermansyah, 杉山峰崇, 金子嘉信原島俊
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 出芽酵母プロテインホスファターゼSiw14はGln3-Ure2複合体の解離を抑制することでGln3の細胞内局在を制御する (2009)
  • Author(s): 沼本穂, 杉山峰崇, 金子嘉信, 原島俊
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Satoshi Harashima Mechanism involved in the suppression of calcium sensitivity of PTP2ΔMSG5Δprotein phosphatase double disruptant by ssk2 disruption. (2009)
  • Author(s): Walter Lavina, Hermansyah, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima
  • Organizer: YEAST GENETICS AND MOLECULAR BIOLOGY NEWS JAPAN
  • Place of Presentation: つくば
• [Presentation] 出芽酵母△Siw14のカフェイン応答におけるGln3の転写活性とCell wall integrityの影響 (2008)
  • Author(s): 沼本穂, 平崎正孝, 杉山峰崇, 金子嘉信, 原島俊
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Functional Genomics of Protein Phosphatases in a Single Celled Eukaryote. (2008)
  • Author(s): Masataka Hirasaki, Hermansyah, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima
  • Organizer: 8th International Conference on Protein Phosphatases
  • Place of Presentation: 前橋
• [Presentation] 出芽酵母脱リン酸化酵素Siw14のカフェイン、ラバマイシン応答における機能 (2008)
  • Author(s): 沼本穂, 平崎正孝, 杉山峰崇, 金子嘉信, 原島俊
  • Organizer: 日本生物工学会第60回大会
  • Place of Presentation: 仙台
• [Presentation] 出芽酵母プロテインホスファターゼSiw14による転写因子Gln3の機能制御 (2008)
  • Author(s): 沼本穂, 杉山峰崇, 金子嘉信, 原島俊
  • Organizer: 酵母遺伝学フォーラム第42回研究報告会
  • Place of Presentation: つくば
• [Presentation] Double deletion of PTP2 and MSG5 genes encoding protein phophatases causes a defect in vacuolar morphology in Saccharomyces cerevisiae. (2008)
  • Author(s): Hermansyah, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima
  • Organizer: 2008 YEAST GENETICS AND MOLECULAR BIOLOGY MEETING
  • Place of Presentation: Toronto
• [Presentation] Protein kinases whose disruption suppress calcium sensitivity of protein phosphatase deltaptp2 deltamsg5 double disruptain in Saccharomyces cerevisiae (2007)
  • Author(s): Hermansyah, 杉山峰崇, 金子嘉信, 原島俊
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] 酵母プロテインホスファターゼSiw14はプロテインキナーゼNpr1と共にGln3の細胞内局在を制御する (2007)
  • Author(s): 平崎正孝, 金子嘉信, 原島俊
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] 出芽酵母プロテインホスファターゼYvhlpの欠失は35S rRNA前駆体の蓄積を引き起こす (2007)
  • Author(s): 杉山峰崇, 酒井大樹, 原島俊
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] 出芽酵母プロテインホスファターゼYvhlpの欠失は35S rRNA前駆体の蓄積を引き起こす (2007)
  • Author(s): 杉山峰崇, 酒井大樹, 原島俊
  • Organizer: 酵母遺伝学フォーラム第40回研究報告会
  • Place of Presentation: 大阪
• [Presentation] Systematic screening of protein kinase gene disruption as suppressor for calcium sensitivity of ptp2 msg5 protein phosphatase double mutation in Saccharomyces cerevisiae0 (2007)
  • Author(s): Hermansyah, 杉山峰崇, 金子嘉信, 原島俊
  • Organizer: 酵母遺伝学フォーラム第40回研究報告会
  • Place of Presentation: 大阪
• [Presentation] プロテインホスファターゼSiw14はプロテインキナーゼNpr1と共にGln3の細胞内局在を制御する (2007)
  • Author(s): 平崎正孝, 金子嘉信, 原島俊
  • Organizer: 酵母遺伝学フォーラム第40回研究報告会
  • Place of Presentation: 大阪
• [Presentation] Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Nprl kinase in Saccaromyces cerevisiae. (2007)
  • Author(s): asataka Hirasaki, Yoshinobu Kaneko, Satoshi Harashima
  • Organizer: EuroPhosphatases 2007
  • Place of Presentation: Aveiro (Portugal)